ISMic1
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_019738 | ND | Microcoleus | Microcoleus sp. PCC 7113 plasmid pMIC7113.04 sp. PCC 7113 Microcoleus |
DNA section
IS Length : 1773 bp
Ends
IR Length : 22/24
IRL : GGGAGCATCCCAATTTTGCAAGAAGGCAAGAGAACGGGAAGTTTATTCGC
IRR : GGGAGCATCCCAATTGTGCAAAAATAAAGGGACAGCCATTATTCGCGAGT
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TACTGTCCCT | ATCAGCTA | TCACTAAACT | 8 |
TGCCTGTTCC | AACTCGAAGA | 0 | |
TTAATGGGCG | ATCGGCTT | TGATGCGATT | 8 |
CACAAGCTTC | CTCTAATA | GATGAGTTCC | 8 |
ACTCTAGAGA | GTTTTTTC | TGAAAAAGGC | 8 |
GAATTAGTAA | TAATACCCC | ACTGTACCGT | 9 |
AACTTATGCT | GATGAGCT | TCGGCATTTT | 8 |
CTCTCTTAAG | GACACATGAG | 0 | |
CCTCCAATCT | ATCTTCTA | TGAGTTTCTA | 8 |
AAAGAGGGCG | ATACAGAA | CCCTTTCGCG | 8 |
DNA sequence
GGGAGCATCCCAATTTTGCAAGAAGGCAAGAGAACGGGAAGTTTATTCGCGATTTTTACTCGCGAATAAAGGCCGACTAGGCGGATGTTGCCCTAACTGC
GACAATTTAATTAGTTAGGGAGCCTCTTTATGACAATGACACCAGAAGACAAAAAACTTTTAGCCGACCATATCAAGGCGATAGCTAAAATTCTCTATAA
AAATACTCCACAAGAGAAAATTGAGACGTTAGAGGGTATTGAAACAGCGGTGCGCGACCAAGTGCTAGAACATGTCAGCCCCCAAATAACCTTTTTTTTA
TCGGAGAAAAGACGGGAACAGAATCGGGACGCATCCGAACGATAAGAAGTTGTGTGGGTCGGATAAAAATCACTCAAAAACAAGCGTCACGTTTAGGAAT
TGAGCCGTATCGACGGTTAAGTCCTCTTCTGGAAAAATGCTGTTTATTACTTGCAGCAAATGAATCATTTCAAGATGCAGAGAATGACCTGAAAGTCTTG
ACCGGGGTAGAAGTAGGTCACAGCACTCATCATCGCCAAGCCCAGAAAGTAGAGCTATCGCCACCTAATATTAAACAAAAACTGACAGAAGTTTGTCTCG
ATGGTGGGAAAGTGCGTTTACGCTCACAGGAAAAAGGTAAACCCGCCTACTGGAAGGAGTATAAAACCGGACGACTACAAGGAATATATTACGGAGCCTT
CTTTCAAGATAATTTTTCTCTAATTAATTGGGTGAACAGCCAAAACCTGGCTCGAACCATTTATTGCTTGGGTGATGGACACGATGGGGTGTGGAACCTA
TTTGCACAAATAGCCGACGACCAGACCCGACAAGAAATTCTTGACTGGTATCATTTGAAAGAGAACCTCTATAAAATTCAGGCATCGAAAAAGTTTTTAG
AACAAATCGAAGCAGATTTGTGGCAAGGAGTGGTCGAAGAAGCGATTAGTAAACTCCGGAAAACTAACTATGTCGGGGCTACTAACTTTATAAGTTATCT
ACGTAAACATAGGCATCGCTTAGTCAATTATATGTACTTTCAAGCCGAACAATTAAGTTCGATTGCTTCGGGAGCTGTAGAGTCTGCGGTCAAACAGATA
GACAAGCGGCTACAAATAGTCGGCGCTCAATGGAAGTCCCAAAATTTACCGCAAATGCTGCAACTGCGGTGCGCTTATCTGAATCGACAGCTTGCTCCAA
GCGCTTAAAAAACCCAAAAACAGTATGAAAAGAAGCACGCTGCTTTTTTTCGCGAGTTACAATCGCGAATAATTAGGATTTATCTTTATGCCTCGAAAGA
CTTCCAAGGATATTGAGCGAGATAAACTCGCTCGATTTGAGCGTATTAAAAAGGCGATAGCAGCGTTAGAAAAACAACAAAGACAAATATTAAAACAGGG
TGAGCTGGCTCCATCCGGGGCTTGGGTAGCGCGTTATCAAGTTCGGCAAAATAATAAGAAATATTGGTACTACAAATTACAAGTGCCGACTCCATACTTC
CCATGCGCGACCTCCGAAGAGTTGAGTAAATACAAGCATCTGGGGAAAGCTGGTACTACTGACCATATAGATGCTGTCATGTCTGTTTTTAGGCGTTCTG
TTATCGATGAGATACAGAAGCTCATCCGTTCCCTAGATGATTGCTTGCTGGATATTACTTCTGGAGCCGAGCAGGAGGAAGAAAAACCGCAGGATTGAAT
CTCCCGAAGTATTCGCGATTATTACTCGCGAATAATGGCTGTCCCTTTATTTTTGCACAATTGGGATGCTCCC
GACAATTTAATTAGTTAGGGAGCCTCTTTATGACAATGACACCAGAAGACAAAAAACTTTTAGCCGACCATATCAAGGCGATAGCTAAAATTCTCTATAA
AAATACTCCACAAGAGAAAATTGAGACGTTAGAGGGTATTGAAACAGCGGTGCGCGACCAAGTGCTAGAACATGTCAGCCCCCAAATAACCTTTTTTTTA
TCGGAGAAAAGACGGGAACAGAATCGGGACGCATCCGAACGATAAGAAGTTGTGTGGGTCGGATAAAAATCACTCAAAAACAAGCGTCACGTTTAGGAAT
TGAGCCGTATCGACGGTTAAGTCCTCTTCTGGAAAAATGCTGTTTATTACTTGCAGCAAATGAATCATTTCAAGATGCAGAGAATGACCTGAAAGTCTTG
ACCGGGGTAGAAGTAGGTCACAGCACTCATCATCGCCAAGCCCAGAAAGTAGAGCTATCGCCACCTAATATTAAACAAAAACTGACAGAAGTTTGTCTCG
ATGGTGGGAAAGTGCGTTTACGCTCACAGGAAAAAGGTAAACCCGCCTACTGGAAGGAGTATAAAACCGGACGACTACAAGGAATATATTACGGAGCCTT
CTTTCAAGATAATTTTTCTCTAATTAATTGGGTGAACAGCCAAAACCTGGCTCGAACCATTTATTGCTTGGGTGATGGACACGATGGGGTGTGGAACCTA
TTTGCACAAATAGCCGACGACCAGACCCGACAAGAAATTCTTGACTGGTATCATTTGAAAGAGAACCTCTATAAAATTCAGGCATCGAAAAAGTTTTTAG
AACAAATCGAAGCAGATTTGTGGCAAGGAGTGGTCGAAGAAGCGATTAGTAAACTCCGGAAAACTAACTATGTCGGGGCTACTAACTTTATAAGTTATCT
ACGTAAACATAGGCATCGCTTAGTCAATTATATGTACTTTCAAGCCGAACAATTAAGTTCGATTGCTTCGGGAGCTGTAGAGTCTGCGGTCAAACAGATA
GACAAGCGGCTACAAATAGTCGGCGCTCAATGGAAGTCCCAAAATTTACCGCAAATGCTGCAACTGCGGTGCGCTTATCTGAATCGACAGCTTGCTCCAA
GCGCTTAAAAAACCCAAAAACAGTATGAAAAGAAGCACGCTGCTTTTTTTCGCGAGTTACAATCGCGAATAATTAGGATTTATCTTTATGCCTCGAAAGA
CTTCCAAGGATATTGAGCGAGATAAACTCGCTCGATTTGAGCGTATTAAAAAGGCGATAGCAGCGTTAGAAAAACAACAAAGACAAATATTAAAACAGGG
TGAGCTGGCTCCATCCGGGGCTTGGGTAGCGCGTTATCAAGTTCGGCAAAATAATAAGAAATATTGGTACTACAAATTACAAGTGCCGACTCCATACTTC
CCATGCGCGACCTCCGAAGAGTTGAGTAAATACAAGCATCTGGGGAAAGCTGGTACTACTGACCATATAGATGCTGTCATGTCTGTTTTTAGGCGTTCTG
TTATCGATGAGATACAGAAGCTCATCCGTTCCCTAGATGATTGCTTGCTGGATATTACTTCTGGAGCCGAGCAGGAGGAAGAAAAACCGCAGGATTGAAT
CTCCCGAAGTATTCGCGATTATTACTCGCGAATAATGGCTGTCCCTTTATTTTTGCACAATTGGGATGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 4
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
216 bp | 71 aa | 130 | 345 | + | No |
Description : First part of the transposase
ORF sequence :
MTMTPEDKKLLADHIKAIAKILYKNTPQEKIETLEGIETAVRDQVLEHVSPQITFFLSEKRREQNRDASER
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
966 bp | 321 aa | 243 | 1208 | + | No |
Description : Second part of the transposase
ORF sequence :
NSGARPSARTCQPPNNLFFIGEKTGTESGRIRTIRSCVGRIKITQKQASRLGIEPYRRLSPLLEKCCLLLAANESFQDAENDLKVLTGVEVGHSTHHRQA
QKVELSPPNIKQKLTEVCLDGGKVRLRSQEKGKPAYWKEYKTGRLQGIYYGAFFQDNFSLINWVNSQNLARTIYCLGDGHDGVWNLFAQIADDQTRQEIL
DWYHLKENLYKIQASKKFLEQIEADLWQGVVEEAISKLRKTNYVGATNFISYLRKHRHRLVNYMYFQAEQLSSIASGAVESAVKQIDKRLQIVGAQWKSQ
NLPQMLQLRCAYLNRQLAPSA
QKVELSPPNIKQKLTEVCLDGGKVRLRSQEKGKPAYWKEYKTGRLQGIYYGAFFQDNFSLINWVNSQNLARTIYCLGDGHDGVWNLFAQIADDQTRQEIL
DWYHLKENLYKIQASKKFLEQIEADLWQGVVEEAISKLRKTNYVGATNFISYLRKHRHRLVNYMYFQAEQLSSIASGAVESAVKQIDKRLQIVGAQWKSQ
NLPQMLQLRCAYLNRQLAPSA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1079 bp | 358 aa | 130 | 1208 | + | Yes |
Chemistry : DDE
ORF sequence :
MTMTPEDKKLLADHIKAIAKILYKNTPQEKIETLEGIETAVRDQVLEHVSPQITFFIGEKTGTESGRIRTIRSCVGRIKITQKQASRLGIEPYRRLSPLL
EKCCLLLAANESFQDAENDLKVLTGVEVGHSTHHRQAQKVELSPPNIKQKLTEVCLDGGKVRLRSQEKGKPAYWKEYKTGRLQGIYYGAFFQDNFSLINW
VNSQNLARTIYCLGDGHDGVWNLFAQIADDQTRQEILDWYHLKENLYKIQASKKFLEQIEADLWQGVVEEAISKLRKTNYVGATNFISYLRKHRHRLVNY
MYFQAEQLSSIASGAVESAVKQIDKRLQIVGAQWKSQNLPQMLQLRCAYLNRQLAPSA
EKCCLLLAANESFQDAENDLKVLTGVEVGHSTHHRQAQKVELSPPNIKQKLTEVCLDGGKVRLRSQEKGKPAYWKEYKTGRLQGIYYGAFFQDNFSLINW
VNSQNLARTIYCLGDGHDGVWNLFAQIADDQTRQEILDWYHLKENLYKIQASKKFLEQIEADLWQGVVEEAISKLRKTNYVGATNFISYLRKHRHRLVNY
MYFQAEQLSSIASGAVESAVKQIDKRLQIVGAQWKSQNLPQMLQLRCAYLNRQLAPSA
Blast result :ORF 4
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
411 bp | 136 aa | 1288 | 1698 | + | No |
Annotation : Hypothetical proteinDescription :
ORF sequence :
MPRKTSKDIERDKLARFERIKKAIAALEKQQRQILKQGELAPSGAWVARYQVRQNNKKYWYYKLQVPTPYFPCATSEELSKYKHLGKAGTTDHIDAVMSV
FRRSVIDEIQKLIRSLDDCLLDITSGAEQEEEKPQD
FRRSVIDEIQKLIRSLDDCLLDITSGAEQEEEKPQD
Blast result :
Comments
ISMic1 is 67% aa (ORFAB : the transposase) similar to ISOni1.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ORF4 is a passenger gene annotated as hypothetical protein.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ORF4 is a passenger gene annotated as hypothetical protein.
References
1] ISfinder annotation (2013)
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T. and Kerfeld,C.A. (2012) Direct submission GenBank.
2] Gugger,M., Coursin,T., Rippka,R., Tandeau De Marsac,N., Huntemann,M., Wei,C.-L., Han,J., Detter,J.C., Han,C., Tapia,R., Chen,A., Kyrpides,N., Mavromatis,K., Markowitz,V., Szeto,E., Ivanova,N., Pagani,I., Pati,A., Goodwin,L., Nordberg,H.P., Cantor,M.N., Hua,S.X., Woyke,T. and Kerfeld,C.A. (2012) Direct submission GenBank.