ISMiva1
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NZ_AFJC01000007.1 | ND | Microcoleus vaginatus | Microcoleus vaginatus FGP-2 |
DNA section
IS Length : 1165 bp
Ends
IR Length : 23/23
IRL : GGGTGCATCTCACTATTGCAAATCTCTGAAAAAAGGGCGAAAATAAAAAA
IRR : GGGTGCATCTCAGTTTGGCAAATACATCAAGTGGAAAGTTGGCCATTGAG
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GGATTTGATG | TAGTTAACCT | 0 |
DNA sequence
GGGTGCATCTCACTATTGCAAATCTCTGAAAAAAGGGCGAAAATAAAAAAGCTAGTAAAACTGTGAAAAAAAATGACACCCGAAGACCAAAAGCGCCTAA
AAGCACACTTAAAAGAGGTAGCATCCATTCTTTACAGAAATACAGATTCAACGGAACTGTCCAATTTTGAAAGTATCGAAAAATCTCTGCGTCAAAAAAT
ATTATCAGAAGTGGGACCAGAATTAGGTAGCTTTTTTTTTCAACAGCATCAGGAATTAAAACAGGAAGAACGCGAACAGTAAAATCTGTAGTTGGGTCGC
TCAGGATTACAAATAATCAGGGGAAATATTTCGGGTTAAAACCTTATAGTCAACTCAGTCCCCTGATGGAAAAGTGTGCTCTGATAATCAGAAATAATGA
GTATTATCAAAAAGGAGAAAAAAATTTAGAAACATTCATAGGAATCCCAGTGTCTCATAGTACATTACACAGATTAGTAAAGAGCCAAAAATTTGAATTG
CCTACATCTAAGCAAGGAGTCCAAGAAATTTTCTTAGACGGAGGCAAAGTCAGGCTACGCAACGAAAACAAGGGCGAGCCATGTTATTGGAAAGATTATA
AAGCCCTGTGTTTAGATAATGTTTATTGCGGAGCATTCTTTCAAAATGATCAAGATTTAATTGATTGGACTAATAGCCAAAAATTACTACACCCTATGTA
TTGGGTAGGAGATGGTCATCCCGGAATTTGGAACTTATTTCAAGAAATTGGAGTGACTGAACAAAGGCAACAAATCTTAGATTGGTATCATCTGAAAGAA
AATTTCTATAAAGCAGGGGGCTCACTGAAACGCTCAAAGCAAGCAGAAAGTCTGTTGTGGTCAGGTCAAGTTAATCAAGTGATAAATTTATTTAAAGACT
TGAAAAGAAAAGCATTTAAAACATTTTGCACTTATTTAGAAACCCATCGCAACCGAATAGTTAACTACCAATACTATCAAGAAGAGTCCCTGAGTTCTAT
CGGTTCTGGAACAGTTGAATCAACGATTAAACGCATTGGGATCAGGGTGAAATTATCGGGAGCGCAGTGGAATATCGAGAGTGTTGCCTCTATCCTTTCT
CTGCGCTGTGCTTATCTCAATGGCCAACTTTCCACTTGATGTATTTGCCAAACTGAGATGCACCC
AAGCACACTTAAAAGAGGTAGCATCCATTCTTTACAGAAATACAGATTCAACGGAACTGTCCAATTTTGAAAGTATCGAAAAATCTCTGCGTCAAAAAAT
ATTATCAGAAGTGGGACCAGAATTAGGTAGCTTTTTTTTTCAACAGCATCAGGAATTAAAACAGGAAGAACGCGAACAGTAAAATCTGTAGTTGGGTCGC
TCAGGATTACAAATAATCAGGGGAAATATTTCGGGTTAAAACCTTATAGTCAACTCAGTCCCCTGATGGAAAAGTGTGCTCTGATAATCAGAAATAATGA
GTATTATCAAAAAGGAGAAAAAAATTTAGAAACATTCATAGGAATCCCAGTGTCTCATAGTACATTACACAGATTAGTAAAGAGCCAAAAATTTGAATTG
CCTACATCTAAGCAAGGAGTCCAAGAAATTTTCTTAGACGGAGGCAAAGTCAGGCTACGCAACGAAAACAAGGGCGAGCCATGTTATTGGAAAGATTATA
AAGCCCTGTGTTTAGATAATGTTTATTGCGGAGCATTCTTTCAAAATGATCAAGATTTAATTGATTGGACTAATAGCCAAAAATTACTACACCCTATGTA
TTGGGTAGGAGATGGTCATCCCGGAATTTGGAACTTATTTCAAGAAATTGGAGTGACTGAACAAAGGCAACAAATCTTAGATTGGTATCATCTGAAAGAA
AATTTCTATAAAGCAGGGGGCTCACTGAAACGCTCAAAGCAAGCAGAAAGTCTGTTGTGGTCAGGTCAAGTTAATCAAGTGATAAATTTATTTAAAGACT
TGAAAAGAAAAGCATTTAAAACATTTTGCACTTATTTAGAAACCCATCGCAACCGAATAGTTAACTACCAATACTATCAAGAAGAGTCCCTGAGTTCTAT
CGGTTCTGGAACAGTTGAATCAACGATTAAACGCATTGGGATCAGGGTGAAATTATCGGGAGCGCAGTGGAATATCGAGAGTGTTGCCTCTATCCTTTCT
CTGCGCTGTGCTTATCTCAATGGCCAACTTTCCACTTGATGTATTTGCCAAACTGAGATGCACCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 210 bp | 69 aa | 73 | 282 | + | No |
Description : First part of the transposase
ORF sequence :
MTPEDQKRLKAHLKEVASILYRNTDSTELSNFESIEKSLRQKILSEVGPELGSFFFQQHQELKQEEREQ
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 908 bp | 302 aa | 232 | 1139 | + | No |
Description : Second part of the transposase
ORF sequence :
LFFSTASGIKTGRTRTVKSVVGSLRITNNQGKYFGLKPYSQLSPLMEKCALIIRNNEYYQKGEKNLETFIGIPVSHSTLHRLVKSQKFELPTSKQGVQEI
FLDGGKVRLRNENKGEPCYWKDYKALCLDNVYCGAFFQNDQDLIDWTNSQKLLHPMYWVGDGHPGIWNLFQEIGVTEQRQQILDWYHLKENFYKAGGSLK
RSKQAESLLWSGQVNQVINLFKDLKRKAFKTFCTYLETHRNRIVNYQYYQEESLSSIGSGTVESTIKRIGIRVKLSGAQWNIESVASILSLRCAYLNGQL
ST
FLDGGKVRLRNENKGEPCYWKDYKALCLDNVYCGAFFQNDQDLIDWTNSQKLLHPMYWVGDGHPGIWNLFQEIGVTEQRQQILDWYHLKENFYKAGGSLK
RSKQAESLLWSGQVNQVINLFKDLKRKAFKTFCTYLETHRNRIVNYQYYQEESLSSIGSGTVESTIKRIGIRVKLSGAQWNIESVASILSLRCAYLNGQL
ST
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1067 bp | 355 aa | 73 | 1139 | + | Yes |
Chemistry : DDE
ORF sequence :
MTPEDQKRLKAHLKEVASILYRNTDSTELSNFESIEKSLRQKILSEVGPELGSFFFSTASGIKTGRTRTVKSVVGSLRITNNQGKYFGLKPYSQLSPLME
KCALIIRNNEYYQKGEKNLETFIGIPVSHSTLHRLVKSQKFELPTSKQGVQEIFLDGGKVRLRNENKGEPCYWKDYKALCLDNVYCGAFFQNDQDLIDWT
NSQKLLHPMYWVGDGHPGIWNLFQEIGVTEQRQQILDWYHLKENFYKAGGSLKRSKQAESLLWSGQVNQVINLFKDLKRKAFKTFCTYLETHRNRIVNYQ
YYQEESLSSIGSGTVESTIKRIGIRVKLSGAQWNIESVASILSLRCAYLNGQLST
KCALIIRNNEYYQKGEKNLETFIGIPVSHSTLHRLVKSQKFELPTSKQGVQEIFLDGGKVRLRNENKGEPCYWKDYKALCLDNVYCGAFFQNDQDLIDWT
NSQKLLHPMYWVGDGHPGIWNLFQEIGVTEQRQQILDWYHLKENFYKAGGSLKRSKQAESLLWSGQVNQVINLFKDLKRKAFKTFCTYLETHRNRIVNYQ
YYQEESLSSIGSGTVESTIKRIGIRVKLSGAQWNIESVASILSLRCAYLNGQLST
Blast result :
Comments
ISMiva1 is 71% aa similar to ISNpu14.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Pagani,I., Land,M.L., Hauser,L., Garcia-Pichel,F., Kuske,C.R. and Woyke,T.J. (2011) Direct submission GenBank.
2] Lucas,S., Han,J., Lapidus,A., Cheng,J.-F., Goodwin,L., Pitluck,S., Pagani,I., Land,M.L., Hauser,L., Garcia-Pichel,F., Kuske,C.R. and Woyke,T.J. (2011) Direct submission GenBank.