ISMlu14

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CP001628 ND Micrococcus luteus
Micrococcus luteus NCTC 2665
DNA section
IS Length : 2646 bp

Ends


IR Length : 29/38

IRL : TGTAAGCGGCCATGAAAAGCTGCCCATAGGTGGCCAGGGAATGACCCGCT
IRR : TGTCAAGGGCCATTGAGTTCTGCCCATAGGCGGCCAGTGTTTCTTCCCGC

Insertion site


Left flankDirect repeatRight flankDR Length
CGTCCACTCCCTGGCCGTGCAGGGGTGAGGGCGTC6

DNA sequence

TGTAAGCGGCCATGAAAAGCTGCCCATAGGTGGCCAGGGAATGACCCGCTGACGGCCATGAGGACTGCCCAGTGATGGCCACGGGAACTGCCCGGTGCTG
GCCATCTGGTTTGCCCGCGTTGCTCGTTGCCCGCCGCGTCGTGAGCCGGCGGGGGCCTCCTCCCCGGGTTGGATATCGGTGTCGAATCGAATCCAGCGAG
GAAGAGGCCCTGAATGAAGGATGCCGCAGAAACCATGAAAATACTCAGTGCCTATGACCTGACCAAGTCATTGCGGGGCGCCGCCGAGCTGGCCGGTTGC
TCCCATCACACCGTGGCCCGGCTGGTGCGGGCCCGCGACGCCGGCCAGACCCCTGGATCCGGAGCCAGCCGCCCGAAGGTCACCGATGTCTGGCTGCCCA
AGATCGAGGAATGGGTCGAGGCCTCCACGGGCAGGATCCGCGCCGATGTCGTGCACGCCAAGCTCACCGCAATGGGCTACACCGGCTCTGAGCGCTCCAC
CCGGCGGGCGGTGGCCGAGGTGAAGGCCGCCTGGCGGGCCGGGAAGCGCCGGGTGCACCGGCCCTGGATCACCGAGCCGGGCGCATGGCTGCAGTACGAC
TTCGGCGATGGGCCGGCGATCGACGGGGCCAAGACGGTGCTGTTCGTGGCCTGGCTGGCCTGGTCCCGCTACCGGATCGTCATCGCCCTGCGTGATAGGA
CGGCTCCGAGTGTGTTCGCCGCCCTGGACCGGTGCTTCCGGCTGATCGGGGGCGCGCCGACCTATGTGCTGACGGATAACGAGAAGACAGTGACGGTCTC
TCATGTGGCCGGGGTGCCGGTGCGTAACCAGGCCACCGTGGCGTTCGCTCGGCATTACGGCGTGGAGGTGCTGACCTGCCAGCCAGCGGATCCGGCCGCC
AAGGGCGGGGTGGAGAACGCGGTGAAGCTGGCCAAGGCCGACCTGGTCCCGAAGGACACCAACCTGCGGGACCAGTACGCCTCGTTCGCTGAGCTCGAGG
CCGCCTGCGCGGGGTTCATGGCCATGGTGAACTCCCGGGAGCACCGGGTGACCCGGCGCCGCCCGGACCACATGCTCGCCGAGGAGACCAGCGCGTTGCA
TCGGATCCCCGCCACGGCGCACACCGTGGCCTACGGGGTGGGACGGAAGGTGCCGGAGAACACGCCGATGGTCTCCTTCGAAAACGCCCAGTACTCGGTG
CCCGCGCACCTGCTCGGGGCCGAGGTGTTCGTGCGCCATCACGGCACCGGACCGGACTCGATGGTGGTGATCATGCACGCCGGTGCGCAGGGGCCGGTGG
AGGTCGCCCGGCACCGGGTGGCCCGGCCCGGGTCCCCGGCCATCGACGACGCGCACTTCCCCGGCCACGAGACGGACAAGGTGCCCAGGGACTACACCCC
GGTGCCCCGTTCGGCGGCGGAGGCCGAGTTCCTGGCCATCGGGGCCGGAGCGAGGACCTGGCTGGTGGAGGCCGCCGCGGCCGGCACCAGCCGGATCGGT
CAGAAGATGGCCGAGGCCGTCACCCTGGCCAAGCTCGCCGGCACCGACCAGGTCGACCGCGCCCTCGGGGTGGCCGCGGTGCACCAGCGCTTCGCCCACG
GCGACCTGGCCTCCCTGCTCACCGCCGCCGGCCACCGCACCGGGATGCACACCGCCACCGAGGAACGGTCCCTGACCCAGGGCACCGCCGGCTGGGCCGG
CCTCGGTACCTCCAACACCGAGGGGGCAGCCCGGTGAGCATCCACACCCCGAACACCGCCGCACCGGCGCTGCCGGCCGATTTGGAGGCGCTGTTGCGGC
AGCTGAAGATGCCCCACGCCCGCGGTATCGCCGCCGAGGTACTGGCCACCGCCCGGGCCCAACGCTGGGAGCCGGCCGAGGTCCTCAAGGCCCTGCTGGT
CGAGGAGACCACCGGTCGGGCCCGATCCATGCTCGCCGCCCGGCGCAAGGCCGCCGGGTTCCCCACCGGCAAGACCTTCACGACCTGGGATCCGGGCGCC
TCCTCGATCCCGGCCCCGACCCAGCAGTCCCTGCGCACCCTGGAATGGCTCTCGCGCAGAGAGAACCTCGTGGTCTGCGGGCCCTCCGGCACCGGCAAGA
CCTTCTTCCTCGAGGCCCTGGGCCAGCAGGCCGTGGAGGAGGGCAAGCGGGTGGCCTGGTTCACCCTGGAGGACCTCGGGGCGATGATCCGGGCCCACCG
GCCCGATGACACCATCACCAAAGCCGTCGCCCGAATCCTGCGCGCCGACCTGATCGTCATCGACGACATCGGCCTGCTGCCGGTCGCCGACGATGCCGCC
GAGGGCCTCTACCGCATCGTGGACGCCGCCTACGAGAAACGCTCCGTAGCGATCAGCTCGAACCTGCATCCGGCCGGCTTCGACGAGCTGATGCCCAAGA
CCCTGGCCACCGCCACCGTGGACCGGCTCCTGCACCACGCCCACGTCTGCCAGACCACCGGAGACTCCGTACGGATGACCCAGGCAATGGCCGGGAAGGG
AGTCATGCCACTGAACTGATCCCATCACGGTGGCCAGCACCACCACCCAAACGGGCAAACCAGCTGGCCACCAGTGGGCAGTTCTGATGGCCACCAGCGG
GAAGAAACACTGGCCGCCTATGGGCAGAACTCAATGGCCCTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1467 bp488 aa2711737+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGAAELAGCSHHTVARLVRARDAGQTPGSGASRPKVTDVWLPKIEEWVEASTGRIRADVVHAKLTAMGYTGSERSTRRAVAEVKAAWRAGKRRVHRPWI
TEPGAWLQYDFGDGPAIDGAKTVLFVAWLAWSRYRIVIALRDRTAPSVFAALDRCFRLIGGAPTYVLTDNEKTVTVSHVAGVPVRNQATVAFARHYGVEV
LTCQPADPAAKGGVENAVKLAKADLVPKDTNLRDQYASFAELEAACAGFMAMVNSREHRVTRRRPDHMLAEETSALHRIPATAHTVAYGVGRKVPENTPM
VSFENAQYSVPAHLLGAEVFVRHHGTGPDSMVVIMHAGAQGPVEVARHRVARPGSPAIDDAHFPGHETDKVPRDYTPVPRSAAEAEFLAIGAGARTWLVE
AAAAGTSRIGQKMAEAVTLAKLAGTDQVDRALGVAAVHQRFAHGDLASLLTAAGHRTGMHTATEERSLTQGTAGWAGLGTSNTEGAAR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
786 bp261 aa17342519+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSIHTPNTAAPALPADLEALLRQLKMPHARGIAAEVLATARAQRWEPAEVLKALLVEETTGRARSMLAARRKAAGFPTGKTFTTWDPGASSIPAPTQQSL
RTLEWLSRRENLVVCGPSGTGKTFFLEALGQQAVEEGKRVAWFTLEDLGAMIRAHRPDDTITKAVARILRADLIVIDDIGLLPVADDAAEGLYRIVDAAY
EKRSVAISSNLHPAGFDELMPKTLATATVDRLLHHAHVCQTTGDSVRMTQAMAGKGVMPLN

 

Blast result :Comments : 81% aa similar to IS1415.
Comments
ISMlu14 is 66% (transposase) aa similar to IS1415.
References
1] ISfinder annotation (2018)
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Lowry,S., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Lykidis,A., Young,M. and Greenblatt,C. (2009) Direct GenBank submission.