ISA0963-1

  • Family IS481
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AE001093 ND Archaeoglobus fulgidus
Archaeoglobus fulgidus
DNA section
IS Length : 963 bp

Ends


IR Length : 20/24

IRL : TGTAGTATCCCAAAATTATTTCACAACCCTAAACCCAACTCTTGTGAGAA
IRR : TGTGATATCCCGAAATAATTTCACTCCTCAAACAACCACCTCCCGTACTC

Insertion site


Left flankDirect repeatRight flankDR Length
AAAGATGAAAGAAAAACTTCTTTTGACAAAAAGTT15

DNA sequence

TGTAGTATCCCAAAATTATTTCACAACCCTAAACCCAACTCTTGTGAGAAAACTAACGAACAAAAAGATCAAGTGGATCATCAAACAGCTCGAAAAGGGA
ACACCAGTAAAGGAAATAGCTGCTGTGATGAGAGTTACACCAAGAAGAATCTACCAGCTTAAAAAGCAATACGAGGAAACTGGCCAGATCCCAGAACTAA
AACAAGCAGGAAGAAAGCCCAAACCAATTGACGAGAAAACAGAGCAAATCATCCTGCAAGCCTACAAAAAATACAGGGTAAGTCCAGTACCTCTTTAAAA
GCTGATAGAAAGAAATTACAGAATCCACATCCCCCACAACACCATATACAAAGTTTTACTCAAACACGGTTTGGTGGAGGAGAACATGAACAAGAAGAAA
AGGAGGAAGTGGGTTCGCTATGAAAGGACTCATTCAATGTCTTTATGGCAGGGAGACTGGAAAAAGCTTGGAGAGAAATGGATTATAGCCTTCATGGACG
ATGCTTCCCGCTTCATCACCTGCTATGGAGTATTTGACTCTGCTACTACTGAGAACACAATCAAGGTGTTCAAGAAAGGATTCGCTGAGTATGGCATTCC
GGATGAAATCCTCACAGACCATGGTACCCAATTTGTTGCAGCGAAAAGCAGAGAGAAGGCTAGGCACAGATTCAGAGAGTTTTTGGCCGAGAATGGAGTA
AGGCACATTCTTGCGAGAGTGAACCATCCACAAACGAATGGGAAGATAGAGAGGTTCTTTGGGCTGATGGAGCAGAAGCTCCATCTGTTTGATTCTTTGG
ATGAGTTCATTTATTGGTACAATTACGTTAAGCCTCACATGAGCCTGAATTTTGATGAACTGGAGACTCCTTACCAAGCTTTTCTGAGGAAGCTACCTGC
TGAAAGGGTGTTTGAGTACGGGAGGTGGTTGTTTGAGGAGTGAAATTATTTCGGGATATCACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
900 bp299 aa44943+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VRKLTNKKIKWIIKQLEKGTPVKEIAAVMRVTPRRIYQLKKQYEETGQIPELKQAGRKPKPIDEKTEQIILQAYKKYRVSPVPLKLIERNYRIHIPHNTI
YKVLLKHGLVEENMNKKKRRKWVRYERTHSMSLWQGDWKKLGEKWIIAFMDDASRFITCYGVFDSATTENTIKVFKKGFAEYGIPDEILTDHGTQFVAAK
SREKARHRFREFLAENGVRHILARVNHPQTNGKIERFFGLMEQKLHLFDSLDEFIYWYNYVKPHMSLNFDELETPYQAFLRKLPAERVFEYGRWLFEE

 

Blast result :
Comments
At position 85, there is a codon STOP (TAA). The authors state that there is an authentic frameshift at this position. Others members of this family carry GAA codon (E). Assuming a mutation at this position the extended reading frame shows homology over its enrtire length to others members of IS481 family. ISA0963-1 is 96% aa similar to ISA0963-2, ISA0963-3,ISA0963-4 and ISA0963-5, 95% to ISA0963-6 and 43% to ISBst1.
References
1] Klenk,H.P., Clayton,R.A., Tomb,J., White,O., Nelson,K.E., Ketchum,K.A., Dodson,R.J., Gwinn,M., Hickey,E.K., Peterson,J.D., Richardson,D.L., Kerlavage,A.R., Graham,D.E., Kyrpides,N.C., Fleischmann,R.D., Quackenbush,J., Lee,N.H., Sutton,G.G., Gill,S., Kirkness,E.F., Dougherty,B.A., McKenney,K., Adams,M.D., Loftus,B., Peterson,S., Reich,C.I., McNeil,L.K., Badger,J.H., Glodek,A., Zhou,L., Overbeek,R., Gocayne,J.D., Weidman,J.F., McDonald,L., Utterback,T., Cotton,M.D., Spriggs,T., Artiach,P., Kaine,B.P.,Sykes,S.M., Sadow,P.W., D'Andrea,K.P., Bowman,C., Fujii,C., Garland,S.A., Mason,T.M., Olsen,G.J., Fraser,C.M., Smith,H.O.,Woese,C.R. and Venter,J.C. (1997) Nature 390 (6658), 364-370