ISfinder

ISMph1

Family IS1595
Group ISNha5

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
FOPK01000001	ND	Methylobacterium phyllosphaerae	Methylobacterium phyllosphaerae CBMB27

DNA section

IS Length : 5381 bp

Ends

IR Length : 44/45

IRL : CGGTATTATGCAGCAACCTCACCTAAAAGCGTTGAGGTAGGAGGGTAGCG
IRR : CGGTATTATGCAGCAACCTCACCTAAAAGCGTTGAGGTAGGCGGGCAACC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CGAGCTTGGCGGCCG	GATAATCG	GGGCGGAGAGCGCCTTG	8

DNA sequence

CGGTATTATGCAGCAACCTCACCTAAAAGCGTTGAGGTAGGAGGGTAGCGTGATGAACGAGGAAGCCTACCTGCGTGAGCTATCGGACCTAGGCCGGCGG
CACGGCATCGGACTCACAGGCCGGACGCAGCTTTACCATCTTGAACCGGAGGATATGGCCTTCGACTATTCCGCCGACGCCGAAGGCTTTGTGCATCTCG
GCTTAGCGGGGGATGCGAACAGTGATGCCGGATCCGGGGCCAGGAGGGACACCCAAGGCTGTCTCCACGCCAGTACTGCGGCGGACATCTAGCAGCAGGA
TATCCGGATCAACGAGTAGTCCGATGAGTCGTTGCGACTCGGGCCTTGGCTGCATCACGGCTTTCGCGCGGTAAACCTCAAGCCGCGGAATGCCTTCCGA
CAAGGCCCGGCGGCTGAGTCCCCGCACATAAAACCGGTTGAACTCACCTTCGGCCAAAACCTCGGCTGCGTTGTAGGGCACCTTGGCTTGTGTGTAGCCG
CCGCGCGTCCGGCGCATCGTCGTGCGGTTCAACGCCGTGCCCATGCGCAGTCGAGCCGCTAACGTGTCGTCCGTTCCAGATTGTGCAGCCTCGCGAAGCC
AATCTGGCCAATCACCCTGTGCGCGCTGTGTCAGGTACGCGCTGAGATGGATGCGGTCGGAGGCCGCATCCATCTCGATCTCTTCGAGCATGAAGCGGCG
GGTTTCGGGATCAAGGTTTTCGTAACGCAGCCCCATCACCCAGCCCGACTCAGACCAACCCGCGCTTAGGTTGGCTCATCCTAGCTCACGTTGCTAGGCC
CAGCGTGGACCTCCGTAGGTCATGACCTCCAGCACTCTGGCCCTCCACACCTCCCACCCTCCGGAGGCGAATGGGAGAGAGGGCTTGCAAACGGACTTAG
AGTGCGTCAGGCTACAACTCGACCCGGAGTGTGTGGCGTGGCTGAGTCGTCGCCTTGGAGTTTCAGCTTGACCCGCGAGGACATCCTCGCGCGCAAGATG
AAGCGCGAGCAGATCGCTGAGGAGCTTGAGAAGCTTCAGCGTCAGCTTGAGGCTGAGGATAAGTGGTTCGAGGCCATCAAGCTCATCGTCCCCCACGACC
TACTCTCAAGCATCGAAGAGGCGGTTGCTGGAGCCAAGGAGGGACCTGAGAAGGTTTCTATCTGGCGCCAGGCGGTAGCGGACGCCTTAGCTGCGGCCGA
TTTCGGAATGCTCCCCAAGGATGTGGCGCTGTTCATCAAAGAACATGGTAGCGCGGAAGCAAAAGAGAGGATCGTCCGCAATCCAAACGGTGTCTACAAC
GCTCTGTATCGGATGCACAAAGACGGTCAGATCGTAAGGCATGGCGATAAATTCTATCGCATCGACTTGTACGAAGATCTCTTGGCGTCCGGAGAATTGG
ATGATGATGCAGACGATCGAGGTTCGTCTGGCGTCAACGCATTCATTCTCAGCCTCGTTGGGGAATGGGATCGCGTTTTGCCCCGTGATGTGATCGCCAA
GCTTCGCGAGCACGAGGAATATTCAAAGCGTGTCGAACGCAATCCTCAGTACGGATACAGCGCGCTGGCCCGATTGGTTCGGCAAGGCCGACTTGAGAAG
GACGGGGCTTATTACTCCCGTACCCTCAAAAAGAATGAGCCGTCCGACGTTCCCGCGTCGAACGGCTCAGATGCCGGGTCGGACGATGATGGGCGCCCAA
GGGCGCTTCGTCTCGTTTGACCTATTCGTTCCACCCCGCGTCCCGCTAGGCGCCAACAGAGGAGAACGTCGTGGCCGACAACCACGGGCTCAGAAGAGCC
TAACGGCTCAGGCCGGTGTTAGCGCACCGGCCTGAGCCAGAACCATCACACAATCACTACCCGGTTTGGACAGCGACCAAGAGCGACAGCCCCAACAGGT
CTAGAGTCGCACCTTTGGTCGCCTTGTTCAAGCCGGTCCCCTGCAATTCGCCTCAACGGCGTTAAGGACCGACTATGCCTGAGTTCCCTTGCGACCATCT
CACCGCCTGCCACATCCTCCATGCCGTTTTCGAACTCGGCTGGAGTCAGGGCAAGGCGTCCCATTACTTCTGCGTCAACGGTGGCACGGTCTCTAAGATC
GTGCGCGGCCTCTCCCATCACGGAGCGGCACCGGTCCCGTTCTTGTCCGGTGATGCGCCCGTGCCGGCGTAAATGATCCCGGCCGGCTCATCCGTAAGGG
TGATGCCGGCCTCCTCAAACGCACGCTGCATCGCCAGCATGTTGTTGGTGATCGGCGTGCGGATGCCGTTCTCAAACTGGCGGATTGTGCTCAGTCCGAT
ATTGGCGCGCTTGGATAGGTCGGTTTGCGACCAATCCAACCATCCTCTAGCGGCTCGGCAATGCGCTGGCGTCATCGTCACTGAGAGATAGGGAACAACT
TTTTTCGCGTCAAGTACCGAAAAGCGTTGACCTGATCCAAATCAACCCATAACGTTCACCTCAACGCAAAACGTTCGGGTGGCCGCGATGGCTCAGCACT
TCCTCCTCTCCAAAGCCGCCCGCTCCCTCTCGCTCGTTCAGGTCGCCCGCCTGTCCGACGACGCGGCTTGGGAGACGTTCAAGGCTATCCGCTGGGAGGC
GACTGAGGGGCAGCCGTTCTGCCCGCGCTGCGGCTGCCTCAAGCACTCGTTCATCGCTACCCGCAAGCTGTTCAAGTGCGCTGGGTGCCGGCACCAGTAC
AGCGTCACCAGCGGGACCATCTTCGCGTCGCGCAAGCTCCCGATCCGCGATCACCTCCTTGCCATCGCCATCTTCGCCAACGGCGCCAAGGGCCACAGCG
CGCTCCAGCTCTCCCGCGACCTGAACGTCCAGTACCGGACGGCGTTCGTGATGGCGCACAAGCTCCGTGAGGCTGTCGCGGCTGAGACGGCCGAGGCTAC
CGTGTCGGGCACCGTCGAGGTCGACGGCGCCTACTTCGGCGGCCACGTCCGCCCCGAGAACCGCAAAGAGGACCGGAAGGACCGCCGGCTTGCCGAGAAC
CAGACCGGCAAGCGCCGCGTCGTCGTCGTGATCCGCGAGCGCAACGGCAAGGCTCTCACCTTCGCCTTCCGCTCCGAGGCCGAGTCCGTGGAGACGATTG
AGGCCCGCGTGCTGCCCGGCTCCGTCGTTCATGCTGACGAGGCGAAGGGCTGGGACCGGCTGCACAAGAGCTTCACCACCCACCGCATCAACCATCAGGA
GAGCTACAGCGACGGCGAGGCCTGCACCAATCAGGCAGAGAGCTACTTTGCCCGGCTGCGGCGCTCTGAGGCTGGCGTGCATCACCGGATCGCCGGCCCG
TACCTCTCTGCCTACGCTCGTGAGATGGCTTGGCGCGAGAACTATCGCCGCGTGTCCAACGGCGGCCAGTACGCCATCATCGCGAGCGCTGCGCTGACCC
ACGCTCCGTCTCAGGTCTGGAAGGGCTACTGGCAGCGCCATCAGCTTGTGAAGGTGGGCGCCTGATGGCCCTCCCTCAGATCGTCAGCACCCTCACTGAG
AAGCGCAACGAGATCGAAGCGCACATCCAGGAGCTACGGCGCCAACTCTCCGCCGCGGAGCGCGATCTATCCAGCGTGAACGCTGTGCTCGCGCTCTATG
ACCTGTCGCCGTCTACTCAGACCCGCTTCCCAGCTTACGCCAACCTCAACCGGTTGTTCGCCTACGGAGAGATGTTCAAGCTGTGCAAGGCGGCGCTTGA
GGAGGCCGGGAAGCCACTCGACACGCGGGAGATCACGCGCGCCATCATCCGCGCAAAGGCCTGGGACGAGGGCGACGTGATGCTGCGGAAGGCCATAGCC
TACCGTCTTGTTCAAAACCTCTCGCGAGCCGCCATGCAAGGACGGATTGGGGATGCTGGCAAACGAAAAGGCGTGCGGGTATGGCGACTTCTCGAATAAA
GCCCGTCAAGCCGGTTTTGATGGTTGTGGTTGAACAACATTTATACGCAGCTGATTAAGTCTAGCAGTCTCTCCTTTGCATTCTACCTCTACTTTTATAA
AACTATCCTCTTCAACGATAACATTTGCGATCTTAACATTGAAAGACATATTCATTGTGATGGGGGCGTCGGGATACGCCCGGATCATACTGCCTAGAAT
TTCCGGCGGAGGCGCATTCGGTATGGTTGATGATTGGATAATGCTTGGGCTATCATGATCTGTCGGAAGGTATATTTTTGTTTTTAGACTGTCTCCAGCA
TACTCGATTGATGTCCTGATGCGTACTACTAGCTGTATCTGAGCAAGCGACGCGGGAGGTCGCGCGTTGACAACCATATCCGTATTGTACACACCGACTA
GTATCAGTTTGCCCGTGTTTTCTACTCTTACATCATCGCAGTATATTACATTGCCGTGCAACATCACGCCACCCGTGCGTCAAAAAAAGCGCCGGAAGAT
GTGTCTTCCTCTGCCTCGTCAACGACCTTTTTATTTGTGTTGTCGACAACCACCCTCAATTTGCGCGCCGAAGAACTATCCACGTCTGATGGAGAAGTCG
TTTTTACTTCATGCTCAACAATATCCACAAGTAATAGAGTTGGCTTCTTCCCAAGCGCCCAGGCCAGATCAGAAATGGACTTTGCTGTTAGATTGCTTCC
GAATTTAAGCTGTCTATAAATTGCGGATCTATTGATCCCGAGCTTCTGCCCAATGCTGTGGAATGTGGCGTGATCGCGTTTTTTGGCTTCATGAAAAGCA
GATTGTAATTTGCCTTTTATGTCGGCCAGAAAACCAGACTGCGCTTCCTCGCGTTCGTCAACATCAAGAAAGAACATAGGCTACACCGTTTTGTCTGACG
TAATTGACCTGATGGGCGGACCTAAAATTTTCAACCATTTGTCGATATGTTGGATACAAGCTGTACGTAATTATTCTAGATTTATAATCGGCGACCACTG
CGATAAATTCATCCATCTCAGAAAAAAAACCAAAAATTCGAAGCTCTGCTGTAGTTATTTCCCAGATGTTCTTCTCGCCTGGCCGCATTGGGTGAAATTC
TCTATCAAGTCTCATTTCACCCCTATTAATAAATGTTTTTAATATTCCAAAAAGTTGTAATCTTGGGCTTTCCTCTAGGCCATCGGGTGAGGCGGGGTTG
TTTCGATTGATGTTGTCCAGCCACGCGGCACACGGTGTTGTTGCGTAGAAACGCCTGGGGTTTGGCACCGGCTCCCGACGCGAGCCGCCGATTGGGTGCA
ATTCCTTCGCATCGACGAGCTCAGCCACTGTTGCCATGTAAGTCAACAAATTGGAAGGGGAAGTGCGGCGGCGCGCCGCTTGCCTCCTACCTAGAGCAAT
TTTCTACCTATGGTAATCGGTATGCCACATAGGTTGCCCGCCTACCTCAACGCTTTTAGGTGAGGTTGCTGCATAATACCG

Protein section

ORF number : 7

ORF 1

Length		Begin	End	Strand	Fusion ORF
240 bp	79 aa	53	292	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MNEEAYLRELSDLGRRHGIGLTGRTQLYHLEPEDMAFDYSADAEGFVHLGLAGDANSDAGSGARRDTQGCLHASTAADI

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
534 bp	177 aa	736	203	-	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MGLRYENLDPETRRFMLEEIEMDAASDRIHLSAYLTQRAQGDWPDWLREAAQSGTDDTLAARLRMGTALNRTTMRRTRGGYTQAKVPYNAAEVLAEGEFN
RFYVRGLSRRALSEGIPRLEVYRAKAVMQPRPESQRLIGLLVDPDILLLDVRRSTGVETALGVPPGPGSGITVRIPR

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
783 bp	260 aa	938	1720	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MAESSPWSFSLTREDILARKMKREQIAEELEKLQRQLEAEDKWFEAIKLIVPHDLLSSIEEAVAGAKEGPEKVSIWRQAVADALAAADFGMLPKDVALFI
KEHGSAEAKERIVRNPNGVYNALYRMHKDGQIVRHGDKFYRIDLYEDLLASGELDDDADDRGSSGVNAFILSLVGEWDRVLPRDVIAKLREHEEYSKRVE
RNPQYGYSALARLVRQGRLEKDGAYYSRTLKKNEPSDVPASNGSDAGSDDDGRPRALRLV

Blast result :

ORF 4

Length		Begin	End	Strand	Fusion ORF
198 bp	65 aa	1975	2172	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MPEFPCDHLTACHILHAVFELGWSQGKASHYFCVNGGTVSKIVRGLSHHGAAPVPFLSGDAPVPA

Blast result :

ORF 5

Length		Begin	End	Strand	Fusion ORF
222 bp	65 aa	2339	2118	-	No

ORF function : Passenger Gene
Annotation : Description : Transcriptional Regulator factor

ORF sequence :

MPEFPCDHLTACHILHAVFELGWSQGKASHYFCVNGGTVSKIVRGLSHHGAAPVPFLSGDAPVPA

Blast result :

ORF 6

Length		Begin	End	Strand	Fusion ORF
978 bp	325 aa	2488	3465	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAQHFLLSKAARSLSLVQVARLSDDAAWETFKAIRWEATEGQPFCPRCGCLKHSFIATRKLFKCAGCRHQYSVTSGTIFASRKLPIRDHLLAIAIFANGA
KGHSALQLSRDLNVQYRTAFVMAHKLREAVAAETAEATVSGTVEVDGAYFGGHVRPENRKEDRKDRRLAENQTGKRRVVVVIRERNGKALTFAFRSEAES
VETIEARVLPGSVVHADEAKGWDRLHKSFTTHRINHQESYSDGEACTNQAESYFARLRRSEAGVHHRIAGPYLSAYAREMAWRENYRRVSNGGQYAIIAS
AALTHAPSQVWKGYWQRHQLVKVGA

Blast result :

ORF 7

Length		Begin	End	Strand	Fusion ORF
435 bp	144 aa	3465	3899	+	No

ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MALPQIVSTLTEKRNEIEAHIQELRRQLSAAERDLSSVNAVLALYDLSPSTQTRFPAYANLNRLFAYGEMFKLCKAALEEAGKPLDTREITRAIIRAKAW
DEGDVMLRKAIAYRLVQNLSRAAMQGRIGDAGKRKGVRVWRLLE

Blast result :

Comments

ISMph1 is 92% (transposase) aa similar to ISMtsp22.

References

1] ISfinder annotation (2017)
2] Varghese,N. and Submissions,S. (2016) Direct GenBank submission.