ISfinder

ISMev1

Family IS5
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_014253	ND	Methanohalobium evestigatum	Methanohalobium evestigatum Z-7303

DNA section

IS Length : 1034 bp

Ends

IR Length : 13

IRL : GGCTCTGTAGAAAATCTGATTTTTGATTTGATTTACTACTGTGATGTAAG
IRR : GGCTCTGTAGAAATCCTCAAATAAACAATAATTTTAACCTTGACAAATCT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
ATAAGGCTAATTT		AAATTATTACTTT	0

DNA sequence

GGCTCTGTAGAAAATCTGATTTTTGATTTGATTTACTACTGTGATGTAAGAGTTTTAAAGGGATGACCCCCTGAGTTATTATTTACCACTAAAATAACAA
GGGGGCCGATGTGTTTGTGCAATAAGCACACAGGTAATATTTCATCTAATAAGTACTTAAACTTTATCGATACAGCTTTAGCTGTATCGGGCAACTCAAG
ACTTGAAATCTACAGCTGCAAGTATTCCAAGAGAACCTATACACAGCACCAGTTGTTGGCGCTGGTGCTGCTCAAAGAATACCTCAACGAGGATTACAGG
GATATCGTTGAACTTGTAGAGTTGATGGACAAAGTCCAGACTAAACTCGGTCTCAAACAGATACCCCATTTCACAACCCTCCATAAATTCGTGACCAGAA
TTAGAACTATCCTCCTCAACAGACTGTTACATCAAACAATCAAACTCTTTTATTCTCATGGTGAAAGAATCGAGGTCACTGCCGTGGATTCTACGGGGTT
CACGAGTGGACACTGCAGTTACTATTACTCGTTAAGAACCGATAAGAAACGACATTCATTCCTGAAAACTAGTATCGCTGTGGATACCAGTAATTTGGTT
ATTATCGGTGTCAAGGTCTCTGGTAGACCAGTACATGATACTAAACATGCAGTGACATTGTTGAAACAATGTCATAGAATCCGGAAATCGGAATTCTATG
TCATGGATAAAGCCTATGACTCGGAAGATATCCATAAACTGGTAAGAGATGAACTGGGTTCCATTGCTATAATTCCTGTAAAGGATAGAAAACGCAAGAG
GATCAAGGGTCGATACCGAAGGTTAATGATTGAGGATTTTGATGTGGCTTTGCATCGTAAAAGGAGCATGTCTGAGACTTCTAATTCTGTACTAAAACGC
AAATATGGTGAAGAAATCAGAGCAAGAATATACCGAAACCAAGTTAAAGAGATAATTTTAAAGGTTGTTGTATACAACCTTGACAGATTTGTCAAGGTTA
AAATTATTGTTTATTTGAGGATTTCTACAGAGCC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
926 bp	308 aa	109	1034	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MCLCNKHTGNISSNKYLNFIDTALAVSGNSRLEIYSCKYSKRTYTQHQLLALVLLKEYLNEDYRDIVELVELMDKVQTKLGLKQIPHFTTLHKFVTRIRT
ILLNRLLHQTIKLFYSHGERIEVTAVDSTGFTSGHCSYYYSLRTDKKRHSFLKTSIAVDTSNLVIIGVKVSGRPVHDTKHAVTLLKQCHRIRKSEFYVMD
KAYDSEDIHKLVRDELGSIAIIPVKDRKRKRIKGRYRRLMIEDFDVALHRKRSMSETSNSVLKRKYGEEIRARIYRNQVKEIILKVVVYNLDRFVKVKII
VYLRISTE

Blast result :

Comments

ISMev1 is 79% aa similar to ISMbu10. There is no STOP codon inside the insertion sequence, the transposase use the first after the end of the IS.

References

1] ISfinder annotation (2010)
2] Lucas,S., Copeland,A., Lapidus,A., Cheng,J.-F., Bruce,D., Goodwin,L., Pitluck,S., Saunders,E., Detter,J.C., Han,C., Tapia,R., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Sieprawska-Lupa,M., Whitman,W.B., Anderson,I. and Woyke,T. (2010) Direct submission GenBank.