ISMcte1
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
ND | Micrococcus terreus | Micrococcus terreus 1AStreptC4R3 Microbacterium sediminis YLB-01 plasmid unnamed Microbacterium luteum A18JL200 |
DNA section
IS Length : 1307 bp
Ends
IR Length : 16
IRL : GGCGTGTTGATCAAGAAGGTGCGGGGTGGCCAGCCGCCGTGAGTTGCGCG
IRR : GGCGTGTTGATCAAGAGGTTCCGCTCCTCGGTGGGTGAGTCTTGAACGGT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GCCCCTCA | CTAG | GGCTGCAG | 4 |
AACACCCCACCCCATATCAG | CTGATATGGGGTGGGGTCTT | 0 | |
GCGCTGCTAGACAGGA | CTAG | GTCTGTCCTCCATGCT | 4 |
DNA sequence
GGCGTGTTGATCAAGAAGGTGCGGGGTGGCCAGCCGCCGTGAGTTGCGCGGGAGAATGGGCGTGTGAAGATCCAGGTGCTGCATATCGAGGATTGCCCGA
ATTGGCAGGAGGCGGGCGATCGAGTACGGCTGGTGCTCGACTCGATCGGACGGGGCGATGTTCCGGTGGAGTTCGTGTTGATCCGCACCGAGGCTGAGGC
GGTGAGCTCTGGTTTCGCGGGCTCGCCCACGATTCTTCTGGACGGTCAGGACTTGTTCCCGTCCCAGGGGAACACTGCCGACTTGGCGTGCAGGGTCTAT
CTGACCGAGCGCGGCTTTGCGGGTGCGCCGACGGTGGGACAGCTTGAGCGCACGCTGCAGGAGCGCGAAGCGCTCAGCGGAGATCGGTCTTGATCCAGAT
CAGCGCCGCGGCGAGGCTGACGGCGGCTCGGTAGTTGCGGGCGAGTTTGTCCGAGCGCATCGCGATGCCGCGCCACTGCTTGAGACGGTTGAAGCAGCGT
TCTACGACGTTGCGTCCCTTGTAGCGTTCCTTCTGCTTGTCGCCGAAAGCGATCGGCCGGCCCGGCTTCTTGCGGCGGTGCGCGATCTGGTCGTCCCGCT
CGGGGATGGTCGCCGCGATCCTCCGGTCGCGGAGCCAGGCGCGGTTCGCCCTTGAGGGGTACCCCTTGTCGGCGAGCACCCGGTCGGGGTGGGTGCGGGG
GCGGCCCTTCCCGTCGCGGGGAACGCGGATGTTCTCCAGCACCGCGCTCATCATCGTGGTGTCGTTGATGTTCCCGGCGGTGATCATCATGCTCAGCGGC
CGGCCGCGGCCGTCGCAGACCAGGTGCAGTTTGGTCGTCAGCCCGCCGCGGGAGCGTCCGATCCCGTGATCAGGCGGCTCGGACCACGGATTCTTGTGAT
TCGGCACAGCCCCCTGTGTCCCGGGCGAGGGTCGCGCCGTGCTGATGCACGCGCGCGATCGTGGAGTCGATCGAGACGACCCAGTCGATTTCCCCAGCGG
CGTCGGCCTGCTTCTGCACCTCGGTGAGCAGCTTCTGCCAGGTGCCGTCCCCGGCCCACCGGTTGAACCGCTTGTAGATCGAGTTCCACTTCCCGAACCG
TTCCGGAACGTCACGCCACGGCGCACCGGTCCGGTACTTCCACGCCATCCCCTCGATAGCGAGCCGGTGATCCGTCCACGGCCGCGACCGACCGGTCACC
GTCGGCATCAACGGCTCCATCACGGCCCACACCTCGTCAGAGATCTCCTGTCGCGTCACCGTTCAAGACTCACCCACCGAGGAGCGGAACCTCTTGATCA
ACACGCC
ATTGGCAGGAGGCGGGCGATCGAGTACGGCTGGTGCTCGACTCGATCGGACGGGGCGATGTTCCGGTGGAGTTCGTGTTGATCCGCACCGAGGCTGAGGC
GGTGAGCTCTGGTTTCGCGGGCTCGCCCACGATTCTTCTGGACGGTCAGGACTTGTTCCCGTCCCAGGGGAACACTGCCGACTTGGCGTGCAGGGTCTAT
CTGACCGAGCGCGGCTTTGCGGGTGCGCCGACGGTGGGACAGCTTGAGCGCACGCTGCAGGAGCGCGAAGCGCTCAGCGGAGATCGGTCTTGATCCAGAT
CAGCGCCGCGGCGAGGCTGACGGCGGCTCGGTAGTTGCGGGCGAGTTTGTCCGAGCGCATCGCGATGCCGCGCCACTGCTTGAGACGGTTGAAGCAGCGT
TCTACGACGTTGCGTCCCTTGTAGCGTTCCTTCTGCTTGTCGCCGAAAGCGATCGGCCGGCCCGGCTTCTTGCGGCGGTGCGCGATCTGGTCGTCCCGCT
CGGGGATGGTCGCCGCGATCCTCCGGTCGCGGAGCCAGGCGCGGTTCGCCCTTGAGGGGTACCCCTTGTCGGCGAGCACCCGGTCGGGGTGGGTGCGGGG
GCGGCCCTTCCCGTCGCGGGGAACGCGGATGTTCTCCAGCACCGCGCTCATCATCGTGGTGTCGTTGATGTTCCCGGCGGTGATCATCATGCTCAGCGGC
CGGCCGCGGCCGTCGCAGACCAGGTGCAGTTTGGTCGTCAGCCCGCCGCGGGAGCGTCCGATCCCGTGATCAGGCGGCTCGGACCACGGATTCTTGTGAT
TCGGCACAGCCCCCTGTGTCCCGGGCGAGGGTCGCGCCGTGCTGATGCACGCGCGCGATCGTGGAGTCGATCGAGACGACCCAGTCGATTTCCCCAGCGG
CGTCGGCCTGCTTCTGCACCTCGGTGAGCAGCTTCTGCCAGGTGCCGTCCCCGGCCCACCGGTTGAACCGCTTGTAGATCGAGTTCCACTTCCCGAACCG
TTCCGGAACGTCACGCCACGGCGCACCGGTCCGGTACTTCCACGCCATCCCCTCGATAGCGAGCCGGTGATCCGTCCACGGCCGCGACCGACCGGTCACC
GTCGGCATCAACGGCTCCATCACGGCCCACACCTCGTCAGAGATCTCCTGTCGCGTCACCGTTCAAGACTCACCCACCGAGGAGCGGAACCTCTTGATCA
ACACGCC
Recoding section
- Recoding by frameshift
- Frame -1
- Type translational
- Experimentally demonstrated No
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
390 bp | 129 aa | 49 | 438 | + | No |
Description : First part of the transposase
ORF sequence :
MTRQEISDEVWAVMEPLMPTVTGRSRPWTDHRLAIEGMAWKYRTGAPWRDVPERFGKWNSIYKRFNRWAGDGTWQKLLTEVQKQADAAGEIDWVVSIDST
IARVHQHGATLARDTGGCAESQESVVRAA
IARVHQHGATLARDTGGCAESQESVVRAA
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
822 bp | 273 aa | 113 | 934 | + | No |
Description : Second part of the transposase
ORF sequence :
PVGRGRGRITGSLSRGWRGSTGPVRRGVTFRNGSGSGTRSTSGSTGGPGTAPGRSCSPRCRSRPTPLGKSTGSSRSTPRSRACISTARPSPGTQGAVPNH
KNPWSEPPDHGIGRSRGGLTTKLHLVCDGRGRPLSMMITAGNINDTTMMSAVLENIRVPRDGKGRPRTHPDRVLADKGYPSRANRAWLRDRRIAATIPER
DDQIAHRRKKPGRPIAFGDKQKERYKGRNVVERCFNRLKQWRGIAMRSDKLARNYRAAVSLAAALIWIKTDLR
KNPWSEPPDHGIGRSRGGLTTKLHLVCDGRGRPLSMMITAGNINDTTMMSAVLENIRVPRDGKGRPRTHPDRVLADKGYPSRANRAWLRDRRIAATIPER
DDQIAHRRKKPGRPIAFGDKQKERYKGRNVVERCFNRLKQWRGIAMRSDKLARNYRAAVSLAAALIWIKTDLR
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
886 bp | 295 aa | 49 | 934 | + | Yes |
Chemistry : DDE
ORF sequence :
MTRQEISDEVWAVMEPLMPTVTGRSRPWTDHRLAIEGMAWKYRTGAPWRDVPERFGKWNSIYKRFNRWAGDGTWQKLLTEVQKQADAAGEIDWVVSIDST
IARVHQHGATLARDTGGCAESQESVVRAADHGIGRSRGGLTTKLHLVCDGRGRPLSMMITAGNINDTTMMSAVLENIRVPRDGKGRPRTHPDRVLADKGY
PSRANRAWLRDRRIAATIPERDDQIAHRRKKPGRPIAFGDKQKERYKGRNVVERCFNRLKQWRGIAMRSDKLARNYRAAVSLAAALIWIKTDLR
IARVHQHGATLARDTGGCAESQESVVRAADHGIGRSRGGLTTKLHLVCDGRGRPLSMMITAGNINDTTMMSAVLENIRVPRDGKGRPRTHPDRVLADKGY
PSRANRAWLRDRRIAATIPERDDQIAHRRKKPGRPIAFGDKQKERYKGRNVVERCFNRLKQWRGIAMRSDKLARNYRAAVSLAAALIWIKTDLR
Blast result :
Comments
ISMcte1 is 67% aa similar to ISBli8.
ISMcte1 was found in a section of the draft genome of Micrococcus terreus strain 1AStreptC4R3 which was isolated on R2A-streptomycin agar from a faecal sample derived from a healthy farm horse. Two identical copies of ISMcte1 were found 7143 bp apart, with a section of transposon Tn21 inside them, which itself contained a class 1 integron (no gene cassettes) and a copy of IS1326. It is likely that the whole structure is a composite transposon although since the 4-bp direct repeats are identical at all four IS ends involved, this cannot be confirmed on the basis of the sequence alone. The mobilised region is 99% identical to In0 from Pseudomonas plasmid pVS1.
ISMcte1 was found in a section of the draft genome of Micrococcus terreus strain 1AStreptC4R3 which was isolated on R2A-streptomycin agar from a faecal sample derived from a healthy farm horse. Two identical copies of ISMcte1 were found 7143 bp apart, with a section of transposon Tn21 inside them, which itself contained a class 1 integron (no gene cassettes) and a copy of IS1326. It is likely that the whole structure is a composite transposon although since the 4-bp direct repeats are identical at all four IS ends involved, this cannot be confirmed on the basis of the sequence alone. The mobilised region is 99% identical to In0 from Pseudomonas plasmid pVS1.
References
1] Nicholas Coleman (2022) Direct submission.