ISMco2

  • Family IS200/IS605
  • Group IS200
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_015416 ND Methanosaeta concilii
Methanosaeta concilii GP6
DNA section
IS Length : 589 bp

Ends


Left end : ATTTTTAAATGAATACCCCGCAGCTTGCTGCGGTTGGGATGGTCAGGAAACCGACTTAAGGAAAGGGGCATATTCTATGTTAGATGATTGAGGTTAAAAA II struct. : Yes
Right end : AAGAAAAGGAAGCATATAAGCAAATGAACTTGTTAGATTTCGAATGACGGCAAACGGCGCGAACATGCCCCGCTGCTTGCAGCGGGGTGCGCTGTTTGAC II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TTTAAGCATTTTAGTATTTTTGAATCCTGGATGAC
TCCCTTTCCCCGCATATTTTTATAAAAACAGTGAC

DNA sequence

ATTTTTAAATGAATACCCCGCAGCTTGCTGCGGTTGGGATGGTCAGGAAACCGACTTAAGGAAAGGGGCATATTCTATGTTAGATGATTGAGGTTAAAAA
AGCAAGCCATTGTGCCTACAAAATTCGATATCATATGGTATTTAGTATAAAATATAGAAGAAAATTGCTTCAGGATATCGATCGCATTAATTATATCAAA
TTCGTATGCAGTGAGATCGGTGAACGATACTATTTCGATTTCGATGCAATTGGTACTGATGGCGATCATGTTCATATCTTTGTTGGAGCAGCGCCAAGAT
ATTCGCCATCAAAGGTTATGCAAATTGTAAAAAGTATAACCGCAAGAGAGTTTTTTAAGAAGTATCCTGAAGTGAAAAAACAGCTTTGGGGTGGCGAATT
TTGGAGCGATGGAGGTTATGTTGGAACAGTAGGTGATGGTGTTATGGCCGACACTATACGGAATTACGTTGAAACTCAAGGATCGCCTGAAGAAAAGGAA
GCATATAAGCAAATGAACTTGTTAGATTTCGAATGACGGCAAACGGCGCGAACATGCCCCGCTGCTTGCAGCGGGGTGCGCTGTTTGAC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
453 bp150 aa84536+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MIEVKKASHCAYKIRYHMVFSIKYRRKLLQDIDRINYIKFVCSEIGERYYFDFDAIGTDGDHVHIFVGAAPRYSPSKVMQIVKSITAREFFKKYPEVKKQ
LWGGEFWSDGGYVGTVGDGVMADTIRNYVETQGSPEEKEAYKQMNLLDFE

 

Blast result :
Comments
ISMco2 is 85% aa similar to ISMba18.
References
1] ISfinder annotation (20121)
2] Barber,R.D., Zhang,L., Harnack,M., Olson,M.V., Kaul,R., Ingram-Smith,C. and Smith,K.S. (2011) J. Bacteriol. 193 (14), 3668-3669.