ISMav1
- Family IS21
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
AF125999 | ND | Mycobacterium avium | Mycobacterium avium subsp. silvaticum |
DNA section
IS Length : 2536 bp
Ends
IR Length : 41/52
IRL : TGTCCATGGCCATGAGAAAGTCCCCATCTGTGGCCAGTAGGAAGTCCCCA
IRR : TGTCAATGGCCAAGTAGAAGTCCCCGCTGGTGGCCAGCAGAAGTCCCCAC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CGCGCGCGAA | TGCAC | TTAATGCGTG | 5 |
DNA sequence
TGTCCATGGCCATGAGAAAGTCCCCATCTGTGGCCAGTAGGAAGTCCCCACTGGTGGCCAGGTAGAAGTCCCCACTCCTTTGCGGGCTCGTCGTGTCTTT
TCCGGGAGCTTGAGGGCCTGGGCGGTGACGGTGATAGCGCCAACTGTCACTTGATCACCGCCCAGGGAGCTCTATTGAAGTCTGCAAGGGAGCGTATGAA
CATCATTTCGGCGTATCACCAGGTCGGGTCGTATCGCGGCGCGGCCGAGCTGTGCGGTACTACGCACAAGACGGTCAAGCGGGTCATCGAGCGGGCCGAG
GCCGGTGGCGCGCCCCCGCGGGAACCACGGCCACGCAACCTCGACGCGTTCACCGATCTAGTCGCCACCCGAGTCGAGAAATCACACGGCAAGATGTCGG
CGAAGCGGATGCTGCCGATCGCCCGAGCTGCCGGGTATCAGGGCTCGGCCCGTAACTTCCGCCGCCTGGTAGCCGAGCAGAAAGTATTGTGGCGCAACGC
TAACCGGCATCAACGCCGTCCGGCGGTCTGGTCACCCGGTGACTATCTGGTGATGGATTGGGCCGAAGCTGCACCGGGGTTGATGGTGTTCTGCGCGGTG
CTGGCCTATTCGCGGTGGCGGTTTGTGCGGTTCGCCGCCGACGAGAAAGCCTCGACCACGCTGGCGATGATCGCCGAAGCCCTCGAGGCGATCGGTGGGG
TTCCGGCCAAGATCCTGGCCGACCGGATGGGCTGCCTCAAAGGTGGTGTCGTCGCCAATGTTGTTGTTCCAACACCGGATTATGTGCGATTCGCGTCCCA
CTATGGCTTCGTTCCGGACTTCTGCCACGGTGCGGATCCGCAATCGAAGGGCATCGTGGAGAACCTCTGTGGCTACGCTCAGGACGACCTTGCGGTGCCG
CTGCTGACCGAAGCTGCGTTAGCCGGTGAGCAGGTCGACCTACGTGCCCTCAACGCCCAGGCGCAACTATGGTGCGCCGAGGTCAATGCCACGGTCCACT
CGGAGATCTGCGCCGTGCCCAACGATCGCTTGGTTGACGAGCGCACCGTCTTGAGGGAGCTGCCCTCGCTGCGGCCGACGATCGGCTCGGGGTCGGTGCG
CCGTAAGGTCGACGGCCTCTCGTGCATCCGTTACGGCTCAGCTCGTTACTCGGTGCCTCAGCGGCTCGTCGGTGCCACCGTGGCGGTGGTGGTCGATCAT
GGCGCCCTGATCCTGTTGGAACCTGCGACCGGTGTGATCGTGGCCGAGCACGAGCTCGTCAGCCCAGGTGAGGTGTCCATCCTCGATGAACACTACGACG
GACCCAGACCCGCACCCTCGCGTGGTCCTCGCCCGAAAAACCCAAGCAGAGAAACGATTCTGCGCATTGGGAACCGAAGCGCAAGCAAGTTCCTCGTCGG
TGCTGCTGCGATCGGCAACACCCGACTGAAATCCGAACTCGACATTCTGCTCGGCCTTGGCGCCGCCCACGGCGAACAGGCTTTGATTGACGCGCTGCGC
CGGACGGTTGCGTTTCGCCGGTTCCGCGCTGCCGACGTGCGCTCGATCCTGGCCGCCGGCGCCGGCACCCCACAACCCCGCCCCGCCGGCGACGCACTCG
TGCTCGATCTGCCCACCGTCGAGACCCGCTCGTTGGAGGCCTACAAGATCAACACCACCGACGGGACGGCCTCATGACCACCGCTGCCAAGCCGGTGGCA
CCGTCCTCGGCGGCACCGCTGGCTGCTGACCTTGACGCGGCGCTGCGGCGGTTGAAGCTGGCCACGGTGCGCCGCAACGCCGCCGAGGTGTTGCAAGTCG
CCAAGACGCAACGCTGGACACCGGAGGAGATCCTGCGGACGTTGGTTGAGGCCGAGATCGCTGCCCGCGATGCCTCCAACACCGCCAACCGTCTCAAGGC
CGCAGCCTTCCCGGTCACCAAGACCCTCGACGGGTTCGACGTCACCGGATCGTCGATCACCGCAGCCACGTTCGACTACCTGTCGAGCCTGGAATGGATT
CGGGCACAACAGAACCTGGCGGTCATTGGCCCACCTGGTACGGGCAAAAGTCACCTGCTCATCGGCTGCGGGCACGCTGCCGTCCACGCCGGATTCAAAG
TCCGCTACTTCACCGCCGCCGACCTGATCGAGGTCCTCTACCGCGGCCTGGCCGACAACACCGTCGGCAAGATCATCGACACCCTGCTCCGCGCGGATCT
GGTCATCTTGGACGAGATCGGCTTCGCCCCGCTCGACGACACCGGGACTCAACTGTTGTTCCGGCTCGTGGCTGCCGGCTACGAGCGCCGCTCCCTGGCC
ATCGCCTCGCATTGGCCCTTCGAACAATGGGGGCGATTCCTGCCCGAGCACACCACCGCCGCCAGCATCCTCGATCGGCTGCTGCACCACGCCAGCATCG
TCGTCACCTCCGGCGAGTCCTACCGGATGCGCCACGCCGACCACAAGAAGGGAGCCGCCAAGAATTAGCCAACCACCCGCAGCGGAGTGGGGACTTCTGC
TGGCCACCAGCGGGGACTTCTACTTGGCCATTGACA
TCCGGGAGCTTGAGGGCCTGGGCGGTGACGGTGATAGCGCCAACTGTCACTTGATCACCGCCCAGGGAGCTCTATTGAAGTCTGCAAGGGAGCGTATGAA
CATCATTTCGGCGTATCACCAGGTCGGGTCGTATCGCGGCGCGGCCGAGCTGTGCGGTACTACGCACAAGACGGTCAAGCGGGTCATCGAGCGGGCCGAG
GCCGGTGGCGCGCCCCCGCGGGAACCACGGCCACGCAACCTCGACGCGTTCACCGATCTAGTCGCCACCCGAGTCGAGAAATCACACGGCAAGATGTCGG
CGAAGCGGATGCTGCCGATCGCCCGAGCTGCCGGGTATCAGGGCTCGGCCCGTAACTTCCGCCGCCTGGTAGCCGAGCAGAAAGTATTGTGGCGCAACGC
TAACCGGCATCAACGCCGTCCGGCGGTCTGGTCACCCGGTGACTATCTGGTGATGGATTGGGCCGAAGCTGCACCGGGGTTGATGGTGTTCTGCGCGGTG
CTGGCCTATTCGCGGTGGCGGTTTGTGCGGTTCGCCGCCGACGAGAAAGCCTCGACCACGCTGGCGATGATCGCCGAAGCCCTCGAGGCGATCGGTGGGG
TTCCGGCCAAGATCCTGGCCGACCGGATGGGCTGCCTCAAAGGTGGTGTCGTCGCCAATGTTGTTGTTCCAACACCGGATTATGTGCGATTCGCGTCCCA
CTATGGCTTCGTTCCGGACTTCTGCCACGGTGCGGATCCGCAATCGAAGGGCATCGTGGAGAACCTCTGTGGCTACGCTCAGGACGACCTTGCGGTGCCG
CTGCTGACCGAAGCTGCGTTAGCCGGTGAGCAGGTCGACCTACGTGCCCTCAACGCCCAGGCGCAACTATGGTGCGCCGAGGTCAATGCCACGGTCCACT
CGGAGATCTGCGCCGTGCCCAACGATCGCTTGGTTGACGAGCGCACCGTCTTGAGGGAGCTGCCCTCGCTGCGGCCGACGATCGGCTCGGGGTCGGTGCG
CCGTAAGGTCGACGGCCTCTCGTGCATCCGTTACGGCTCAGCTCGTTACTCGGTGCCTCAGCGGCTCGTCGGTGCCACCGTGGCGGTGGTGGTCGATCAT
GGCGCCCTGATCCTGTTGGAACCTGCGACCGGTGTGATCGTGGCCGAGCACGAGCTCGTCAGCCCAGGTGAGGTGTCCATCCTCGATGAACACTACGACG
GACCCAGACCCGCACCCTCGCGTGGTCCTCGCCCGAAAAACCCAAGCAGAGAAACGATTCTGCGCATTGGGAACCGAAGCGCAAGCAAGTTCCTCGTCGG
TGCTGCTGCGATCGGCAACACCCGACTGAAATCCGAACTCGACATTCTGCTCGGCCTTGGCGCCGCCCACGGCGAACAGGCTTTGATTGACGCGCTGCGC
CGGACGGTTGCGTTTCGCCGGTTCCGCGCTGCCGACGTGCGCTCGATCCTGGCCGCCGGCGCCGGCACCCCACAACCCCGCCCCGCCGGCGACGCACTCG
TGCTCGATCTGCCCACCGTCGAGACCCGCTCGTTGGAGGCCTACAAGATCAACACCACCGACGGGACGGCCTCATGACCACCGCTGCCAAGCCGGTGGCA
CCGTCCTCGGCGGCACCGCTGGCTGCTGACCTTGACGCGGCGCTGCGGCGGTTGAAGCTGGCCACGGTGCGCCGCAACGCCGCCGAGGTGTTGCAAGTCG
CCAAGACGCAACGCTGGACACCGGAGGAGATCCTGCGGACGTTGGTTGAGGCCGAGATCGCTGCCCGCGATGCCTCCAACACCGCCAACCGTCTCAAGGC
CGCAGCCTTCCCGGTCACCAAGACCCTCGACGGGTTCGACGTCACCGGATCGTCGATCACCGCAGCCACGTTCGACTACCTGTCGAGCCTGGAATGGATT
CGGGCACAACAGAACCTGGCGGTCATTGGCCCACCTGGTACGGGCAAAAGTCACCTGCTCATCGGCTGCGGGCACGCTGCCGTCCACGCCGGATTCAAAG
TCCGCTACTTCACCGCCGCCGACCTGATCGAGGTCCTCTACCGCGGCCTGGCCGACAACACCGTCGGCAAGATCATCGACACCCTGCTCCGCGCGGATCT
GGTCATCTTGGACGAGATCGGCTTCGCCCCGCTCGACGACACCGGGACTCAACTGTTGTTCCGGCTCGTGGCTGCCGGCTACGAGCGCCGCTCCCTGGCC
ATCGCCTCGCATTGGCCCTTCGAACAATGGGGGCGATTCCTGCCCGAGCACACCACCGCCGCCAGCATCCTCGATCGGCTGCTGCACCACGCCAGCATCG
TCGTCACCTCCGGCGAGTCCTACCGGATGCGCCACGCCGACCACAAGAAGGGAGCCGCCAAGAATTAGCCAACCACCCGCAGCGGAGTGGGGACTTCTGC
TGGCCACCAGCGGGGACTTCTACTTGGCCATTGACA
Protein section
ORF number : 2
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1504 bp | 500 aa | 174 | 1677 | + | No |
Chemistry : DDE
ORF sequence :
MKSARERMNIISAYHQVGSYRGAAELCGTTHKTVKRVIERAEAGGAPPREPRPRNLDAFTDLVATRVEKSHGKMSAKRMLPIARAAGYQGSARNFRRLVA
EQKVLWRNANRHQRRPAVWSPGDYLVMDWAEAAPGLMVFCAVLAYSRWRFVRFAADEKASTTLAMIAEALEAIGGVPAKILADRMGCLKGGVVANVVVPT
PDYVRFASHYGFVPDFCHGADPQSKGIVENLCGYAQDDLAVPLLTEAALAGEQVDLRALNAQAQLWCAEVNATVHSEICAVPNDRLVDERTVLRELPSLR
PTIGSGSVRRKVDGLSCIRYGSARYSVPQRLVGATVAVVVDHGALILLEPATGVIVAEHELVSPGEVSILDEHYDGPRPAPSRGPRPKNPSRETILRIGN
RSASKFLVGAAAIGNTRLKSELDILLGLGAAHGEQALIDALRRTVAFRRFRAADVRSILAAGAGTPQPRPAGDALVLDLPTVETRSLEAYKINTTDGTAS
EQKVLWRNANRHQRRPAVWSPGDYLVMDWAEAAPGLMVFCAVLAYSRWRFVRFAADEKASTTLAMIAEALEAIGGVPAKILADRMGCLKGGVVANVVVPT
PDYVRFASHYGFVPDFCHGADPQSKGIVENLCGYAQDDLAVPLLTEAALAGEQVDLRALNAQAQLWCAEVNATVHSEICAVPNDRLVDERTVLRELPSLR
PTIGSGSVRRKVDGLSCIRYGSARYSVPQRLVGATVAVVVDHGALILLEPATGVIVAEHELVSPGEVSILDEHYDGPRPAPSRGPRPKNPSRETILRIGN
RSASKFLVGAAAIGNTRLKSELDILLGLGAAHGEQALIDALRRTVAFRRFRAADVRSILAAGAGTPQPRPAGDALVLDLPTVETRSLEAYKINTTDGTAS
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
795 bp | 264 aa | 1674 | 2468 | + | No |
AG : IS21 helper
ORF sequence :
MTTAAKPVAPSSAAPLAADLDAALRRLKLATVRRNAAEVLQVAKTQRWTPEEILRTLVEADAEIAARDASNTANRLKAAAFPVTKTLDGFDVTGSSITAA
TFDYLSSLEWIRAQQNLAVIGPDAPGTGKSHLLIGCGHAAVHAGFKVRYFTAADLIEVLYRGLADNTVGKIIDTLLRADLVILDDAEIGFAPLDDTGTQL
LFRLVAAGYERRSLAIASHWPFEQWGRFLPEHTTAASILDRLLHHADASIVVTSGESYRMRHADHKKGAAKN
TFDYLSSLEWIRAQQNLAVIGPDAPGTGKSHLLIGCGHAAVHAGFKVRYFTAADLIEVLYRGLADNTVGKIIDTLLRADLVILDDAEIGFAPLDDTGTQL
LFRLVAAGYERRSLAIASHWPFEQWGRFLPEHTTAASILDRLLHHADASIVVTSGESYRMRHADHKKGAAKN
Blast result :
Comments
Internal DR:
L1: TGGCCATGAGAAAGTCCCCATCTG (7-30)
L2: TGGCCAGTAGGAAGTCCCCACTGG (31-54)
L3: TGGCCAGGTAGAAGTCCCCACTCC (55-78)
R1: TGGCCAAGTAGAAGTCCCCGCTGG CS (2530-2507)
R2: TGGCCAGCAGAA-GTCCCCACTCC CS (2506-2484)
The elimination of a potential sequencing mistake (T175) in the ORF1 allows a N-terminal extension (MKSARER) highly homologous to the one of IS1415 (MKSSREI) with a good RBS (AGGGAG: 164-169).
L1: TGGCCATGAGAAAGTCCCCATCTG (7-30)
L2: TGGCCAGTAGGAAGTCCCCACTGG (31-54)
L3: TGGCCAGGTAGAAGTCCCCACTCC (55-78)
R1: TGGCCAAGTAGAAGTCCCCGCTGG CS (2530-2507)
R2: TGGCCAGCAGAA-GTCCCCACTCC CS (2506-2484)
The elimination of a potential sequencing mistake (T175) in the ORF1 allows a N-terminal extension (MKSARER) highly homologous to the one of IS1415 (MKSSREI) with a good RBS (AGGGAG: 164-169).
References
1] Tizard, M., Bull, T., Millar, D., Doran, T., Martin, H., Sumar, N., Ford, J. and Hermon-Taylor, J. (1998) Microbiology. 144(Pt 12): p. 3413-23.
1] Berger, B. (2000) Ph.D. Thesis. University of Lausanne, Switzerland.
1] Berger, B. (2000) Ph.D. Thesis. University of Lausanne, Switzerland.