ISMich2
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NZ_DS989857 | ND | Microcoleus chthonoplastes | Microcoleus chthonoplastes PCC 7420 |
DNA section
IS Length : 1805 bp
Ends
IR Length : 23/25
IRL : GGGAGCATCCCATTTTGGCAAATAGCCGGGAAAGGTCGATTTTTAATTCG
IRR : GGGAGCATCCCATTTTGGAAAAGAGAGAAAAGTTATTCGCGACTATAACT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TTAGATCCGG | CATTAAGA | GGAGATCCGA | 8 |
AGGTAACACA | AGAATGTG | AGGTTTATAC | 8 |
CTATAACCAA | TGGTCGCA | AGGGAAAGTT | 8 |
DNA sequence
GGGAGCATCCCATTTTGGCAAATAGCCGGGAAAGGTCGATTTTTAATTCGCGGTTATTACTCGCGAATTAAATCTCCGTGTATACTTGAACGACTCAAGG
TGACATAATATAGATGGAGATATTCACAAAGTTTACTGATGAACCAAGCCGAACGCCTGGAACTGGAAGCTTGTTTAAGACGAGCAAGTGAGATTCTGTA
CAACAATAGTGACACAGACAGTTTAGAAACCCTAGCTGACATCGAAATTACAGTCAGAAACCAAATTTTAGAATACGTTAGTCCCCAAATCGCCCTTTTT
TTATCACCCAAAAAACCCAAACCCGACGCGGGAAAACCCGAACCCTAAAAAGCTGCCTGGGAAAACTAAAGATTACCAAAAAACAAGCAGATGCTTTAGG
GATAGAAGCCTATACACGCCATAGCCCTTTGCTAGAAAAATGCTGTCTTCTTTTAAGCGCCAATGAATCTTATCAAAATGCGGAGAGTGATTTGTATTTG
TTAACGGGGCTAAAAGTTGGTCACTCCACGCATCATCGGAAAGTGAATCAAATTGAATTACCGCTTCCGGATCTCAAACAAGGATTATCAGAGATAAGCG
TTGATGGCGGAAAAATTCGCTTGAGGACGGCAGAGAAAGGAGAACCGAGTTTTTGGCAAGAATATAAAACAGCTCGCTTACAAGGAATTTATTACGGAGC
TTTCTTTCAAGATAATCTATCTTTAACGGATTGGATAAATAGTCAAACATTAACCTCACCCTTGTACTGTTTAGGGGATGGACATGATGGGATATGGAAC
ATCTTTGCCGAAATTGGGGAGAATAAGGATAGAATCGAAGTTTTAGACTGGTATCACCTGACCGAAAACCTGCATAAAATAGAAACCAAAAAATCTATCA
AAGACCAGTTAGAAGCTGACCTGTGGACGGGACAGGCATCCGAGGCGATAAAGTTGTTGAAGTCAGCTCAACCCCTAGGAGGAAACAACTTTATTAGCTA
TCTAAACAAGCACCGTCAAAGGTTAATCAATTATCAATCTTATCAACAGGAACAAATTTGTTCAATTGGCAGCGGAGCCGTCGAGTCAGCCGTCAAACAA
ATTAGCCATCGTGTTAAACTAACTGGCGCCCAGTGGCTCAAAGAAAACGTGGTTAACATTTTACAACTTCGTTGTGCTTATTTAAACGGTCAACTGGCTA
TTTAATGCCAACTTCCCTAATTATTAAGCGCATTTTTACAGAGAATTGATATAAGGACTTGAAGTACCGCCCATTTTTGATAAATTCGCGAGTTAGAATG
GCGAATATATCATCATGGAGATAGAAAAATGGGCAAAAGGGTTTCCAGAAAGTCACAACACGATAAGCTGGAAAGAATAACTCGTTTACAGACGGCAATT
GCTAGTCTTGAAACCTACCAAAGTTTCTTTGAGCGTCAAGGAAAACTCGCCCCAGCAGATGCTTGGGTAGCACGTTATCAAGTCCGACAGTCACAAAAAG
TTTACTGGTACTACAAACTACAGGCTAGTTCACCCACTTTCACAACCACAAGTGACACACACAAGTTAAGTAAATATAAGCACTTAGGTAAGGCTGGATG
TGAAGCCCATGTTGCTTCTGTGATGTCTGTCGCCAGAAGAACGATAGTGTCCGAATTACAAAAAACTATTGATTCTCTTAAAAATAGCTTACTAGATATT
TCTTTTGACTCTGAGCAAGAAGATATCTAATATTTAGATTAAGTAATTATTCGCGAGTTATAGTCGCGAATAACTTTTCTCTCTTTTCCAAAATGGGATG
CTCCC
TGACATAATATAGATGGAGATATTCACAAAGTTTACTGATGAACCAAGCCGAACGCCTGGAACTGGAAGCTTGTTTAAGACGAGCAAGTGAGATTCTGTA
CAACAATAGTGACACAGACAGTTTAGAAACCCTAGCTGACATCGAAATTACAGTCAGAAACCAAATTTTAGAATACGTTAGTCCCCAAATCGCCCTTTTT
TTATCACCCAAAAAACCCAAACCCGACGCGGGAAAACCCGAACCCTAAAAAGCTGCCTGGGAAAACTAAAGATTACCAAAAAACAAGCAGATGCTTTAGG
GATAGAAGCCTATACACGCCATAGCCCTTTGCTAGAAAAATGCTGTCTTCTTTTAAGCGCCAATGAATCTTATCAAAATGCGGAGAGTGATTTGTATTTG
TTAACGGGGCTAAAAGTTGGTCACTCCACGCATCATCGGAAAGTGAATCAAATTGAATTACCGCTTCCGGATCTCAAACAAGGATTATCAGAGATAAGCG
TTGATGGCGGAAAAATTCGCTTGAGGACGGCAGAGAAAGGAGAACCGAGTTTTTGGCAAGAATATAAAACAGCTCGCTTACAAGGAATTTATTACGGAGC
TTTCTTTCAAGATAATCTATCTTTAACGGATTGGATAAATAGTCAAACATTAACCTCACCCTTGTACTGTTTAGGGGATGGACATGATGGGATATGGAAC
ATCTTTGCCGAAATTGGGGAGAATAAGGATAGAATCGAAGTTTTAGACTGGTATCACCTGACCGAAAACCTGCATAAAATAGAAACCAAAAAATCTATCA
AAGACCAGTTAGAAGCTGACCTGTGGACGGGACAGGCATCCGAGGCGATAAAGTTGTTGAAGTCAGCTCAACCCCTAGGAGGAAACAACTTTATTAGCTA
TCTAAACAAGCACCGTCAAAGGTTAATCAATTATCAATCTTATCAACAGGAACAAATTTGTTCAATTGGCAGCGGAGCCGTCGAGTCAGCCGTCAAACAA
ATTAGCCATCGTGTTAAACTAACTGGCGCCCAGTGGCTCAAAGAAAACGTGGTTAACATTTTACAACTTCGTTGTGCTTATTTAAACGGTCAACTGGCTA
TTTAATGCCAACTTCCCTAATTATTAAGCGCATTTTTACAGAGAATTGATATAAGGACTTGAAGTACCGCCCATTTTTGATAAATTCGCGAGTTAGAATG
GCGAATATATCATCATGGAGATAGAAAAATGGGCAAAAGGGTTTCCAGAAAGTCACAACACGATAAGCTGGAAAGAATAACTCGTTTACAGACGGCAATT
GCTAGTCTTGAAACCTACCAAAGTTTCTTTGAGCGTCAAGGAAAACTCGCCCCAGCAGATGCTTGGGTAGCACGTTATCAAGTCCGACAGTCACAAAAAG
TTTACTGGTACTACAAACTACAGGCTAGTTCACCCACTTTCACAACCACAAGTGACACACACAAGTTAAGTAAATATAAGCACTTAGGTAAGGCTGGATG
TGAAGCCCATGTTGCTTCTGTGATGTCTGTCGCCAGAAGAACGATAGTGTCCGAATTACAAAAAACTATTGATTCTCTTAAAAATAGCTTACTAGATATT
TCTTTTGACTCTGAGCAAGAAGATATCTAATATTTAGATTAAGTAATTATTCGCGAGTTATAGTCGCGAATAACTTTTCTCTCTTTTCCAAAATGGGATG
CTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 4
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
210 bp | 69 aa | 139 | 348 | + | No |
Description : First part of the transposase
ORF sequence :
MNQAERLELEACLRRASEILYNNSDTDSLETLADIEITVRNQILEYVSPQIALFLSPKKPKPDAGKPEP
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
924 bp | 307 aa | 282 | 1205 | + | No |
Description : Second part of the transposase
ORF sequence :
SPNRPFFITQKTQTRRGKTRTLKSCLGKLKITKKQADALGIEAYTRHSPLLEKCCLLLSANESYQNAESDLYLLTGLKVGHSTHHRKVNQIELPLPDLKQ
GLSEISVDGGKIRLRTAEKGEPSFWQEYKTARLQGIYYGAFFQDNLSLTDWINSQTLTSPLYCLGDGHDGIWNIFAEIGENKDRIEVLDWYHLTENLHKI
ETKKSIKDQLEADLWTGQASEAIKLLKSAQPLGGNNFISYLNKHRQRLINYQSYQQEQICSIGSGAVESAVKQISHRVKLTGAQWLKENVVNILQLRCAY
LNGQLAI
GLSEISVDGGKIRLRTAEKGEPSFWQEYKTARLQGIYYGAFFQDNLSLTDWINSQTLTSPLYCLGDGHDGIWNIFAEIGENKDRIEVLDWYHLTENLHKI
ETKKSIKDQLEADLWTGQASEAIKLLKSAQPLGGNNFISYLNKHRQRLINYQSYQQEQICSIGSGAVESAVKQISHRVKLTGAQWLKENVVNILQLRCAY
LNGQLAI
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1067 bp | 355 aa | 139 | 1205 | + | Yes |
Chemistry : DDE
ORF sequence :
MNQAERLELEACLRRASEILYNNSDTDSLETLADIEITVRNQILEYVSPQIALFFITQKTQTRRGKTRTLKSCLGKLKITKKQADALGIEAYTRHSPLLE
KCCLLLSANESYQNAESDLYLLTGLKVGHSTHHRKVNQIELPLPDLKQGLSEISVDGGKIRLRTAEKGEPSFWQEYKTARLQGIYYGAFFQDNLSLTDWI
NSQTLTSPLYCLGDGHDGIWNIFAEIGENKDRIEVLDWYHLTENLHKIETKKSIKDQLEADLWTGQASEAIKLLKSAQPLGGNNFISYLNKHRQRLINYQ
SYQQEQICSIGSGAVESAVKQISHRVKLTGAQWLKENVVNILQLRCAYLNGQLAI
KCCLLLSANESYQNAESDLYLLTGLKVGHSTHHRKVNQIELPLPDLKQGLSEISVDGGKIRLRTAEKGEPSFWQEYKTARLQGIYYGAFFQDNLSLTDWI
NSQTLTSPLYCLGDGHDGIWNIFAEIGENKDRIEVLDWYHLTENLHKIETKKSIKDQLEADLWTGQASEAIKLLKSAQPLGGNNFISYLNKHRQRLINYQ
SYQQEQICSIGSGAVESAVKQISHRVKLTGAQWLKENVVNILQLRCAYLNGQLAI
Blast result :ORF 4
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
402 bp | 133 aa | 1329 | 1730 | + | No |
Annotation : Hypothetical proteinDescription :
ORF sequence :
MGKRVSRKSQHDKLERITRLQTAIASLETYQSFFERQGKLAPADAWVARYQVRQSQKVYWYYKLQASSPTFTTTSDTHKLSKYKHLGKAGCEAHVASVMS
VARRTIVSELQKTIDSLKNSLLDISFDSEQEDI
VARRTIVSELQKTIDSLKNSLLDISFDSEQEDI
Blast result :
Comments
ISMich2 is 83% aa similar to ISCysp26.
The third ORF is the putative ORFAb transposase reconstructed in silico by possible -1 frameshift.
ORF4 is a passenger gene annotated as hypothetical protein.
The third ORF is the putative ORFAb transposase reconstructed in silico by possible -1 frameshift.
ORF4 is a passenger gene annotated as hypothetical protein.
References
1] ISfinder annotation (2013)
2] Tandeau de Marsac,N., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2008) Direct submission GenBank.
2] Tandeau de Marsac,N., Ferriera,S., Johnson,J., Kravitz,S., Beeson,K., Sutton,G., Rogers,Y.-H., Friedman,R., Frazier,M. and Venter,J.C. (2008) Direct submission GenBank.