ISMpr1
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_GL890972 | ND | Moorea producens | Lyngbya majuscula 3L Moorea producens 3L |
DNA section
IS Length : 1178 bp
Ends
IR Length : 21/24
IRL : GGGTGCATCTCAGTTTTGCAAAAATACCAAAATCATGATATAATTAATGA
IRR : GGGTGCATCTCATCTTTGTAAAAACATCATTAATAAAGCATCCCATTTAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TTTAACGCGA | TTTACTTT | TTCCGTATAT | 8 |
DNA sequence
GGGTGCATCTCAGTTTTGCAAAAATACCAAAATCATGATATAATTAATGATGAACAATTTCTAATAATGACTAAAATTCATGAACTTGAGTCCGGAAGAA
AAGCAAGAGCTAAAAGAACATTTAAAAGCTGTCGCTCAACTTTTATATCGTCACACCCCCCCAGGAAATAAGCAAAGATTTGAGGATATAGAAACTACTT
TAAGAGACTATATTCAGGAAGAAGTTTCTCCAGAAATAGCAAAATTTTTTTTTCAGAAGTCAGTCAAGTAAAAATTGGCAGACCTCGCCGAATAAAAACC
ATAGTAGGAGAAGTAGAAATCACTGATAAACAGGCGGATTATTTTGAGCTAAAACCTCATAGTCAATTAAGTCCAAAACTGGAAAAAAATGCTCTGCTTA
TTTGTGCCAATGAATCTTATAAGAAAGCAGAGGAAGATTTATGGGCAATGACGGGAATCAAAATTTCACATAGTACATTACATAGATTAGTGAAAAAGCA
AGATCTAGACTTGCCAGAATCTAGATTAGGAATACAAGAAGTAAGCTTAGATGCAGGAAAAATCCGGTTACGTACAGAGGAAAAGGGTAAAGCTTGTGAA
TGGAAAGATTATAAAGCTGTGTGTTTGAATGGAACATATATAGGAGCTTTTTTTCAAGAAAATCAAGTTTTAATAGATTGGATAAATAGTCAAAAACTTC
TCAACCCGTTCTTTTGTACAGGTGATGGTCATCAAGGAATTTGGAATATATTTGAAGAACTGGGAGAACCGGCTCAAAGAAGAGAAATATTGGATTGGTA
TCATCTAAAGGAAAATCTTTATAAAGTGGGAGGTTCCTTGAAACGTTTAGAGAAGGGAGAAAAGCTTCTCTGGTCAGGAAAAGTAGCAGAGACTTTAGAT
TTATTTAGGGGATTAAAAAAGAAAGTAGCACAAAATTTTTGTAACTATATAGAAACTCATAATAAGCGAATAATCAACTATGACTACTATCAAAAAGAAA
GAATAAGTTCCCTAGGTTCAGGAGCAATTGAATCGGGAATTAAGCAAATAGGAATGCGAGTTAAACTATCAGGATCACAATGGAATATTGAAAATGTTCC
TAAGATTCTTTCTTTACGCTGTGCTTACCTAAATGGGATGCTTTATTAATGATGTTTTTACAAAGATGAGATGCACCC
AAGCAAGAGCTAAAAGAACATTTAAAAGCTGTCGCTCAACTTTTATATCGTCACACCCCCCCAGGAAATAAGCAAAGATTTGAGGATATAGAAACTACTT
TAAGAGACTATATTCAGGAAGAAGTTTCTCCAGAAATAGCAAAATTTTTTTTTCAGAAGTCAGTCAAGTAAAAATTGGCAGACCTCGCCGAATAAAAACC
ATAGTAGGAGAAGTAGAAATCACTGATAAACAGGCGGATTATTTTGAGCTAAAACCTCATAGTCAATTAAGTCCAAAACTGGAAAAAAATGCTCTGCTTA
TTTGTGCCAATGAATCTTATAAGAAAGCAGAGGAAGATTTATGGGCAATGACGGGAATCAAAATTTCACATAGTACATTACATAGATTAGTGAAAAAGCA
AGATCTAGACTTGCCAGAATCTAGATTAGGAATACAAGAAGTAAGCTTAGATGCAGGAAAAATCCGGTTACGTACAGAGGAAAAGGGTAAAGCTTGTGAA
TGGAAAGATTATAAAGCTGTGTGTTTGAATGGAACATATATAGGAGCTTTTTTTCAAGAAAATCAAGTTTTAATAGATTGGATAAATAGTCAAAAACTTC
TCAACCCGTTCTTTTGTACAGGTGATGGTCATCAAGGAATTTGGAATATATTTGAAGAACTGGGAGAACCGGCTCAAAGAAGAGAAATATTGGATTGGTA
TCATCTAAAGGAAAATCTTTATAAAGTGGGAGGTTCCTTGAAACGTTTAGAGAAGGGAGAAAAGCTTCTCTGGTCAGGAAAAGTAGCAGAGACTTTAGAT
TTATTTAGGGGATTAAAAAAGAAAGTAGCACAAAATTTTTGTAACTATATAGAAACTCATAATAAGCGAATAATCAACTATGACTACTATCAAAAAGAAA
GAATAAGTTCCCTAGGTTCAGGAGCAATTGAATCGGGAATTAAGCAAATAGGAATGCGAGTTAAACTATCAGGATCACAATGGAATATTGAAAATGTTCC
TAAGATTCTTTCTTTACGCTGTGCTTACCTAAATGGGATGCTTTATTAATGATGTTTTTACAAAGATGAGATGCACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 192 bp | 63 aa | 80 | 271 | + | No |
Description : First part of the transposase
ORF sequence :
MNLSPEEKQELKEHLKAVAQLLYRHTPPGNKQRFEDIETTLRDYIQEEVSPEIAKFFFQKSVK
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 966 bp | 321 aa | 184 | 1149 | + | No |
Description : Second part of the transposase
ORF sequence :
GYRNYFKRLYSGRSFSRNSKIFFSEVSQVKIGRPRRIKTIVGEVEITDKQADYFELKPHSQLSPKLEKNALLICANESYKKAEEDLWAMTGIKISHSTLH
RLVKKQDLDLPESRLGIQEVSLDAGKIRLRTEEKGKACEWKDYKAVCLNGTYIGAFFQENQVLIDWINSQKLLNPFFCTGDGHQGIWNIFEELGEPAQRR
EILDWYHLKENLYKVGGSLKRLEKGEKLLWSGKVAETLDLFRGLKKKVAQNFCNYIETHNKRIINYDYYQKERISSLGSGAIESGIKQIGMRVKLSGSQW
NIENVPKILSLRCAYLNGMLY
RLVKKQDLDLPESRLGIQEVSLDAGKIRLRTEEKGKACEWKDYKAVCLNGTYIGAFFQENQVLIDWINSQKLLNPFFCTGDGHQGIWNIFEELGEPAQRR
EILDWYHLKENLYKVGGSLKRLEKGEKLLWSGKVAETLDLFRGLKKKVAQNFCNYIETHNKRIINYDYYQKERISSLGSGAIESGIKQIGMRVKLSGSQW
NIENVPKILSLRCAYLNGMLY
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1070 bp | 356 aa | 80 | 1149 | + | Yes |
Chemistry : DDE
ORF sequence :
MNLSPEEKQELKEHLKAVAQLLYRHTPPGNKQRFEDIETTLRDYIQEEVSPEIAKFFFSEVSQVKIGRPRRIKTIVGEVEITDKQADYFELKPHSQLSPK
LEKNALLICANESYKKAEEDLWAMTGIKISHSTLHRLVKKQDLDLPESRLGIQEVSLDAGKIRLRTEEKGKACEWKDYKAVCLNGTYIGAFFQENQVLID
WINSQKLLNPFFCTGDGHQGIWNIFEELGEPAQRREILDWYHLKENLYKVGGSLKRLEKGEKLLWSGKVAETLDLFRGLKKKVAQNFCNYIETHNKRIIN
YDYYQKERISSLGSGAIESGIKQIGMRVKLSGSQWNIENVPKILSLRCAYLNGMLY
LEKNALLICANESYKKAEEDLWAMTGIKISHSTLHRLVKKQDLDLPESRLGIQEVSLDAGKIRLRTEEKGKACEWKDYKAVCLNGTYIGAFFQENQVLID
WINSQKLLNPFFCTGDGHQGIWNIFEELGEPAQRREILDWYHLKENLYKVGGSLKRLEKGEKLLWSGKVAETLDLFRGLKKKVAQNFCNYIETHNKRIIN
YDYYQKERISSLGSGAIESGIKQIGMRVKLSGSQWNIENVPKILSLRCAYLNGMLY
Blast result :
Comments
ISMpr1 is 79% aa similar to ISOni1.
The third ORF is the putative ORFAB transposase reconstructed ins iclico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed ins iclico by possible -1 frameshift.
References
1] ISfinder annotation (2013)
2] Jones,A.C., Monroe,E.A., Podell,S., Hess,W.R., Klages,S., Esquenazi,E., Niessen,S., Hoover,H., Rothmann,M., Lasken,R.S., Yates,J.R. III, Reinhardt,R., Kube,M., Burkart,M.D., Allen,E.E., Dorrestein,P.C., Gerwick,W.H. and Gerwick,L. (2010) Direct submission GenBank.
2] Jones,A.C., Monroe,E.A., Podell,S., Hess,W.R., Klages,S., Esquenazi,E., Niessen,S., Hoover,H., Rothmann,M., Lasken,R.S., Yates,J.R. III, Reinhardt,R., Kube,M., Burkart,M.D., Allen,E.E., Dorrestein,P.C., Gerwick,W.H. and Gerwick,L. (2010) Direct submission GenBank.