ISMyma8
- Family IS3
- Group IS407
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Mycobacterium marinum | Mycobacterium marinum 324-958 |
DNA section
IS Length : 1247 bp
Ends
IR Length : 32/41
IRL : TGAGGTGGGCCCTCTGTAGTGGTCCAGTCGGTGTGGGGCTTTGTTGCCCG
IRR : TGAGGGGGTCCCGATTGGTGGTCCAGTCGCTATGCGGCTTGTGGTTGGTG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTGACGAGTG | CATG | GCTCTCGTCG | 4 |
| TCAACTGGGA | GCTG | GTCTGGTGCC | 4 |
| GCGATGACCG | CCAT | ACCCAGCCCG | 4 |
| CGGCTTGGGG | GTGC | TGCCTGATCA | 4 |
| CCCATGAGTT | CCCG | TACTGCTTGT | 4 |
| GATCTCGGCT | GTGG | CGCTGGCCTC | 4 |
DNA sequence
TGAGGTGGGCCCTCTGTAGTGGTCCAGTCGGTGTGGGGCTTTGTTGCCCGGTGTTCGGGCAGGAAGAATCACCAACGACATGGCATCGCACAAGCGTCGG
CGGCACACGCCGGATCAGATCATCCGCAAGCTCGCTGAGGGCAACAAACTCCTCGGAACCGGCCAGGAGTTGGCCGAAGTGTGCCGACATCTGGAAGTCG
CGGAATCGACCTGGCATCGATGGGTGGCCCAGTACGGCGGCATGAAAGCTAACGACGCCAAACGGCTCAAAGAGCTTGAGGCCGAGAACGCCCGGCTGAA
GAGGATGGTCGCCAACCAGGCGCTCGACATCGACATGCTCAAGGAGATTCAGGCGGGAAACTTCTGACCCCGAACCGCAAACGCCGCGCAGCCTCCATGC
TGCGCGAGCGGTTCGGGGTCTCCCAGCGTCGGGCCTGCACCGTGGTCGGCATCCATCGCTCCACGATGCGCTTGCACCCCACGCCGGCTGCCGCTGACGA
CGAGCAGCTGCGTGCCTGGCTGCGCCGGTTCTCCACCGAGCGGCCGCGGTGGGGCTGGCGGCGGGCCGCGGTGATGGCGCGTCGAGCTGGCTGGACGGTC
AACAACAAGCGCATCCGCCGGCTGTGGCGCGAAGAGGGCCTGCGCGTCCCTCACCGCCGTAGAAAAAAACGCATGACCGGCATCGGCGTCACCGTCGGCG
CGATGTCGCCGATCCGGCCCAACGCCATCTGGGCGATGGATTTCCAATTTGACACCACCGCCGATGGCCGAACGCTGAAGATGCTCAACGTCATCGACGA
ATTCACCCGCCAAGCCCTGGCCATTGAGGTCGATCGATCCATCACCGCCGACGGCGTTGTCGCCGTGCTGGACCGGCTGGCTACAGCGCGCGGCGCCCCG
GCCTACGTGCGTTTCGACAACGGGCCTGAGTTCGTGGCCCAGGCCGTCAACGACTGGTGCCGATTCACCGGCGCCGGGTCGCTGTTCATCGATCCCGGAT
CGCCCTGGCAGAACGCCTGGGTCGAATCGTTCAACGGCCGGTTCCGCGACGAACTGCTCAACGCGTGGCGCTTCGACTCCCTGCTCGAAGCCCGAGTCAT
CATCGAAGACTGGAGGACCGACTACAACGCCAACAGACCCCACTCCGCCCACGGCGAACTCACTCCACACCAATTCGCCCTACAGTGGACCGCGACCCAC
CAACCACAAGCCGCATAGCGACTGGACCACCAATCGGGACCCCCTCA
CGGCACACGCCGGATCAGATCATCCGCAAGCTCGCTGAGGGCAACAAACTCCTCGGAACCGGCCAGGAGTTGGCCGAAGTGTGCCGACATCTGGAAGTCG
CGGAATCGACCTGGCATCGATGGGTGGCCCAGTACGGCGGCATGAAAGCTAACGACGCCAAACGGCTCAAAGAGCTTGAGGCCGAGAACGCCCGGCTGAA
GAGGATGGTCGCCAACCAGGCGCTCGACATCGACATGCTCAAGGAGATTCAGGCGGGAAACTTCTGACCCCGAACCGCAAACGCCGCGCAGCCTCCATGC
TGCGCGAGCGGTTCGGGGTCTCCCAGCGTCGGGCCTGCACCGTGGTCGGCATCCATCGCTCCACGATGCGCTTGCACCCCACGCCGGCTGCCGCTGACGA
CGAGCAGCTGCGTGCCTGGCTGCGCCGGTTCTCCACCGAGCGGCCGCGGTGGGGCTGGCGGCGGGCCGCGGTGATGGCGCGTCGAGCTGGCTGGACGGTC
AACAACAAGCGCATCCGCCGGCTGTGGCGCGAAGAGGGCCTGCGCGTCCCTCACCGCCGTAGAAAAAAACGCATGACCGGCATCGGCGTCACCGTCGGCG
CGATGTCGCCGATCCGGCCCAACGCCATCTGGGCGATGGATTTCCAATTTGACACCACCGCCGATGGCCGAACGCTGAAGATGCTCAACGTCATCGACGA
ATTCACCCGCCAAGCCCTGGCCATTGAGGTCGATCGATCCATCACCGCCGACGGCGTTGTCGCCGTGCTGGACCGGCTGGCTACAGCGCGCGGCGCCCCG
GCCTACGTGCGTTTCGACAACGGGCCTGAGTTCGTGGCCCAGGCCGTCAACGACTGGTGCCGATTCACCGGCGCCGGGTCGCTGTTCATCGATCCCGGAT
CGCCCTGGCAGAACGCCTGGGTCGAATCGTTCAACGGCCGGTTCCGCGACGAACTGCTCAACGCGTGGCGCTTCGACTCCCTGCTCGAAGCCCGAGTCAT
CATCGAAGACTGGAGGACCGACTACAACGCCAACAGACCCCACTCCGCCCACGGCGAACTCACTCCACACCAATTCGCCCTACAGTGGACCGCGACCCAC
CAACCACAAGCCGCATAGCGACTGGACCACCAATCGGGACCCCCTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 288 bp | 95 aa | 80 | 367 | + | No |
Description : First part of the transposase
ORF sequence :
MASHKRRRHTPDQIIRKLAEGNKLLGTGQELAEVCRHLEVAESTWHRWVAQYGGMKANDAKRLKELEAENARLKRMVANQALDIDMLKEIQAGNF
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 939 bp | 312 aa | 280 | 1218 | + | No |
Description : Second part of the transposase
ORF sequence :
GRERPAEEDGRQPGARHRHAQGDSGGKLLTPNRKRRAASMLRERFGVSQRRACTVVGIHRSTMRLHPTPAAADDEQLRAWLRRFSTERPRWGWRRAAVMA
RRAGWTVNNKRIRRLWREEGLRVPHRRRKKRMTGIGVTVGAMSPIRPNAIWAMDFQFDTTADGRTLKMLNVIDEFTRQALAIEVDRSITADGVVAVLDRL
ATARGAPAYVRFDNGPEFVAQAVNDWCRFTGAGSLFIDPGSPWQNAWVESFNGRFRDELLNAWRFDSLLEARVIIEDWRTDYNANRPHSAHGELTPHQFA
LQWTATHQPQAA
RRAGWTVNNKRIRRLWREEGLRVPHRRRKKRMTGIGVTVGAMSPIRPNAIWAMDFQFDTTADGRTLKMLNVIDEFTRQALAIEVDRSITADGVVAVLDRL
ATARGAPAYVRFDNGPEFVAQAVNDWCRFTGAGSLFIDPGSPWQNAWVESFNGRFRDELLNAWRFDSLLEARVIIEDWRTDYNANRPHSAHGELTPHQFA
LQWTATHQPQAA
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1139 bp | 379 aa | 80 | 1218 | + | Yes |
Chemistry : DDE
ORF sequence :
MASHKRRRHTPDQIIRKLAEGNKLLGTGQELAEVCRHLEVAESTWHRWVAQYGGMKANDAKRLKELEAENARLKRMVANQALDIDMLKEIQAGKLLTPNR
KRRAASMLRERFGVSQRRACTVVGIHRSTMRLHPTPAAADDEQLRAWLRRFSTERPRWGWRRAAVMARRAGWTVNNKRIRRLWREEGLRVPHRRRKKRMT
GIGVTVGAMSPIRPNAIWAMDFQFDTTADGRTLKMLNVIDEFTRQALAIEVDRSITADGVVAVLDRLATARGAPAYVRFDNGPEFVAQAVNDWCRFTGAG
SLFIDPGSPWQNAWVESFNGRFRDELLNAWRFDSLLEARVIIEDWRTDYNANRPHSAHGELTPHQFALQWTATHQPQAA
KRRAASMLRERFGVSQRRACTVVGIHRSTMRLHPTPAAADDEQLRAWLRRFSTERPRWGWRRAAVMARRAGWTVNNKRIRRLWREEGLRVPHRRRKKRMT
GIGVTVGAMSPIRPNAIWAMDFQFDTTADGRTLKMLNVIDEFTRQALAIEVDRSITADGVVAVLDRLATARGAPAYVRFDNGPEFVAQAVNDWCRFTGAG
SLFIDPGSPWQNAWVESFNGRFRDELLNAWRFDSLLEARVIIEDWRTDYNANRPHSAHGELTPHQFALQWTATHQPQAA
Blast result :
Comments
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
ISMyma8 orfAB is 67% aa similar to ISAar43.
ISMyma8 was characterized by Jessica Miller, with ISquest program.
ISMyma8 orfAB is 67% aa similar to ISAar43.
ISMyma8 was characterized by Jessica Miller, with ISquest program.
References
1] Gauthier D.T. (2014) Direct submission to ISfinder
2] Gauthier, D.T., Helenthal, A.M., Rhodes, M.W., Vogelbein, W.K., Kator, H.I. (2011) Dis. Aquat. Org. 95: 113-124
2] Gauthier, D.T., Helenthal, A.M., Rhodes, M.W., Vogelbein, W.K., Kator, H.I. (2011) Dis. Aquat. Org. 95: 113-124