ISMno21
- Family IS630
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
CP001350 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 plasmid pMNOD01 |
DNA section
IS Length : 1162 bp
Ends
IR Length : 18/24
IRL : CCAAGCCTCGTTGATCGAGACTCACGCAGGCTGGCGGTGGGGTCCTCAAC
IRR : CCAACTCTCATTGATCAGAACCCATGGGCCCAGGCGCGCAAGCCGATGGA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CCTCTTCTAA | TA | TAAGTGTCTC | 2 |
TCCTCAACTA | TA | TTAGCCTCAT | 2 |
TTGGCCTTCA | TA | TCCCAACCCG | 2 |
DNA sequence
CCAAGCCTCGTTGATCGAGACTCACGCAGGCTGGCGGTGGGGTCCTCAACTATGATTCTGTCCTCGGGTTTGAAGCGCGAGGAGGCTGGATTGGCCGCTC
CCGTTCCGCTGCGGTCGGACTTTGACGCCGCGGCTCTGCGTCGTTTGGCCAGAGCGTCGCGCGATCCGGCTCAAACCCGCCGTCTGCTGGCGCTGTCGGC
GATCGACGCGGGCGGGTCACGCACGCAGGCCGCCGCGCTCGGCGGGGTCGGGCTGCAGACGGGGCGCGACTGGGTGCTGGCCTTCAACGCAGACGGCCCG
GACGGGCTGATCGACGCCAAGGCCCCCGGTGCGCGCCCGCGCCTGAACGCCCGGCAGCGGGAGGCGCTGCGCGCCTTGGTCGAGCAGGAGCCCATGCCCG
CCATCCATGGTGTCGTGCGCTGGCGGCTGGTCGATCTGGTCCAGATCCTGTTCGAGGACCACGGCGTCTCGGTCTCCCAACAGACGCTGAGCCGGGTTCA
GCGCGGCATGGGCTATCGGAAGCTCTCGGCCCGTCCCCGCCATCACGCCCAGGATCCGCAGGCCACCGCCGCGTTTAAAAAGCCTTCCCCGCCCGCTGGG
CGCAGATCGCGCAGGCCACAGGCGGCAAGCCGATAGAGATCTGGTTTGCCGACGAGGCCCGCGTCGGCCAGACGAACACCATCCCGCGGCGCTGGGCGCG
CCGCGGCACACGCCCCTCGGCTCCGAAGGACCAGCGCACGGCCTCCGCCTCCCTGTTCGGGGCGATCTGCCCGGCCCGTGGCACGGGAGCCGGGCTCGTC
ATGCCCCGCTGCACCACCGAAGCGATGAACCTGCACCTGGCTGAGATCGCCAAGGCCGTCGCGCCGGGCGCGCACGCCGTCCGGCTGCTCGATCAGGCCG
GCTGGCACACGACCAGACAACTCGTGGTCCCTGCCAACATCACCCTGCTGCCGCTGCCAGCGCGCTCGCCTGAACTCAACCCAGTGGAGAACATCTGGCA
GTTTATACGCGAGAACTGGCTCGGCAACCGGATCTTCGCCTCCTCCGACGCCATCCTCGATCACTGCTGCCATGCCTGGAACAGGCTCATCGACCAGCCC
TGGCGCATCATCTCCATCGGCTTGCGCGCCTGGGCCCATGGGTTCTGATCAATGAGAGTTGG
CCGTTCCGCTGCGGTCGGACTTTGACGCCGCGGCTCTGCGTCGTTTGGCCAGAGCGTCGCGCGATCCGGCTCAAACCCGCCGTCTGCTGGCGCTGTCGGC
GATCGACGCGGGCGGGTCACGCACGCAGGCCGCCGCGCTCGGCGGGGTCGGGCTGCAGACGGGGCGCGACTGGGTGCTGGCCTTCAACGCAGACGGCCCG
GACGGGCTGATCGACGCCAAGGCCCCCGGTGCGCGCCCGCGCCTGAACGCCCGGCAGCGGGAGGCGCTGCGCGCCTTGGTCGAGCAGGAGCCCATGCCCG
CCATCCATGGTGTCGTGCGCTGGCGGCTGGTCGATCTGGTCCAGATCCTGTTCGAGGACCACGGCGTCTCGGTCTCCCAACAGACGCTGAGCCGGGTTCA
GCGCGGCATGGGCTATCGGAAGCTCTCGGCCCGTCCCCGCCATCACGCCCAGGATCCGCAGGCCACCGCCGCGTTTAAAAAGCCTTCCCCGCCCGCTGGG
CGCAGATCGCGCAGGCCACAGGCGGCAAGCCGATAGAGATCTGGTTTGCCGACGAGGCCCGCGTCGGCCAGACGAACACCATCCCGCGGCGCTGGGCGCG
CCGCGGCACACGCCCCTCGGCTCCGAAGGACCAGCGCACGGCCTCCGCCTCCCTGTTCGGGGCGATCTGCCCGGCCCGTGGCACGGGAGCCGGGCTCGTC
ATGCCCCGCTGCACCACCGAAGCGATGAACCTGCACCTGGCTGAGATCGCCAAGGCCGTCGCGCCGGGCGCGCACGCCGTCCGGCTGCTCGATCAGGCCG
GCTGGCACACGACCAGACAACTCGTGGTCCCTGCCAACATCACCCTGCTGCCGCTGCCAGCGCGCTCGCCTGAACTCAACCCAGTGGAGAACATCTGGCA
GTTTATACGCGAGAACTGGCTCGGCAACCGGATCTTCGCCTCCTCCGACGCCATCCTCGATCACTGCTGCCATGCCTGGAACAGGCTCATCGACCAGCCC
TGGCGCATCATCTCCATCGGCTTGCGCGCCTGGGCCCATGGGTTCTGATCAATGAGAGTTGG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
585 bp | 194 aa | 52 | 636 | + | No |
Description : First part of the transposase
ORF sequence :
MILSSGLKREEAGLAAPVPLRSDFDAAALRRLARASRDPAQTRRLLALSAIDAGGSRTQAAALGGVGLQTGRDWVLAFNADGPDGLIDAKAPGARPRLNA
RQREALRALVEQEPMPAIHGVVRWRLVDLVQILFEDHGVSVSQQTLSRVQRGMGYRKLSARPRHHAQDPQATAAFKKPSPPAGRRSRRPQAASR
RQREALRALVEQEPMPAIHGVVRWRLVDLVQILFEDHGVSVSQQTLSRVQRGMGYRKLSARPRHHAQDPQATAAFKKPSPPAGRRSRRPQAASR
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
570 bp | 189 aa | 579 | 1148 | + | No |
Description : Second part of the transposase
ORF sequence :
KAFPARWAQIAQATGGKPIEIWFADEARVGQTNTIPRRWARRGTRPSAPKDQRTASASLFGAICPARGTGAGLVMPRCTTEAMNLHLAEIAKAVAPGAHA
VRLLDQAGWHTTRQLVVPANITLLPLPARSPELNPVENIWQFIRENWLGNRIFASSDAILDHCCHAWNRLIDQPWRIISIGLRAWAHGF
VRLLDQAGWHTTRQLVVPANITLLPLPARSPELNPVENIWQFIRENWLGNRIFASSDAILDHCCHAWNRLIDQPWRIISIGLRAWAHGF
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1097 bp | 365 aa | 52 | 1148 | + | Yes |
Chemistry : DDE
ORF sequence :
MILSSGLKREEAGLAAPVPLRSDFDAAALRRLARASRDPAQTRRLLALSAIDAGGSRTQAAALGGVGLQTGRDWVLAFNADGPDGLIDAKAPGARPRLNA
RQREALRALVEQEPMPAIHGVVRWRLVDLVQILFEDHGVSVSQQTLSRVQRGMGYRKLSARPRHHAQDPQATAAFKKAFPARWAQIAQATGGKPIEIWFA
DEARVGQTNTIPRRWARRGTRPSAPKDQRTASASLFGAICPARGTGAGLVMPRCTTEAMNLHLAEIAKAVAPGAHAVRLLDQAGWHTTRQLVVPANITLL
PLPARSPELNPVENIWQFIRENWLGNRIFASSDAILDHCCHAWNRLIDQPWRIISIGLRAWAHGF
RQREALRALVEQEPMPAIHGVVRWRLVDLVQILFEDHGVSVSQQTLSRVQRGMGYRKLSARPRHHAQDPQATAAFKKAFPARWAQIAQATGGKPIEIWFA
DEARVGQTNTIPRRWARRGTRPSAPKDQRTASASLFGAICPARGTGAGLVMPRCTTEAMNLHLAEIAKAVAPGAHAVRLLDQAGWHTTRQLVVPANITLL
PLPARSPELNPVENIWQFIRENWLGNRIFASSDAILDHCCHAWNRLIDQPWRIISIGLRAWAHGF
Blast result :
Comments
ISMno21 is 71% aa similar to ISGdi4.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.