ISfinder

ISMth3

Family IS5
Group IS903

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008553	ND	Methanosaeta thermophila	Methanosaeta thermophila PT

DNA section

IS Length : 988 bp

Ends

IR Length : 17/18

IRL : TGCTGTGTTGGATAACTATTTAAACTAGGAAAATATATAATATATTTCAT
IRR : TGCTGTGTTGGATAAGTAGCTTTATATTCATATATTCTCTCTTATTCTTT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GATGTTTTTG	CATAGAGT	TGATAGTAGG	8

DNA sequence

TGCTGTGTTGGATAACTATTTAAACTAGGAAAATATATAATATATTTCATGGCCTATGAGGATAACCGCAATTGGCGCGAATACAATGAGAAATTGGTTA
GGCGAGGATGGTTTTACCTTAGCACTGACTTTGTGAATAATTGGGATGAAGAGCTACTGAAGATGAATAAGAACAAGAATGGCAGACCCTATCGCTATCC
TGAGACATTTATTCAATTTTGTGGTTTAGCATACGCCTTTCTTCATTTACCATACAGGCAGCTCGAAGGATTTATTCAGGCGCTAAGCGGATTTGTTCCT
GGGCTGTTGGCTGCCGATTATTCGACATTATGGCAGAGGATTACGAATTTGGAGTTGAATATTCCAATACCTGATAACGATTTAGTGGTCGCAGTTGACT
CAACAGGAATGAAGGTTACGAATAGAGGCGACTGGATGAGAGAAAGTCATGGTGTTGAACGCAGAGGCTGGATAAAAGTGCATATCGCCGTAGATGTTGA
AACAAGGAAGCCCGTAACCTTCGAGATAACCGATGAGACCGTCACTGATCATGAGATGGTAAAACCGCTGCTGGAAGATGTTAAGCTTGAAGATTCACTG
ATGGATGGAGCTTATGATAAGGAGGGGGTATTCGATTTCATGAAAGAGAAGGGCGTAGATATGCCTGGAATCAAGATCAGGAAGAATGCTATCGTCAAAG
CAGGCTCGTCCAGAGCCGAACCAGTTCTTGAGTTTATGAAGTATGGATACCACAGTTGGAAAATTGTGCATGGATATGGAAGAAGGTGGGCGGCTGAAAG
TGTATTCTCAGCAATTAAGAGGATATTTGGCGAGACTGTGAGGGCCACTTCGAAGGAAGGCATGATTCGCGAAGTACGCAGGATGTTCACATTTTATACT
ATAATTTTAAGCGTATAACATTATGACGAAGATTCTTGAAAGAATAAGAGAGAATATATGAATATAAAGCTACTTATCCAACACAGCA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
870 bp	289 aa	49	918	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MAYEDNRNWREYNEKLVRRGWFYLSTDFVNNWDEELLKMNKNKNGRPYRYPETFIQFCGLAYAFLHLPYRQLEGFIQALSGFVPGLLAADYSTLWQRITN
LELNIPIPDNDLVVAVDSTGMKVTNRGDWMRESHGVERRGWIKVHIAVDVETRKPVTFEITDETVTDHEMVKPLLEDVKLEDSLMDGAYDKEGVFDFMKE
KGVDMPGIKIRKNAIVKAGSSRAEPVLEFMKYGYHSWKIVHGYGRRWAAESVFSAIKRIFGETVRATSKEGMIREVRRMFTFYTIILSV

Blast result :

Comments

ISMth3 is 53% aa similar to ISFac2.

References

1] ISfinder annotation (2010)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E., Smith,K.S., Ingram-Smith,C. and Richardson,P. (2006) Direct submission GenBank.