ISMal1

  • Family ISKra4
  • Group ISAzba1
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NZ_AHAM01000308 ND Mesorhizobium alhagi
Mesorhizobium alhagi CCNWXJ12-2
DNA section
IS Length : 2308 bp

Ends


IR Length : 19/21

IRL : GAGAGCAGGAAAAAGTGTGGGATGTTTCCGGTAGTCCGAGCGAGAGAGCG
IRR : GAGAGCAGGAAAATCTGTGGGGGCATTCAGGCGGCCGCAGCTTGAGTTGA

Insertion site


Left flankDirect repeatRight flankDR Length
TTCGATGAAGGATCTATTCA0

DNA sequence

GAGAGCAGGAAAAAGTGTGGGATGTTTCCGGTAGTCCGAGCGAGAGAGCGGCGATAGACTTGTCGCGATAGCACTTTTCCGCCGGACTGAATTAGAACAT
GCTTGACGACGAGATCGACTCATCGGGTGGAGCCAACGGTTCGATCCTGCAACTGAAGGTTCGCCTGCTCGATGTGAGCCCGATGGTCTGGAGGCGCGTC
GTGGTGCCGGCAGCCATGACGTTGAAGGACCTGCATGGCGTCTTTCAGGTGGTGCTGGGGTGGCACAGCCTTCACCTTTATCGGTTTCGCATCCATGCTG
TGCACTATGGTTCCTGGGGGCTGGGAGCGGAAAATCCGGACCGAACGCTTTGCAGCTTCAATTTCCGCCGCAGTGACAAGTTCGTCTATGAATACGACAT
GACGGACTACTGGAAGCACGAGGTCCGGATCGATGCCTGGCTTGAGCCGCAGCCCCGCAATGGCTACCCGGCCTGCATCGGCGGCAAGGGTGGTTGCCCG
CCCGAGGAATGCGGCGGTCCGGCCGGCTACGACGAATTCCGCATCGAAGCACTCGGCCTCGATGCCCACGAAGATCTCGACACCATGGTCGAGATTCTGG
ATCGAATCGTGCTGCAGAACCAGCCACATCTGCTGGATGACGACGAAATCCGCGGTCGGCTCGAGCGCGCTGTGGAGCGAAACCGAGCTCGGTTTGACCG
GCGCGCAGTGAACACCCGGTTGCGCGCCGGCGAGCACCATGTTCTCAAGCATCAGCAGCTTTGCTGAGCCGGCGGGTTCGCCAGACCAAAACCGGGAGAG
CGCGGCCACGGGTTTTCCTCTCCCTGTCTTGTGACAAACTCATGCAGGAGGAGGGAAGCCATGCGTGTCGAGGTGCGATTGCGGGTTGTTGGCGACGCCG
GCGAAATCATCTGCGATGACGCCAATGTGCTGGTGATCGATAAGCGCCACGATCGGCTGGAGGCAATCGGCCTTTCGCTCGATGAAGCCAAGGAGTTTCT
GGCCGGCGTTCAGCAGCACCTCGTCGCGGCCCAGGCTGCCGCCTTCGCCGCCGGTCGTCGATGCTGCACACATTGTGGTCGCTCCCTTCGGCGCAAGGGC
CGCGCCACGCTGACGTTTCGCACCCCGTTCGGCAATGTGCCAATCGACAGTCCGCGCCTTCATCGGTGTCGCTGCGCGGCTTCGGCAGCCGAAGAAGGAC
CGCAGACCTTCAGCCCATTGACCTCGCTGTTGACCAGCCACATCGCGCCCGAGCTGCTCTATCTTGAAAGCAAATGGGCCTCGCTCGTCTCCTATGGCAT
GACCGTCGACCTGCTCAGGGACGTGCTGCCGATCGACGCCAGGCTCAACCCGAAAACCGTGCGCAATCATCTGCACCGTATGGCGGAGCGCCTCGAATCC
GTACTTGGCGATGAGCGGCCGAACTTCATCGACAGCTGTCCACGCGACTGGAAGGCGCTGCCGCAGCCGGAAGGCGAGATCGTCGTCGGCATCGATGGCG
GCTATGTGCGCGACTGGACCGACAGGAAAAGTCATTTCGGGATTCTGGTCGGCAAGTCATTGCCGCGGGATCGCAACGATCGCTATCTCGGCCTGGTCCA
GAGTTTTGATACCAAGCCGAAACGCCGACTGTGGGAACTCCTGTGTTCGCAAGGCCTGCAGCTGAACCAAGCCGTCACCTTCCTGACCGACGGCGGCGAT
GACGTTCGCAACCTGGCGGCCGAGATGGCGCCCTACGCGGAGCATCATCTGGATTGGTTCCATATCACCATGCGCCTGACGGTGCTTGGCCAGTATGCCA
AAGGTCTCATCCATCACGACCAGGAAGCCGGCGAGCGGATTGCCCGCAATCTGCTGCGGATCAAGCGGTACCTCTGGCATGGCAATTCCCGCGCCGCCCT
GTTCTGGAGCCAGGACCTAGCGGATGACCTCTATGGGCTCGAAGAAGACTGCACCTATCCCAAGCTGAAGGCGTTCTCCCAGGCGCTCGCGGAATTCGTC
ACCTATATCGAGCGCAACGCCCACGCGATCCCGAATTACGGAGAACGCTATCGCAACGACGAGCGCATCGCTACGTCGTTCGTGGAGTCCGCCGTCAATG
TCGTCATCGCCAAGCGCTTTACCAAGAAACAACAGATGCAGTGGTCGCGATGCGGCGCCCATATGCTCCTGCAGATCCGCACGCGAACCCTGGATGGCAC
GCTCCGCGGCCAATTCGAAACGTGGTATCCCGGGCTCGCGGCAAATGAGAACCAGACATCAACTCAAGCTGCGGCCGCCTGAATGCCCCCACAGATTTTC
CTGCTCTC
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
669 bp222 aa99767+No
ORF function : Passenger Gene
Annotation : plasmid pRIA4b ORF-3 family proteinDescription :

ORF sequence :

MLDDEIDSSGGANGSILQLKVRLLDVSPMVWRRVVVPAAMTLKDLHGVFQVVLGWHSLHLYRFRIHAVHYGSWGLGAENPDRTLCSFNFRRSDKFVYEYD
MTDYWKHEVRIDAWLEPQPRNGYPACIGGKGGCPPEECGGPAGYDEFRIEALGLDAHEDLDTMVEILDRIVLQNQPHLLDDDEIRGRLERAVERNRARFD
RRAVNTRLRAGEHHVLKHQQLC

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1422 bp473 aa8612282+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRVEVRLRVVGDAGEIICDDANVLVIDKRHDRLEAIGLSLDEAKEFLAGVQQHLVAAQAAAFAAGRRCCTHCGRSLRRKGRATLTFRTPFGNVPIDSPRL
HRCRCAASAAEEGPQTFSPLTSLLTSHIAPELLYLESKWASLVSYGMTVDLLRDVLPIDARLNPKTVRNHLHRMAERLESVLGDERPNFIDSCPRDWKAL
PQPEGEIVVGIDGGYVRDWTDRKSHFGILVGKSLPRDRNDRYLGLVQSFDTKPKRRLWELLCSQGLQLNQAVTFLTDGGDDVRNLAAEMAPYAEHHLDWF
HITMRLTVLGQYAKGLIHHDQEAGERIARNLLRIKRYLWHGNSRAALFWSQDLADDLYGLEEDCTYPKLKAFSQALAEFVTYIERNAHAIPNYGERYRND
ERIATSFVESAVNVVIAKRFTKKQQMQWSRCGAHMLLQIRTRTLDGTLRGQFETWYPGLAANENQTSTQAAAA

 

Blast result :
Comments
ISMal1 is 69% aa (ORFB : the transposase) similar to ISAzs26.
ORFA is a passenger gene annotated as plasmid pRiA4b ORF-3 family protein.
References
1] ISfinder annotation (2013)
2] Zhou,M., Chen,W., Chen,H. and Wei,G. (2011) Direct submission GenBank.