ISMae18
- Family ISKra4
- Group ISMich2
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010296 | ND | Microcystis aeruginosa | Microcystis aeruginosa PCC 7806 Microcystis aeruginosa NIES-843 |
DNA section
IS Length : 1179 bp
Ends
IR Length : 32/38
IRL : GGGAGCATCCCAATTTTGCCATAAGTATAAATGCTTAGAGACTAAAAACG
IRR : GGGAGCATCCCAGTTTTGCAATGAGTAAAAATACTCAGGCAATATCGCCG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
TGTTAAGGAT | ATTTCCGGT | TGACCTGTCC | 9 |
CAAAAAGTCT | CTCAAAACA | CCAAACAAAAC | 9 |
ACAGGGAATT | CTAGCATCTGC | 0 | |
TTACTCGCCA | TTGGTGCTT | TTGGGTGCGGGT | 9 |
AAAACTGATA | GAATAGAACTA | 0 | |
ATATGTTCTA | TATTCTCGC | CAATATTCTA | 9 |
DNA sequence
GGGAGCATCCCAATTTTGCCATAAGTATAAATGCTTAGAGACTAAAAACGTTAGCTAAGGCAAAATAAAGAGAAACAATTAAACGGTAGAATCATGAATA
CAGACCAAAAAGAACAACTAGATCAACATTTAAAAGCCATTGCTCAAATTCTAGTCGATAATACCCCAGAAGAACAACTACGTAGCTTTGAGGGCATTGA
AACCGCCCTGCGAGACCATTGGCTGACTACATTGGGTCCTGCCATAGGCAATTTTTTTTTGAATCAGCAACAGGAACCCAAGCAGGGCGAACCAAAAGCG
TAAGCAGTATCATAGGGAAAGTCAAGATAAGCGAGAAACAAGCCGATAAACTAGGATTAAGCAAGAATAATCGATTAAGTCCCATGCTGGAGAAATGTTG
CTTAGGAGCAGTGGCTAAAGTCTCTTTTGAAGATGCGGAAAAAGCTATCAAAATGGCGACAGGAATGGCAGTTTCAGGCAGTAGTCAACAGAGGCTTGTA
CAAAGATATAAATTTGAGGAAGCAGAAGCAAAGAGTCCGGTAGAAGCATTGAGTGTAGAGGTCGGAAAAGTCAGAATCAGAACGCCCAAAGGACAACCGA
GTCAAGGTCGAGATTACAAAGCAGTAAGTCTGCATGGTCAAGAATGTGCGGGATTTTTTCAGCAAAATGAAGAATTACTGGAATGGGTGAACCGTCAGCC
CTTAACAGAGGTAGTCACCTGTTTAGGAGATGGTCATGATGGGGTGTGGAATCTGATGGAGAAAATCGGTGTTAAAAGGAGAGAAATATTGGATTGGTAT
CATTTAGTAGAGAATATGAAGAAAATAGGCGGGTCAAACAAGCGTCTGAATAGAATCAAAGAGAATTTATGGAAAGGAGAGGTGAAAAGAGTTTTAGAGG
AATTAGAGGGATGCAAAAAGAAACAGGCTATAAATTTCACCAAATATGTCGATAAACATCGAGAAAGAATACCCAATTACGAACTCTATCAATCACAAGG
GATTTGCATCGGTTCTGGAAGTGTAGAGTCAAAGATTAAGCAAATAGGTGCAAGAATGAAAATTGTGGGAGCACAATGGAAAGCAGAGAATGTTCCTCAG
TATTTGAAGCTACGTTGTGCTTATCTCAACGGCGATATTGCCTGAGTATTTTTACTCATTGCAAAACTGGGATGCTCCC
CAGACCAAAAAGAACAACTAGATCAACATTTAAAAGCCATTGCTCAAATTCTAGTCGATAATACCCCAGAAGAACAACTACGTAGCTTTGAGGGCATTGA
AACCGCCCTGCGAGACCATTGGCTGACTACATTGGGTCCTGCCATAGGCAATTTTTTTTTGAATCAGCAACAGGAACCCAAGCAGGGCGAACCAAAAGCG
TAAGCAGTATCATAGGGAAAGTCAAGATAAGCGAGAAACAAGCCGATAAACTAGGATTAAGCAAGAATAATCGATTAAGTCCCATGCTGGAGAAATGTTG
CTTAGGAGCAGTGGCTAAAGTCTCTTTTGAAGATGCGGAAAAAGCTATCAAAATGGCGACAGGAATGGCAGTTTCAGGCAGTAGTCAACAGAGGCTTGTA
CAAAGATATAAATTTGAGGAAGCAGAAGCAAAGAGTCCGGTAGAAGCATTGAGTGTAGAGGTCGGAAAAGTCAGAATCAGAACGCCCAAAGGACAACCGA
GTCAAGGTCGAGATTACAAAGCAGTAAGTCTGCATGGTCAAGAATGTGCGGGATTTTTTCAGCAAAATGAAGAATTACTGGAATGGGTGAACCGTCAGCC
CTTAACAGAGGTAGTCACCTGTTTAGGAGATGGTCATGATGGGGTGTGGAATCTGATGGAGAAAATCGGTGTTAAAAGGAGAGAAATATTGGATTGGTAT
CATTTAGTAGAGAATATGAAGAAAATAGGCGGGTCAAACAAGCGTCTGAATAGAATCAAAGAGAATTTATGGAAAGGAGAGGTGAAAAGAGTTTTAGAGG
AATTAGAGGGATGCAAAAAGAAACAGGCTATAAATTTCACCAAATATGTCGATAAACATCGAGAAAGAATACCCAATTACGAACTCTATCAATCACAAGG
GATTTGCATCGGTTCTGGAAGTGTAGAGTCAAAGATTAAGCAAATAGGTGCAAGAATGAAAATTGTGGGAGCACAATGGAAAGCAGAGAATGTTCCTCAG
TATTTGAAGCTACGTTGTGCTTATCTCAACGGCGATATTGCCTGAGTATTTTTACTCATTGCAAAACTGGGATGCTCCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
210 bp | 69 aa | 94 | 303 | + | No |
Description : First part of the transposase
ORF sequence :
MNTDQKEQLDQHLKAIAQILVDNTPEEQLRSFEGIETALRDHWLTTLGPAIGNFFLNQQQEPKQGEPKA
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
945 bp | 314 aa | 201 | 1145 | + | No |
Description : Second part of the transposase
ORF sequence :
NRPARPLADYIGSCHRQFFFESATGTQAGRTKSVSSIIGKVKISEKQADKLGLSKNNRLSPMLEKCCLGAVAKVSFEDAEKAIKMATGMAVSGSSQQRLV
QRYKFEEAEAKSPVEALSVEVGKVRIRTPKGQPSQGRDYKAVSLHGQECAGFFQQNEELLEWVNRQPLTEVVTCLGDGHDGVWNLMEKIGVKRREILDWY
HLVENMKKIGGSNKRLNRIKENLWKGEVKRVLEELEGCKKKQAINFTKYVDKHRERIPNYELYQSQGICIGSGSVESKIKQIGARMKIVGAQWKAENVPQ
YLKLRCAYLNGDIA
QRYKFEEAEAKSPVEALSVEVGKVRIRTPKGQPSQGRDYKAVSLHGQECAGFFQQNEELLEWVNRQPLTEVVTCLGDGHDGVWNLMEKIGVKRREILDWY
HLVENMKKIGGSNKRLNRIKENLWKGEVKRVLEELEGCKKKQAINFTKYVDKHRERIPNYELYQSQGICIGSGSVESKIKQIGARMKIVGAQWKAENVPQ
YLKLRCAYLNGDIA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1052 bp | 350 aa | 94 | 1145 | + | Yes |
Chemistry : DDE
ORF sequence :
MNTDQKEQLDQHLKAIAQILVDNTPEEQLRSFEGIETALRDHWLTTLGPAIGNFFFESATGTQAGRTKSVSSIIGKVKISEKQADKLGLSKNNRLSPMLE
KCCLGAVAKVSFEDAEKAIKMATGMAVSGSSQQRLVQRYKFEEAEAKSPVEALSVEVGKVRIRTPKGQPSQGRDYKAVSLHGQECAGFFQQNEELLEWVN
RQPLTEVVTCLGDGHDGVWNLMEKIGVKRREILDWYHLVENMKKIGGSNKRLNRIKENLWKGEVKRVLEELEGCKKKQAINFTKYVDKHRERIPNYELYQ
SQGICIGSGSVESKIKQIGARMKIVGAQWKAENVPQYLKLRCAYLNGDIA
KCCLGAVAKVSFEDAEKAIKMATGMAVSGSSQQRLVQRYKFEEAEAKSPVEALSVEVGKVRIRTPKGQPSQGRDYKAVSLHGQECAGFFQQNEELLEWVN
RQPLTEVVTCLGDGHDGVWNLMEKIGVKRREILDWYHLVENMKKIGGSNKRLNRIKENLWKGEVKRVLEELEGCKKKQAINFTKYVDKHRERIPNYELYQ
SQGICIGSGSVESKIKQIGARMKIVGAQWKAENVPQYLKLRCAYLNGDIA
Blast result :
Comments
ISMae18 is 69% aa similar to ISMae42.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
The third ORF is the putative ORFAB reconstructed in silico by possible -1 frameshift.
References
1] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) Direct submission GenBank.
3] LIN, S., Stefan, H., Vingron, M., and Li, R.H. (2009) Direct submission.
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) Direct submission GenBank.
3] LIN, S., Stefan, H., Vingron, M., and Li, R.H. (2009) Direct submission.