ISMahy2

  • Family IS91
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_008740.1 ND Marinobacter hydrocarbonoclasticus
Marinobacter hydrocarbonoclasticus VT8
DNA section
IS Length : 2340 bp

Ends


oriIS : TAAACCGCTCCGCGGTAGCGGTGATACTGAGAATCTCCCCGGAATGGAGGTGATTGGAGGATACTTTTGAATGGCCCAAGTGGGCTTTTCAAAGAGGAGA II struct. : No
terIS : CCGCAGAGTACCTTTGCGGGCCTGAACCGCCATCCCGGTATCCGTTCAAAATTTACTTTACCCTTATACATTGTGTGCTGGCATTGGTGGCAGCGGCTTA II struct. : No

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
GCTGTT GAACGTCC AACCCA

DNA sequence

TAAACCGCTCCGCGGTAGCGGTGATACTGAGAATCTCCCCGGAATGGAGGTGATTGGAGGATACTTTTGAATGGCCCAAGTGGGCTTTTCAAAGAGGAGA
TGCCGCCATGACCCCCTTACGTCAGGCTATGATCCAGACCATGCGTCAGCATGGTTTTTCCCCGCAGACCCAAAAAAGTTATCTCTATGTAGTCGCGGAT
CTGGCTCGATATTATCGTCGATCCCCGGACCAGTTGGAGGTCGGGGATCTGCAGACCTACTTTAATTACCTCGTCCAGGAACGTTCCCTGGCGCCGGCCA
GTTGTCGGCTTTACCTGCATGGCATTCGGTTTTTGTATCTGCAGGTGCTGCATTGGGCAGACTTCGACGTGAACCTGGTGCTGCCCAAGCGACCACAGCG
CATTCCTGAGTTACTGACTCGTCAGGACGTTGCAAGGATAGTGGGAGCCACCAAGAACCCCAAGCATCGGGCCTTGCTGTTGATCACGTATGGCTGCGGC
TTGCGAGTGAGTGAAGCCGTGTCATTGCAAGTGAAGAACATCGACGGGGAGCGCCACCTGCTTCACATCCATCAGGGCAAGGGCGGTAAGGATCGCATGG
TGCCCCTGACTGACGTGCTACTCGATGTATTGCGAGCGTATTGGTGCCTTGGCCGCCCCGTACTCTGGCTGTTTCCGAGTGAAGTACTGGTTGGTCAGCA
CCTGACCGCCACCACGGCCCAGAAGGTTTATCGTCACGCCAAGCGACTCAGCGGTGTGCAAAGACGCGGAGGGATACACGCACTGCGCCACGCCTATGCG
ACCCATCAGCTGGAACATGGTGTACCGCTCAACGAGTTACAGAAATATCTTGGGCACAGTGATCTGCGCACCACGGAGCGCTACCTGCACTGGTTGCCGG
GAATATCCTCACAAAACCGTTCGCCTGCGGACTTGATCGCGGATCTGGGAGGTGCGTGATGGCCGGTTCAGTTACGGTCCAGTCGGCACTGACCCAGTTC
CTCTCGACCGACTCATTGGACCGCCACCGTCGCAAGGTGTGCAGCCGACTGATGGATTGCCGCACAGCCCGGATGGGCGGTATGGAAATGCGTTGTGATC
ACTGCAAAGCACGAACCGTGCATTACTACGGTTGCCGGGACCGGCATTGTCCGCAATGCCAGAGTCGGGCTAGCCAACAGTGGTGTGAGCGCCAGCGTCG
ATCGCTACTGCCGGTACCCTACTTCCACCTGGTGTTCACCGTGCCCCACGCCCTGAATGGCTGGGTTCAGGTGCACCCGGAAGTGGTTTACCGTCGCCTG
TTTGAGTCAGTTTGGAATACGCTCAGTCAGTTTGGCCATACAACCAAACACTTGCAGGGTGAGTTGGGCATGACAGCCGTGCTCCATACCTGGGGGCAGA
ATCTGAGTCGCCACGTTCACGTGCATTGCCTGATTCCGGGCGGGGTGCTCACAGAGGCCGGAGAATGGCATGAGGCTAAACATCAATATCTGTTCCCCGT
CAGGGCCTTGTCCCGCCGCTTTCGCGGCCGCATGGTATCGTCCCTCAGACGATCGGTCAGAACCGGCGAATTGCACAGACTCACGGAGCCTCAAGCCATC
GACGACGTACTCAAAAAAGTGATGCAGCAGGATTGGGTGGTTTACGCCCGCCCTTGTCTGAATCAGGCGGAGACGGTGGTGGATTACCTGGCTCGCTACA
CCCACCGCATTGCTATCAGTAACGGTCGTTTGTTGTCGCAGGACGGCGGCCGAATCATGATCCGCTATGTGGACTACCGGGAAGGAGGGCGCCAGAAGAG
CCTGCAACTGGAAGGGGCGGAATTTGTTCGACGGTTCCTCATGCATATCCTGCCCAAGGGCTTTATGCGGATTCGTCACTTTGGTTATCTGAGTAACCGA
ACCCGACGTCGCAAGCTGACGGCCATTCGTCAGGCCCTTCAAAAGCCACCCGAGGTCGATGTTGAAGTCAAGAATGGCTCGGAGTCCCAACGCAGCTGGC
CGTGCCCTCGATGCGAGGATGGTGTGGTTTATATGGTTCGGCAGATTCCCCGGTTTAAAACCGTGGGTAGAGTGACGGGTTAGCCGCTTTCCCTGGCGGC
TTCCCGTGAGCGCTACAGTTCCTGAGGAGCTGGGCTTCGACTCGGGCTGAGGCTGGCAATGAAAGAAACAGAGGGGTAGGCTGATCCAAATCACCCAGGG
ATGAGGCATTTTATGGGGACCATCAGGACGATACGCACTTCCGCAGAGTACCTTTGCGGGCCTGAACCGCCATCCCGGTATCCGTTCAAAATTTACTTTA
CCCTTATACATTGTGTGCTGGCATTGGTGGCAGCGGCTTA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
852 bp283 aa108959+No
ORF function : Accessory Gene
AG : IS91 integrase/resolvase

ORF sequence :

MTPLRQAMIQTMRQHGFSPQTQKSYLYVVADLARYYRRSPDQLEVGDLQTYFNYLVQERSLAPASCRLYLHGIRFLYLQVLHWADFDVNLVLPKRPQRIP
ELLTRQDVARIVGATKNPKHRALLLITYGCGLRVSEAVSLQVKNIDGERHLLHIHQGKGGKDRMVPLTDVLLDVLRAYWCLGRPVLWLFPSEVLVGQHLT
ATTAQKVYRHAKRLSGVQRRGGIHALRHAYATHQLEHGVPLNELQKYLGHSDLRTTERYLHWLPGISSQNRSPADLIADLGGA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1125 bp374 aa9592083+No
ORF function : Transposase
Chemistry : Y2

ORF sequence :

MAGSVTVQSALTQFLSTDSLDRHRRKVCSRLMDCRTARMGGMEMRCDHCKARTVHYYGCRDRHCPQCQSRASQQWCERQRRSLLPVPYFHLVFTVPHALN
GWVQVHPEVVYRRLFESVWNTLSQFGHTTKHLQGELGMTAVLHTWGQNLSRHVHVHCLIPGGVLTEAGEWHEAKHQYLFPVRALSRRFRGRMVSSLRRSV
RTGELHRLTEPQAIDDVLKKVMQQDWVVYARPCLNQAETVVDYLARYTHRIAISNGRLLSQDGGRIMIRYVDYREGGRQKSLQLEGAEFVRRFLMHILPK
GFMRIRHFGYLSNRTRRRKLTAIRQALQKPPEVDVEVKNGSESQRSWPCPRCEDGVVYMVRQIPRFKTVGRVTG

 

Blast result :
Comments
ISMahy2 is 72% aa (transposase) similar o ISHati3.
References
1] Sarah Sonbol (2020) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Edwards,K. and Richardson,P. (200-) Direct submission GenBank.