ISfinder

ISMpr2

Family ISKra4
Group ISKra4

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NZ_GL890840	ND	Moorea producens	Moorea producens 3L

DNA section

IS Length : 1735 bp

Ends

IR Length : 19/24

IRL : GGGTGCGGCTCTTTTAAAAGTACGGTAGACTAGATCTCAGTAAAACTGAC
IRR : GGGTGTGGCTCTTTTGGAAAGACGTTTGGTCAGGTTTAGAGAGGTATCGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
CCTTATTCAC	AAATCTTTT	CTGGAAGTTC	9
CCTTATTCAC	AAATCTTTT	CTGGAAGTTC	9
CAGCTATTCT	AAATCATTT	GTAAACTATG	9
GAGCAATCCT	AAATCATTT	GTCAAATCAC	9
ACTGCTCTAC	ATATTATTT	AGCGGTTTTT	9

DNA sequence

GGGTGCGGCTCTTTTAAAAGTACGGTAGACTAGATCTCAGTAAAACTGACAAATTAGTCAGAGTAATAGTTTGAGTCAATACCTCCTGGTGGGTGACCAG
CTGAAAAAGTCAGCAGTAGTTCAGGAAAAGTTTGGACCTATGCAACCATCGATCTCAAATGAAGACATGTTTTTCTCACAAGCGCGAGAGCTATTTGAAG
AAATAGTGGACTGGCTGGGTTCAGATAGTGTTTGCGGTTTACAACATGGGGAATTAGAGAGTAAACTGCTTGAAAACGGATACGAACTATTAAGACGACT
GCTGCAAGGTTACTTCGACAGACGAAGTGATGACGAGATCGAAGGAGAATGCTTAGGTAACGACGAAGCCAAGAGAACCCATAAGAAGAGATTTACAAGG
AAGCTGACAACAATATTTGGCACGATCATCGCCAATCGAATCGGTTATGGTGGACGAAAAATAACTTCCCTCAACCCACTCGATGCCGAGTTAAACCTAC
CAGTAGAAAAATACTCCCACGGACTGAGAGAGCGAGTGGCTGTCGAAGTAGCTCGTTCAGGGTTTGGCGAAACAGTCGAAATCATACAGAAAACAACAGC
CGCAAAAGTTGGTAAGCGACAAGTTGAACAATTGGCTGATCAAAGTGCTTGTGACTTTGATCAATTCTATGCTCATCAACAAGCACAATCGGTTGAATTG
AAGGAGACGGGGGAAATAGTAGTTATTAGTGTGGATGGCAAGGGTGTTGTCATGCGTACAGAAGACTTACGTGCTCCCACGCAAAAAAGAGCGCTGGCAA
ATAGCAAGAAGTTAAATAAACGATTAACTAAGGGGGAAAAACGCAATTCAAAGCGAATGGCAACAGTGGCTTCAGTTTATACCATCAACCCTTTTGTTCG
TACACCTCAACAAATTGTCAGCACAGAAGAAGAACACAAAAAAATCAAACGACCCAAACCAATCGGTAAGCGTGTTTGGGCAAGTTTAGTTAAAGAACCA
TCACTTGTTATAAAAGAAGCTTTTGACGAGGCATTACACCGCGACCCCAATCAACACAAGCGTTTTTGCGCTCTGGTCGATGGTAACAAGACACAATTAT
CGCGCTTTGAAAAAATTTGCTCGCGAACACCATCTCAAGTTAACGATTGTCCTGGATATTATTCATGTGATTGAGTATCTGTGGAAGGCTGCATTTGCCT
TCTATTCGGACACTAGTCAACAAGCCGAAGTTTGGGTTAGTAAACGTTTACTGCTGATCCTTGAAGGTAAATCGAGTACAGTTGCTGGGGGTATGCGTGG
TAGCGCTACAAAGCGCCAACTTTCTGCATCACAACGTCAGCCAGTGGATAAGTGTGCCAGATATTTACTCAACAACGCTGCTTACCTCAAATACCACGAT
TACTTGAAAGCTGGTCTTCCCATTGCCACTGGGGTTATTGAGGGCGCTTGTCGTCACTTAATCAAAGACAGGATGGACATTACCGGGGCTAGATGGAGTC
TTATGGGTGCTGAGGCGGTTTTACGTCTGCGCTCGCTTTATGTTAGCGGTGATTGGCAAGAGTATTGGCCCTTTCACTTAAAGCAAGAGCATAAACGTAA
TCACTTGTCTTTGTACAAAGATGGCCTTCCTTTGATGAAACGACTAATTCAAGCTCGTTGCTCTATTACTGCTCCTCCTACTCTTCCGATACCTCTCTAA
ACCTGACCAAACGTCTTTCCAAAAGAGCCACACCC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
1104 bp	367 aa	71	1174	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
687 bp	228 aa	1014	1700	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

KKLLTRHYTATPINTSVFALWSMVTRHNYRALKKFAREHHLKLTIVLDIIHVIEYLWKAAFAFYSDTSQQAEVWVSKRLLLILEGKSSTVAGGMRGSATK
RQLSASQRQPVDKCARYLLNNAAYLKYHDYLKAGLPIATGVIEGACRHLIKDRMDITGARWSLMGAEAVLRLRSLYVSGDWQEYWPFHLKQEHKRNHLSL
YKDGLPLMKRLIQARCSITAPPTLPIPL

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1630 bp	543 aa	71	1700	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSQYLLVGDQLKKSAVVQEKFGPMQPSISNEDMFFSQARELFEEIVDWLGSDSVCGLQHGELESKLLENGYELLRRLLQGYFDRRSDDEIEGECLGNDEA
KRTHKKRFTRKLTTIFGTIIANRIGYGGRKITSLNPLDAELNLPVEKYSHGLRERVAVEVARSGFGETVEIIQKTTAAKVGKRQVEQLADQSACDFDQFY
AHQQAQSVELKETGEIVVISVDGKGVVMRTEDLRAPTQKRALANSKKLNKRLTKGEKRNSKRMATVASVYTINPFVRTPQQIVSTEEEHKKIKRPKPIGK
RVWASLVKEPSLVIKEAFDEALHRDPNQHKRFCALVDGNKTQLSRFEKKFAREHHLKLTIVLDIIHVIEYLWKAAFAFYSDTSQQAEVWVSKRLLLILEG
KSSTVAGGMRGSATKRQLSASQRQPVDKCARYLLNNAAYLKYHDYLKAGLPIATGVIEGACRHLIKDRMDITGARWSLMGAEAVLRLRSLYVSGDWQEYW
PFHLKQEHKRNHLSLYKDGLPLMKRLIQARCSITAPPTLPIPL

Blast result :

Comments

ISMpr2 is 88% aa similar to ISCasp3.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2013)
2] Jones,A.C., Monroe,E.A., Podell,S., Hess,W.R., Klages,S.,
Esquenazi,E., Niessen,S., Hoover,H., Rothmann,M., Lasken,R.S., Yates,J.R. III, Reinhardt,R., Kube,M., Burkart,M.D., Allen,E.E., Dorrestein,P.C., Gerwick,W.H. and Gerwick,L. (2011) Proc. Natl. Acad. Sci. U.S.A. 108 (21), 8815-8820.