ISCde3

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010424 ND Candidatus Desulforudis
Candidatus Desulforudis audaxviator MP104C
DNA section
IS Length : 1572 bp

Ends


IR Length : 18/28

IRL : GTGAGTGTAAGGTTTAAGTAGGAGATGTGAGGTCGGAAATAGAAGTGAGA
IRR : GTCCGTGTCAAGACTTAGTAGGCGATCTCCCAGCCGATTTAATTTGAATT

Insertion site


Left flankDirect repeatRight flankDR Length
GAGGCTGTGCAATTAACCAGTTCCGCTTG9
AAGGATTGAAGCGCGGGCGGGTTCGCTTG9
GATATTTTGGCCGAATCCGCAGTGCTTTG9
GCAACGCTTATTCTTCGGTACCGGCGTTG9
GCGGCCGGGGCCATGCCCTTTCGGCATTC9
CGCCCAGCCGGACCTGGTACCCCATCTTG9
TTTCGGGGTTTTGCATTGACAGCACGTGG9
TGAACAGCAGTGAGCCCGGTAGCACCTTG9
AGGAAGGTGGCTTTTCAATT0
GGAGGAGGTTCTGGAGGTTC0

DNA sequence

GTGAGTGTAAGGTTTAAGTAGGAGATGTGAGGTCGGAAATAGAAGTGAGACCTCAAAACGGCTTCCCCTGAGGGGAAGCCCTCCCCGCCAAAGGAGTGAG
GTCTCATGTTCAAGATTCGCTTTCTGCTTGAGGTAGTCCTGTTTCTGGCCAAGGGAATTTTTGAGGTGTTCAGGTCGGTGCGCAATATGGATGAGTTGGA
AGAGCGGGTGCAGCGGTTGGTTCAACAGGCAGGCGGCAAAATGCTGGAGGAGGCGCTGGCACACATTGACCTTGAGTTAAGCGGCAAGCGGGATCCAGCC
CTCAAGAACGTGGGACAGCGGTCGCGGACCCTGGTCACCAGCTTCGGTGAGATTACGGTTAAGCGCCGACTGTACCGTAACCAGAAGACCGGCGCGTACC
GGTTCCTTCTGGACGAAGCCTTGGGAATCCCTGAACGCCAACGGGTGACACCGCGGATGACCCGTATGATCCTGGAGCTAGGCACGGAGATGCCCTTCAG
GCGCGCGGCCCGGGTTATGGGTTTTCTAGTACCGGGGATTCACTGGATGACCGTCTGGTCTAAGGTTCAGGATGCTGGAGAAAAGGCCGCCCGGGATGCT
GAGGCACTACGCGAGGCCGTCTTTGAAGACGGTGTGGTCCCTGAAGGCGGCAAGGAGGTTCGGGAGTTATCCATTGAAGCCGATGGTGTAGTAATACCCT
TGCAGCGGTCGCCCAAGGCGTTTGGTGAGATCAAGCTGTTTATCGGCTACGAGGGCAGGGAACAAAAGACCCGGAAACTGGTGAACCGTTACACGGTGGC
CACCGCCAGGGGCAGCCGGGTGGCTTGGGAAGACACCGGGGCGGCCTTCGGCCACAAGTGGGACCTTAGCAGGGTAGAGCGGATCCGCATTGGTGGGGAC
GGCGCCGAGTGGATCAAACAAGGCCTGGAGATGTTCCCCGGGGCGACTTACCACCTTGACCCCTTCCACCTGCGCCGGCGTTTAACCGAGGCTTTAAGTT
ACAGCAGCAACGTCTATGAAACTGTCACCGAAGGGTTAGCCGAGCTAAACCAAGATGCGGTAGTTTCCGCCTTGGACCAGGCGATTCGGGTCAACCGGGG
TGCACGCCGTAACGGGATCATGCGGCTTAAGGAGTATCTCCTGGCTAACTGGCAAGGTATCGCCGCCTTGCCGGAGGGAGACCGTTTAGGTGCGATCGAG
GGCCAGGTCCGTCACACCATCGCCAGGCGAACAAAGCGGATTGGGGCGCGTTGGAGCCCGGCCGGTGCGGAACGGATGGGACGCCTGTTAGCGGTACGGG
CTAATGATGAGCTGGACAGATACGTGGTACACTCGGAACCCCGGTTCGACCTACTGAAAAAGGCTGTCGGAGCCGAGGCAATCCAACTGCCCAAACGCTT
CGGCAAAGATCCCGAGGCCTGGCTTCAAGCCAACGTGCCCGCCCTTGAAGGACCGCACGCCGGAAAACACTGGGTCAAGCACATAGTAAGAGAGATTAGT
TCACCGAGGTGGTCTACGGTCTAATTCAAATTAAATCGGCTGGGAGATCGCCTACTAAGTCTTGACACGGAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1419 bp472 aa1061524+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MFKIRFLLEVVLFLAKGIFEVFRSVRNMDELEERVQRLVQQAGGKMLEEALAHIDLELSGKRDPALKNVGQRSRTLVTSFGEITVKRRLYRNQKTGAYRF
LLDEALGIPERQRVTPRMTRMILELGTEMPFRRAARVMGFLVPGIHWMTVWSKVQDAGEKAARDAEALREAVFEDGVVPEGGKEVRELSIEADGVVIPLQ
RSPKAFGEIKLFIGYEGREQKTRKLVNRYTVATARGSRVAWEDTGAAFGHKWDLSRVERIRIGGDGAEWIKQGLEMFPGATYHLDPFHLRRRLTEALSYS
SNVYETVTEGLAELNQDAVVSALDQAIRVNRGARRNGIMRLKEYLLANWQGIAALPEGDRLGAIEGQVRHTIARRTKRIGARWSPAGAERMGRLLAVRAN
DELDRYVVHSEPRFDLLKKAVGAEAIQLPKRFGKDPEAWLQANVPALEGPHAGKHWVKHIVREISSPRWSTV

 

Blast result :
Comments
ISCde3 is 50% aa similar to ISHor2.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
[1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
[2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
[3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
[4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).