ISClsp4

  • Family ISLre2
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_015737 ND Clostridium sp.
Clostridium sp. SY8519
DNA section
IS Length : 1787 bp

Ends


IR Length : 17/25

IRL : CATAGAGTAAAATTTTAGTTGGCAAAAACAAAAAGAAGAGGCCGAAGCCC
IRR : GATACTGTCAAGTAATCGTTGGCAACCCACTCATACATTTCAACCGTACG

Insertion site


Left flankDirect repeatRight flankDR Length
TTTCGTTTGTCATAGGCGGAAACCCACTA9
GAGTGTCACTGTGTAACCTTTTTCCAGGA9

DNA sequence

CATAGAGTAAAATTTTAGTTGGCAAAAACAAAAAGAAGAGGCCGAAGCCCCTTCCATATCATACAGAAAACCATTAGTATTTATTTTGCTAAGAATACAT
ACGAAAGGATCTGTATGATGAATACTATGATAGAGGAAAAGGGCGTAACTTTCAAGGAATTAGAGCAGGAGATTTTCAGATTTGTTTGCGACCTTGGCGT
CGCACTGACCCAAGAGATACTCCAGCAGTATGATAAATACCTGCATGACAGCAGAGATACATCCGAACTCCGTGACAAAGGGACGCGCCACACATCTATC
AAAACGGTCTATGGAGACGTGCCTTATGACAGGTATGTGTACAGGACCAGGGATGAGTATGGTGAAAATCACTATGTCTATCTGTTGGATGAAAATCTGA
AGATGAACCGTGTCGGCCTGGTTTCAGAGAATTGTGTGGAACTCATGGTTTCATCCATCACAGAGATGTCCTATCGGAATTGTGCCAACAAAGTTTCCGA
CATGACAGGGCTCCCCATCAGCCATACCGGTGTGTGGAATATCATACAGACTCTTGGAGAAAAACTGGAAAATGATGAGAGGGAACTTGTTGCAGCTAAT
AAGAAACAAGAAGTAAAAGGCGAAAAAGAAGTTCCAGTGTTGTTTGAAGAAGCTGATGGTGTATGGCTGAATCTTCAGGGAAAAGATCGAGAAAAACGGC
ATTTCCCAAAAGCGGAAATGAAAGCAGCAATCGCCTATGATGGTTGGAAAGCGGAGGGAAACGACCGCTACTCTCTTGATGGAAAAGTTGTGACTGCCGG
ATTTGAGAAGGCTTCCGAATTCCAGAAAAAAAGAGAGGCGATGATCGCTGCCGAGTATAACCTCGATGAGGTTCAGTTACGCTTCCTGAATGGTGACGGA
GCTTCCTGGATCAAGAAAACGCAGGATAAGGAAACGGTGTTCCAGCTGGATCCGTTTCACAGAAACCAGAGTATCCGGAAAAACCTGCCCCATGCAAAAG
CAATCCATGATGTTATGGAAATGCTGCGTGAGAATAAGGTGGCGGAAACTTTTGCTTATCTTGAAATATATCGTGACAGCCTGGACGATGATGAATCCAT
AAAGAAGGTGGAAGAAGTGATCAGCTACTTCCGTTCCAACAGGGATGGCCTACTGCCATATCAGGAGCGTGGGCTGGAAATCCCGGAAAGCCCGGAAGGA
CTTATTTATAGAAAAATGGGGACGATGGAGAACCATATCTGGAGCGTCATAGCCAGGAGAATGAAGCATAACCATACCAGCTGGAGCATCAAGGGCGGTA
ACCATCTGGCAAAGATCCTGGCGAAGAAATGTTCCGGAAAGCTTTATGAAGTAACGGAGCAGCTGAGAATACCGATATTTGAAAAAGAGAAACTGGAAGA
AATCAAAGGAGATATCCTGCAGGCAGGCCAGATCAGGAAGAAGATCGGAAAGGGATACGAATACCCGGTTATTGGCCATATGACTGCGTTAGAAGGGAAG
ACATCCGGGGAACGGGGAAAACTTTTGGCCATGGCAGGATTTTAAACAGTTCCGGAAGTTATAATGGTGTGCAATGAAAAACGATAGCGAGGCTTTCGTA
GGGAGCTGCATGCTAGCAGCGAAGGCGGGCGCATGTTTGAGCCCAAAGGGCGAGTTCGCGCAGGAGCCTGGGCGAAAAAGCGCATGCAGCGAGCAGAAAA
CACAGCGTGTTTTCATACACACCTTATAAAGACGGAACGTACGGTTGAAATGTATGAGTGGGTTGCCAACGATTACTTGACAGTATC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1428 bp475 aa1181545+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MNTMIEEKGVTFKELEQEIFRFVCDLGVALTQEILQQYDKYLHDSRDTSELRDKGTRHTSIKTVYGDVPYDRYVYRTRDEYGENHYVYLLDENLKMNRVG
LVSENCVELMVSSITEMSYRNCANKVSDMTGLPISHTGVWNIIQTLGEKLENDERELVAANKKQEVKGEKEVPVLFEEADGVWLNLQGKDREKRHFPKAE
MKAAIAYDGWKAEGNDRYSLDGKVVTAGFEKASEFQKKREAMIAAEYNLDEVQLRFLNGDGASWIKKTQDKETVFQLDPFHRNQSIRKNLPHAKAIHDVM
EMLRENKVAETFAYLEIYRDSLDDDESIKKVEEVISYFRSNRDGLLPYQERGLEIPESPEGLIYRKMGTMENHIWSVIARRMKHNHTSWSIKGGNHLAKI
LAKKCSGKLYEVTEQLRIPIFEKEKLEEIKGDILQAGQIRKKIGKGYEYPVIGHMTALEGKTSGERGKLLAMAGF

 

Blast result :
Comments
ISClsp4 is 50% aa similar to ISHor2.
ISs of ISLre2 family preferentially insert in intergenic region, 15 to 17 bp upstream the -35 box of sigmaA promoters. [2][3][4]
References
1] Romain Guérillot and Philippe Glaser (2013) Direct submission.
2] Brochet M, Da Cunha V, Couvé E, Rusniok C, Trieu-Cuot P, and Glaser P, P.(2009) Mol Microbiol. 71(4):948-59;
3] Guérillot R, Da Cunha V, Sauvage E, Bouchier C, Glaser P, (2013) Modular evolution of TnGBSs, a new family of ICEs associating IS transposition, plasmid replication and conjugation for their spreading (submitted)
4] Guérillot R, Glaser P, (2013) Diversity of bacterial prokaryotic mutator like transposase, association with conjugation conjugative elements and insertion specificity (on preparation).