ISAmo2
- Family ISLre2
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_018024 | ND | Anaerobaculum mobile | Anaerobaculum mobile DSM 13181 |
DNA section
IS Length : 1566 bp
Ends
IR Length : 23/28
IRL : GATCGCGTAAAATATTTGTAGGAGTTTTTTAGGTAAAAAATAAAGGGACC
IRR : GACCGCGTCAAGTTTTTGTAGGATTTTTAGGAACCACTCCCATGTATCTT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| CAACTTATTG | CTTTAACG | TCGTTTAATT | 8 |
| CGCCGAGTAG | GTAAACTTA | AGACCCCTTG | 9 |
DNA sequence
GATCGCGTAAAATATTTGTAGGAGTTTTTTAGGTAAAAAATAAAGGGACCGCTCCTTTTGGTAGAATCTTTGTGTGACCAAAAACTCATAAAGGAGGGTC
CCAACTTGAACAAAGTTATTTTACTACAAATTGTCTCAAACTTCATCTCCGAGATATTGAAGTTTTTTTGCAGTTCCAATGTCAGGACGCTGGCTGAAAT
AGAAGATGAGCTTTTTCGCATGACGAAGGCCTTCATCCGGGAGATCGTAAAGGCTTACCTTGAGCTTGCAGACGAAGCCATATTAAAGGACAAAACGTCT
CGAAAGCAAAGAGGATTGGTCGTAGAAAGGCGTGAGGACAGAAGGAGCTTATATACCATCTTTGGTGATATATCTTTCAAGAGGACCTACTACTTCGACA
AAAGTCATAAAAAATATGTATACCCCCTGGACGAAGCATTAAGGTTGGATAAACGTCAAAGGTTGAGCAAAACAGTAACGGTAAAGCTGGTAGAGACGGC
AGGCCAGGTATCTTACGCCAAAAGTTCGATGAACGTGACTGAGGGTGAGTTATCCAAGCAGACGGTAAAAAACAAGATCCACAGCCTAAAGCTGGAGGCC
TTAAAGACGAAAGTTCCTAAAAAAAGAGGCGTAGAGGTATTGCATATAGATGCAGATGAAGACCACGTTTCCCTACAGGAGGGCAGAAATGTGAATCTTC
CTTTAGTCTGCATATACGAGGGCACCTTTAAAGAGGGGTCCAAAAACAGATGTATAAACCCCATATACATGAGCGGTTACGGAAAGGACACAGATGAGTT
TTGGCTTGAGGTAGCAGATAGGATATACGAGCTTTACGACCCGGAGGACATAAAGGACATATACATCCATGGAGATGGTGCAAATTGGATAAGGCAAGGT
ATAAGCTGGCTGCCCGAGTCAAAGCTGGTGCTGGATAAATTTCACCTAAATAAAGCGATCCTTGAATCCACGGCAAGGCAGCCTGAAAAAAGACACGAAA
TATACAAAGCCATAAGGGAAAACGACATGAATTCCTTTAAAAGGATCATTCTAGATATGTTAAATGATGCCTCTGATGAAAAGGAAAGAAAAAGGATAGT
AGACTTCAGGAGGTATGTGACCAACAACTGGAACTCCATTACCATTCGAAACGAAGAGGACTGCGGCAGCTCAAGCTCCGAGGGTCACATTAGTCACGTG
CTTTCCTCTAGGTTAAGTTCCAGGCCTATGGCTTGGAGCAGGAAGGGCTTAAAGGCCATGTCTGCTTTAAGGGCTTACATCTGTAGTGGTGGGAAAGTGA
AAGCGGAACACGTAGGGGGAAACGACCAAAAGGGCGACAAGACAGACAAAGGACACAACTTCACATTAAACTTAGAAGATATCTTTAGTTCGGCTGCAAG
CGAGATAGGCTCCATAACAGTACTTAACATGGGGAAGGTAACGCCTTTATACACGACCTTAAAGGGCATATGTCATGGCGGGTTTGATTTTAGCTGAACT
AAAGACATTGCCCCTTAAGATACATGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
CCAACTTGAACAAAGTTATTTTACTACAAATTGTCTCAAACTTCATCTCCGAGATATTGAAGTTTTTTTGCAGTTCCAATGTCAGGACGCTGGCTGAAAT
AGAAGATGAGCTTTTTCGCATGACGAAGGCCTTCATCCGGGAGATCGTAAAGGCTTACCTTGAGCTTGCAGACGAAGCCATATTAAAGGACAAAACGTCT
CGAAAGCAAAGAGGATTGGTCGTAGAAAGGCGTGAGGACAGAAGGAGCTTATATACCATCTTTGGTGATATATCTTTCAAGAGGACCTACTACTTCGACA
AAAGTCATAAAAAATATGTATACCCCCTGGACGAAGCATTAAGGTTGGATAAACGTCAAAGGTTGAGCAAAACAGTAACGGTAAAGCTGGTAGAGACGGC
AGGCCAGGTATCTTACGCCAAAAGTTCGATGAACGTGACTGAGGGTGAGTTATCCAAGCAGACGGTAAAAAACAAGATCCACAGCCTAAAGCTGGAGGCC
TTAAAGACGAAAGTTCCTAAAAAAAGAGGCGTAGAGGTATTGCATATAGATGCAGATGAAGACCACGTTTCCCTACAGGAGGGCAGAAATGTGAATCTTC
CTTTAGTCTGCATATACGAGGGCACCTTTAAAGAGGGGTCCAAAAACAGATGTATAAACCCCATATACATGAGCGGTTACGGAAAGGACACAGATGAGTT
TTGGCTTGAGGTAGCAGATAGGATATACGAGCTTTACGACCCGGAGGACATAAAGGACATATACATCCATGGAGATGGTGCAAATTGGATAAGGCAAGGT
ATAAGCTGGCTGCCCGAGTCAAAGCTGGTGCTGGATAAATTTCACCTAAATAAAGCGATCCTTGAATCCACGGCAAGGCAGCCTGAAAAAAGACACGAAA
TATACAAAGCCATAAGGGAAAACGACATGAATTCCTTTAAAAGGATCATTCTAGATATGTTAAATGATGCCTCTGATGAAAAGGAAAGAAAAAGGATAGT
AGACTTCAGGAGGTATGTGACCAACAACTGGAACTCCATTACCATTCGAAACGAAGAGGACTGCGGCAGCTCAAGCTCCGAGGGTCACATTAGTCACGTG
CTTTCCTCTAGGTTAAGTTCCAGGCCTATGGCTTGGAGCAGGAAGGGCTTAAAGGCCATGTCTGCTTTAAGGGCTTACATCTGTAGTGGTGGGAAAGTGA
AAGCGGAACACGTAGGGGGAAACGACCAAAAGGGCGACAAGACAGACAAAGGACACAACTTCACATTAAACTTAGAAGATATCTTTAGTTCGGCTGCAAG
CGAGATAGGCTCCATAACAGTACTTAACATGGGGAAGGTAACGCCTTTATACACGACCTTAAAGGGCATATGTCATGGCGGGTTTGATTTTAGCTGAACT
AAAGACATTGCCCCTTAAGATACATGGGAGTGGTTCCTAAAAATCCTACAAAAACTTGACGCGGTC
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1392 bp | 463 aa | 106 | 1497 | + | No |
Chemistry : DDE
ORF sequence :
MNKVILLQIVSNFISEILKFFCSSNVRTLAEIEDELFRMTKAFIREIVKAYLELADEAILKDKTSRKQRGLVVERREDRRSLYTIFGDISFKRTYYFDKS
HKKYVYPLDEALRLDKRQRLSKTVTVKLVETAGQVSYAKSSMNVTEGELSKQTVKNKIHSLKLEALKTKVPKKRGVEVLHIDADEDHVSLQEGRNVNLPL
VCIYEGTFKEGSKNRCINPIYMSGYGKDTDEFWLEVADRIYELYDPEDIKDIYIHGDGANWIRQGISWLPESKLVLDKFHLNKAILESTARQPEKRHEIY
KAIRENDMNSFKRIILDMLNDASDEKERKRIVDFRRYVTNNWNSITIRNEEDCGSSSSEGHISHVLSSRLSSRPMAWSRKGLKAMSALRAYICSGGKVKA
EHVGGNDQKGDKTDKGHNFTLNLEDIFSSAASEIGSITVLNMGKVTPLYTTLKGICHGGFDFS
HKKYVYPLDEALRLDKRQRLSKTVTVKLVETAGQVSYAKSSMNVTEGELSKQTVKNKIHSLKLEALKTKVPKKRGVEVLHIDADEDHVSLQEGRNVNLPL
VCIYEGTFKEGSKNRCINPIYMSGYGKDTDEFWLEVADRIYELYDPEDIKDIYIHGDGANWIRQGISWLPESKLVLDKFHLNKAILESTARQPEKRHEIY
KAIRENDMNSFKRIILDMLNDASDEKERKRIVDFRRYVTNNWNSITIRNEEDCGSSSSEGHISHVLSSRLSSRPMAWSRKGLKAMSALRAYICSGGKVKA
EHVGGNDQKGDKTDKGHNFTLNLEDIFSSAASEIGSITVLNMGKVTPLYTTLKGICHGGFDFS
Blast result :
Comments
ISAmo2 is 91% aa similar to ISAmo1.
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
IS of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).