ISMeme1

  • Family IS5
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
JRHO01000014 ND Methanococcoides methylutens
Methanococcoides methylutens DSM 2657
DNA section
IS Length : 1000 bp

Ends


IR Length : 16/17

IRL : GGCTCTGTAGAAATCCTATTTCTAATCCTACTGAGTCAGTACACTTTTTA
IRR : GGCTTTGTAGAAATCCTCAACCAGACTAAACATACAATCTTGACATATCT

Insertion site


Left flankDirect repeatRight flankDR Length
AAAAGAAACCTCAATTCCAAAAGTTCAT8

DNA sequence

GGCTCTGTAGAAATCCTATTTCTAATCCTACTGAGTCAGTACACTTTTTATAAGAATAAGTCCCCTGTGATTGTTTGCAACCAAAAACACAAGGGGATGA
TTGTGTTTTGTCTAACAAGTACTTAAATTTTATTGATATAGCATTGCAAGTAACACGGTATTCACACCTGCCACTCTACAGTTGCAAATATTCCAAACAA
ACATACACACAATACCAGCTATTGACGTTGGTTTTGTTTAAGGAATATTTAGGAGAAGATTATAGGGATTTTGTTGATCTTGTTGAATTGATGAGTTCTA
TCCAGACTAAACTTGGATTAACCAAAGTGCCTCATTTTACTACACTTCAAAAATTTGTTACTCGGATCTATTCAGCAATACTCGATCGTATTTTCAAGAA
GACTTTAGACTTATTTTACAAGAATGGAGAGTCAGTTGAAGTTACTGGTATCGATTTAACTGGTTTTACAAGTGGGTATTGTAGCAACTACTATTCCTGG
AGAATCAAAAAGTGGAGACGAAGCTATGTTAAAACCAGTATTTCAGTTGATGTTCACAAATTTGTGATCACGGGATACAAGATTTCTGGAAAACCTGTTC
ATGATGCAAAGCATGCAAAGACATTGCTATCGCAATGTCATCGTAACAGGAAATCAAGATATTATGTAATGGATAAAGCGTATGATTCTGAAGGCAACCA
TAAATTGACTAGAGAAAAACTCAGATCAATAGCAATTGTTCCACTGAGACAGCGTGAAAGAAAGAGGATAAAAGGACATTATAGGAAGAAAATGTTACGA
GAATTTGATGATGAAATTTACTCGCTGCGAAATTTGAGTGAAACAATGTTCTCTCTATTGAAAAGAAAATATGGAGAAAATCTGAGAGCTAGGAAATATA
GAAACCAAGTAAAAGAAGTAAAATTCAAGGTGTTAATACACAATCTTGACAGATATGTCAAGATTGTATGTTTAGTCTGGTTGAGGATTTCTACAAAGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
893 bp297 aa1081000+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSNKYLNFIDIALQVTRYSHLPLYSCKYSKQTYTQYQLLTLVLFKEYLGEDYRDFVDLVELMSSIQTKLGLTKVPHFTTLQKFVTRIYSAILDRIFKKTL
DLFYKNGESVEVTGIDLTGFTSGYCSNYYSWRIKKWRRSYVKTSISVDVHKFVITGYKISGKPVHDAKHAKTLLSQCHRNRKSRYYVMDKAYDSEGNHKL
TREKLRSIAIVPLRQRERKRIKGHYRKKMLREFDDEIYSLRNLSETMFSLLKRKYGENLRARKYRNQVKEVKFKVLIHNLDRYVKIVCLVWLRISTK

 

Blast result :Comments : The STOP codon is after the end of the IS, the end of the transposase was in silico deleted.
Comments
ISMeme1 is 80% aa similar to ISMev1.
References
1] ISfinder annotation (2016)
2] Guan,Y., Ngugi,D.K., Blom,J., Ali,S., Ferry,J.G. and Stingl,U. (2014) Genome Announc 2 (6).