ISOpi1

  • Family IS1595
  • Group ISPna2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
CCAX010000002 ND Oceanobacillus picturae
Oceanobacillus picturae S1
DNA section
IS Length : 1885 bp

Ends


IR Length : 22/25

IRL : GGCTGTTTTCGTATAGATTGTTGCTCAGGGAGACATACTGTATAATAGAA
IRR : GGCTGTTTTCTAAAAGATTGTTGCTATAATACAATGAATGTTATAGTATT

Insertion site


Left flankDirect repeatRight flankDR Length
TTGGTGTTACTACTTTTATTATTGAGGCAGGAAC8

DNA sequence

GGCTGTTTTCGTATAGATTGTTGCTCAGGGAGACATACTGTATAATAGAATAATTGATTGTACGGAGGGCTTCCAAAGATGTGGATAAATATGTACCGAG
ACTTTTCAGACCTATCAAAGAAAGAACAACTTCAATTCTTTAATGCTATAAAGGATGATTTATTTCCTGAACCAAAATCTTACATATCACAATTAGTCAG
TGAGGTTCGAGAGACACGCTTTTCTGAAGGATTAGCGTGTATTCATTGTGGAAGTATGTCTGTTAAGCGGAATGGTAAATATCGTTCACGCCAAAGGTAT
CTCTGTAAGGATTGTGGAAAATCCTTTAACGATATGACTGGAAGCCCATTATCAGGAACAAAGTATCCTCACAAGTGGATTAAATACTTTGAAATGATGG
TTGAAGGTTACACATTGCCTAAAATTTCAGAAGCTCTTGAAATACACATATCTACTGCTTTCTATTGGAGACACAAAATCTTAAACTCAATCCGTTCTCT
TGGACATAAAACTTTAAAAGGCATCATTGAAAGTGATGAAACTTTCTTTTTGGAGAGCAATAAAGGTAAAAAGATTATTTCACATAGAACTCCCCGCAAG
CGTGGTGGTGTAGCCAAAAAACGTGGTATCTCTAAGGAGCAGATATGCGTAGTAGTTGCACACGATAGAAACGGACAAATCTTATCACAGATGGCTGGTA
AAGGTCGCATTACAGCCGTTGAATTAGACAAGGTGTTAGGAAGTCATATTGACACCTCTGCTTTGTTATGCTCAGATACAGCTACGAATTATAAGAAGTT
TGCAACTATGAAAGGTCTTCAACACGAAGTTATCAATGTAAGTAAAAAAGTGTATAAAAGAAAAGGAATCTATCATATTCAACACGTCAACGGGTATCAC
AAACGCTTGAAGAAATGGATGGATAGATTTCAAGGTGTGGCAACTAAATATCTTGATAACTACCTTTTTTGGCATCGGTTTCTTGAACTTAATAAAAAGT
TTCCGTTTCAAGAACGCGTGAAAGAAATGCTTCTCGGTTCTTGTCAAAGGGTGAATTTTACCACAATTGAAGATATTAGAAAAACTGCTTAAAGATGATA
CGAACTAAAAAGAACCGTACAATTAATGAATATGTACGGCTAAATAATGCTTTTAATTTATTTTTCTTATGATGTCATTTTCTACACTATAAACACCACA
ACCTTCAATAGATTCAAGATATCCGTTTAGAAGGTAAACACAAGTGTTTTCATCTATACCATAATTTATATGGTTATTAAATTTATTGACTGCGTTTCTT
AAATGAGTTTCATCATTCCATTGTGAAAAGTGTACAGCAATCAATACATCAGATATTAAACCCAATCCCCTTCTGTATTGAAATTCGTTTTGTTCGTTAT
CTTTAGGAGAAATAATACAGTGTTCAGGACTTATTAAAGCACCTGCCGAAAACCCAGCAATAGGGATACCTAGTTCATAGATTTCTTTTATGGTGTTAGA
AATAGCGGTCTCAACAATGTAGTCAGCATATAAATTCGTATTCCCTCCGCCAATAATGAGTCCTGAACTATTTTTTAAACAAGTTATAACCTTTTCAATA
GGTGTTGAAGGAAGGGGTAAATAATAGAATTCATTTAATCCTAATTCCTCCAATGCTTGTGTATATTTGGGCATATATTGTTCCCAGCCGTCCCTTTCTA
TAAATAAAATTGAGACGGGAGCATTTGTTGTTGATGCTTTTATTACAAACTGTTTTGTCATATTTAATGTGAATGGAGGACCGCCTCCAAAAAGAAATAA
ATGTTTATCCATCATATCACCTTCGTTTTTTATAGAATACTATAACATTCATTGTATTATAGCAACAATCTTTTAGAAAACAGCC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1014 bp337 aa791092+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MWINMYRDFSDLSKKEQLQFFNAIKDDLFPEPKSYISQLVSEVRETRFSEGLACIHCGSMSVKRNGKYRSRQRYLCKDCGKSFNDMTGSPLSGTKYPHKW
IKYFEMMVEGYTLPKISEALEIHISTAFYWRHKILNSIRSLGHKTLKGIIESDETFFLESNKGKKIISHRTPRKRGGVAKKRGISKEQICVVVAHDRNGQ
ILSQMAGKGRITAVELDKVLGSHIDTSALLCSDTATNYKKFATMKGLQHEVINVSKKVYKRKGIYHIQHVNGYHKRLKKWMDRFQGVATKYLDNYLFWHR
FLELNKKFPFQERVKEMLLGSCQRVNFTTIEDIRKTA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
660 bp219 aa18121153-No
ORF function : Passenger Gene
Annotation : CyanophycinaseDescription :

ORF sequence :

MDKHLFLFGGGPPFTLNMTKQFVIKASTTNAPVSILFIERDGWEQYMPKYTQALEELGLNEFYYLPLPSTPIEKVITCLKNSSGLIIGGGNTNLYADYIV
ETAISNTIKEIYELGIPIAGFSAGALISPEHCIISPKDNEQNEFQYRRGLGLISDVLIAVHFSQWNDETHLRNAVNKFNNHINYGIDENTCVYLLNGYLE
SIEGCGVYSVENDIIRKIN

 

Blast result :
Comments
ISOpi1 is 87% aa similar to ISBci1.
References
1] ISfinder annotation (2016)
2] Croce,O., Lagier,J.C. and Raoult,D. (2014) Direct GenBank submission.