ISMae42

  • Family ISKra4
  • Group ISMich2
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
AM778945.1 ND Microcystis aeruginosa
Microcystis aeruginosa PCC 7806
Microcystis aeruginosa NIES-843
DNA section
IS Length : 1196 bp

Ends


IR Length : 26/35

IRL : GGGAGCAAGTCAAAGCTGAAGAACAAATAAACTACTTGACAAAAGCATCA
IRR : GGGAGCAAGTCAGTTTTGCCAGAACATAAATACTATTCAATAGCTAAAGA

Insertion site


Left flankDirect repeatRight flankDR Length
CAGGATTTCAGATTACATCGTCT3
ACTCTAGTGCGATGTAATCG0
TTTCAAGGGATTCATCTCGTCCGTATTCA9
TATGAAAACAGCCTACTCCAGTGGTTGGT9
GCCATTAGCCGCATCAAAGTAAAGCCCAT9
ACATTTACTCGATTAAGATG0

DNA sequence

GGGAGCAAGTCAAAGCTGAAGAACAAATAAACTACTTGACAAAAGCATCAATTGGGCATGGGATAGAGAAAAGAGTGGGGAAAAGACAAAAATGACTCCA
GAGCAACAACAAGAACTTAACCAACATATTCAAGCGATAGCCAAGATTCTTCATCAGGAAGCTGAGGCTGAAAAAATCCAAACTTTAGAGGGAATAGAGA
CGACAATAAGAGAACAGACCTTAAAATATATAACTCCGAAACTAGGATTTTTTTTTGTCACAAAAACCACAGGAACTCAAGCCGGGAGACCGAGAAAAAT
AAAAAGCATCATCGGAGAATTATGCCTGACAGAAAAACAAGCAATCCGTTTAAATATTCCTCGTAATAACCAAATTAGTCCTTACTTAGAACGCTGTTGT
TTAAGAGCTAGTGCCAATGTTTCTTATGAAAATGCCGCCAGAGATATTCAATTTTATACAGGGATTAAGGTCTCGGCTAGGACTCAACAACGATTAGTCC
ATCGCCACCAATTTGCCGAAGAAGAATCAGGAAACCCCGTTCAAGAAATTAGTCTAGATGGAGAAAAAGTGCGCTTAAGAACCGAAACTAAGGGAGAGGC
TTGTATCTGGAAAGATTATAAAGCAATCTGTGTTGATACTTTCCTAAGAAAAGCTTGGTTTGGAGAGAATGAATCGTTAATATATTGGGTTAATCAACAA
CCTTTATCTGACCCCCTTACCTGTTTGGGAGACGGACATCCCGGTATTTGGAAAATTGTCAAAAACTGGGATTGTCCAGGAGAAAAAAGAGAAATACTTG
ACTGGTTTCATTTGGTAGAAAATCTTCATAAAGTCGGCGGTTCAGTGAAGAGATTGAAAAAAGCAGAAAGTTTATTATGGCAGGGTAAAATTGAGGAAAC
AATAGCATTAATCTCTCCTTTAAAAAACGAACGAGCTGAAAATTTCTGTCGTTATTTAGAGATTCATCGTCATGGAATTGTTAATTATAATTACTATCAA
CAAGAAGAGATTTGCTCTATTGCTTCGGGAGCCGTTGAATCCACGGTTAAACAGATTGACCGACGACTGAAAATTTCTGGAGCCCAGTGGAATGAAGAAA
ATATCCCTCAAGTGCTTAAACACCGCTGTGCTTACCTAAATAACAGTCTTTAGCTATTGAATAGTATTTATGTTCTGGCAAAACTGACTTGCTCCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1062 bp353 aa921153+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTPEQQQELNQHIQAIAKILHQEAEAEKIQTLEGIETTIREQTLKYITPKLGFFFVTKTTGTQAGRPRKIKSIIGELCLTEKQAIRLNIPRNNQISPYLE
RCCLRASANVSYENAARDIQFYTGIKVSARTQQRLVHRHQFAEEESGNPVQEISLDGEKVRLRTETKGEACIWKDYKAICVDTFLRKAWFGENESLIYWV
NQQPLSDPLTCLGDGHPGIWKIVKNWDCPGEKREILDWFHLVENLHKVGGSVKRLKKAESLLWQGKIEETIALISPLKNERAENFCRYLEIHRHGIVNYN
YYQQEEICSIASGAVESTVKQIDRRLKISGAQWNEENIPQVLKHRCAYLNNSL

 

Blast result :
Comments
References
1] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Frangeul,L. (2007) Direct submission GenBank.