ISMbo1

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform ISMt3Synonym(s)
Accession numberTranspositionOriginHost
AP010918 ND Mycobacterium bovis
Mycobacterium bovis BCG Tokyo 172
Mycobacterium bovis BCG
DNA section
IS Length : 2212 bp

Ends


IR Length : 38/50

IRL : TGTCGACGGCACGTGAAAACTGACCCCGGCGCGGCACCCGAATTTTGACC
IRR : TGTCAACGGCACCCGAAAACTGACCCCCTGACGGCATCTGAAAATTGACC

Insertion site


Left flankDirect repeatRight flankDR Length
CTGCTGCTTACCGTTCACAAGGGCG5

DNA sequence

TGTCGACGGCACGTGAAAACTGACCCCGGCGCGGCACCCGAATTTTGACCCCCTGGTCGGGTGGACTGGCTCTACCCGAGCCAGGAGGACCGAAGGGAAT
GTTGACTGTGGAAGATTGGGCTGAGATTCGCCGATTGCATCGCGCGGAGGGTTTGCCGATCAAGATGATCGCCCGGGTGCTGGGGATTTCCAAGAACACG
GTGAAGTCAGCGTTGGAATCAAACCAGCAGCCGAAATATGAACGGGCACCGCAGGGTTCGATCGTTGATGCGGTTGAGCCGCGGATCCGGGAGTTGTTGC
AGGCCTATCCGACGATGCCGGCGACGGTGATCGCCGAGCGGATCGGCTGGGAGCGCTCGATTCGGGTGCTCTCGGCGCGGGTGGCCGAGCTGCGCCCGGT
GTATCTGCCGCCGGACCCGGCGTCGCGCACCACGTATGTGGCAGGCGAAATTGCCCAGTGCGACTTCTGGTTTCCGCCGATCGAGTTGCCGGTAGGGTTC
GGGCAGACCCGCACGGCCAAACAGTTGCCGGTGCTGACCATGGTGTGCGCCTATTCGCGCTGGCTGTTGGCGATGCTGCTGCCCAGCAGGTGTGCCGAGG
ACCTGTTCGCCGGCTGGTGGCGGCTGATCGAGGCGTTGGGGGCGGTGCCGCGGGTGTTGGTGTGGGATGGCGAGGGCGCGATCGGGCGCTGGCGCGGCGG
GCGGTCGGAGTTGACCACTGAGTGTCAGGCGTTCCGCGGCACGCTGGCGGCCAAGGTGCTCATCTGCCGGCCGGCCGACCCGGAGGCCAAGGGCCTCATT
GAACGGGCCCACGACTACCTGGAGCGCTCGTTTTTGCCCGGGCGGGTGTTTGCCTCGCCGGCCGATTTCAACGCCCAACTGGGCGCCTGGCTGGCGCTGG
TGAACACCCGCACCCGCCGGGCGCTGGGTTGTGCGCCCACCGATCGCATCGGCGCGGATCGGGCCGCGATGCTGAGCTTGCCGCCGGTGGCGCCGGCCAC
CGGGTGGTGCACCTCGCTGCGGCTGCCCCGGGATCACTATGTGCGCTGCGATTCCAACGACTACTCGGTGCACCCGGGTGTGATCGGGCATCGGGTGCTG
GTGCGCGCCGACCTGGAGCGGGTGCATGTGTTCTGCGACGGTGAGCTGGTCGCCGACCACGAGCGGATCTGGGCGGTCCATCAGACGGTCTCCGATCCCG
CACATGTGGAGGCGGCGAAGGTGTTGCGCCGCCGGCACTTCAGTGCAGCATCACCGGTAGTTGAGCCGCAGGTGCAGGTCCGCTCACTGAGCGACTACGA
TGACGCGCTGGGAGTCGACATCGATGGCGGGGTGGCCTGATGCCCACCACCAAAGCCACCCAGCGCCGTGATGTTTCCACCGAGATCGCTTACCTGACAA
GAGCATTGAAAGCTCCCACCCTGCGTGAGTCAGTGTCCCGGCTGGCCGATCGCGCCCGCGCCGAGAACTGGAGCCACGAAGAATACCTGGCCGCCTGCCT
GCAGCGGGAAGTGTCAGCCCGGGAGTCCCATGGTGGTGAGGGCCGCATCCGCGCCGCCCGCTTCCCGGCTCGGAAGTCGTTGGAAGAGTTCGACTTTGAG
CATGCTCGTGGCCTCAAACGCGACACCATCGCACATCTGGGCACCCTGGATTTCATCACCGCCCGCGATAACGTCGTGTTTTTGGGCCCCGCTGGCACCG
GGAAGACTCATCTTGCGGTCGGCCTGGCGATACGCGCGTGTCAGGCCGGTCATCGGGTGCTGTTCGCCACCGCCGCCGAATGGGTAGCACGGCTCGCCGA
GGCTCACCACGCCGGGCGCATCTACGCCGAACTCACCCGGCTTTGCCGCTATCCGCTCCTGGTGGTTGACGAAGTCGGCTACATTCCGTTTGAGCCCGAG
GCCGCCAACCTCTTCTTCCAGCTGGTGTCCTCCCGGTATGAGCGGGCCAGCTTGATCGTCACGTCCAATAAGGCCTTCGGCCGGTGGGGCGAGGTTTTCG
GCGGCGACGACGTCGTTGCTGCCGCCATGATCGACCGCCTCGTCCACCATGCTGAAGTCGTCGCCCTCAAAGGCGACAGCTACCGGCTCAAAGACCGCGA
CCTCGGCCGCGTCCCACCAGCCGGAACCACCGAAGAATAACCACCAACCGCCCGGTCTAGGGGGTCAATTTTCAGATGCCGTCAGGGGGTCAGTTTTCGG
GTGCCGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1242 bp413 aa991340+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MLTVEDWAEIRRLHRAEGLPIKMIARVLGISKNTVKSALESNQQPKYERAPQGSIVDAVEPRIRELLQAYPTMPATVIAERIGWERSIRVLSARVAELRP
VYLPPDPASRTTYVAGEIAQCDFWFPPIELPVGFGQTRTAKQLPVLTMVCAYSRWLLAMLLPSRCAEDLFAGWWRLIEALGAVPRVLVWDGEGAIGRWRG
GRSELTTECQAFRGTLAAKVLICRPADPEAKGLIERAHDYLERSFLPGRVFASPADFNAQLGAWLALVNTRTRRALGCAPTDRIGADRAAMLSLPPVAPA
TGWCTSLRLPRDHYVRCDSNDYSVHPGVIGHRVLVRADLERVHVFCDGELVADHERIWAVHQTVSDPAHVEAAKVLRRRHFSAASPVVEPQVQVRSLSDY
DDALGVDIDGGVA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
801 bp266 aa13402140+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MPTTKATQRRDVSTEIAYLTRALKAPTLRESVSRLADRARAENWSHEEYLAACLQREVSARESHGGEGRIRAARFPARKSLEEFDFEHARGLKRDTIAHL
GTLDFITARDNVVFLGPAGTGKTHLAVGLAIRACQAGHRVLFATAAEWVARLAEAHHAGRIYAELTRLCRYPLLVVDEVGYIPFEPEAANLFFQLVSSRY
ERASLIVTSNKAFGRWGEVFGGDDVVAAAMIDRLVHHAEVVALKGDSYRLKDRDLGRVPPAGTTEE

 

Blast result :
Comments
The previous sequence (accession number : AJ238712) was truncated at the 5' end and was replaced by a complete copy found in a sequenced genome (July 2012).
References
1] Lefevre, P., Braibant, M., Content, J. and gilot, P. (1999) FEMS Microbiol.Lett. 178, 211-217
2] Seki,M., Honda,I., Fujita,I., Yano,I., Yamamoto,S. and Koyama,A. (2009) Vaccine 27 (11), 1710-1716