ISMra6
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010518 | ND | Methylobacterium radiotolerans | Methylobacterium radiotolerans JCM 2831 plasmid pMRAD05 |
DNA section
IS Length : 851 bp
Ends
IR Length : 16/17
IRL : GAGCGTCTAACAAAACCGATGGCGGCGCGTTGAGAGGAGATGGCGAGACC
IRR : GAGCCTCTAACAAAACCAGCGTTCGATGAAGCGCCAGACGATTAGGGCTG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
AAACCGGAGC | TAA | GTCGATCGAC | 3 |
DNA sequence
GAGCGTCTAACAAAACCGATGGCGGCGCGTTGAGAGGAGATGGCGAGACCACTCCTTCCCGATGGCCTGTGGGCTGAGATCGCACCGCTGCTGCCGCCTG
AGCCAGCCAAGCCGAAGGGCGGTCGCCCGCGAAGATCTGACCGCGCGGCGTTGACGGGCATCCTGTTCGTGCTCCGCTCAGGCACGCCGTGGGAGTTGCT
TCCGCGCGAGATGGGCTGTGGCTCCGGCATGACCTGCTGGCGACGCTTGCGCGATTGGCAGAAGGCCGGGGTTTGGGACCGCCTGCAACAGACGCTGCTC
GATCGGCTCGGACGCCGGAACGGCATCGACTTCAGCCGCGCCTCCCTCGACAGCGCCTCCATCGCTGCAAAAAAGGGGGCCCGGCGTCAGGCCCGAACCC
GACCGACCGGGGCAAGCCGGGCACGAAGCGTCACGTCCTCACCGATGCGAAGGGCATCCCGCTCGCGCTGAAGCTGACGGGGGCCAACGTGCACGACAGC
CGGATGCTCGAGGCGGTGGTGGATGCTGTGCCGCCGATCCGCCAGTGCTGGGGACGACCGCGCAAACGCCCGTCCCGGTTGCACGCCGACAAGGCCTACG
ATCACCGCCGCTGCCGTCAGGCGCTCACCCGCCGTCGCATCCAGCACCGCATCGCCCGTCGCGGCATCGAGAGCAGCCAACGTCTGGGCCGGCACAGGTG
GGTCGTGGAGCGCACGCTGGCTTGGTTCGCCCAGTTCCGCCGCCTCGCCATCTGCTACGAGCGACGGACCGACATCCACCTCGCCTTCTCCACCCTGGCC
GCAGCCCTAATCGTCTGGCGCTTCATCGAACGCTGGTTTTGTTAGAGGCTC
AGCCAGCCAAGCCGAAGGGCGGTCGCCCGCGAAGATCTGACCGCGCGGCGTTGACGGGCATCCTGTTCGTGCTCCGCTCAGGCACGCCGTGGGAGTTGCT
TCCGCGCGAGATGGGCTGTGGCTCCGGCATGACCTGCTGGCGACGCTTGCGCGATTGGCAGAAGGCCGGGGTTTGGGACCGCCTGCAACAGACGCTGCTC
GATCGGCTCGGACGCCGGAACGGCATCGACTTCAGCCGCGCCTCCCTCGACAGCGCCTCCATCGCTGCAAAAAAGGGGGCCCGGCGTCAGGCCCGAACCC
GACCGACCGGGGCAAGCCGGGCACGAAGCGTCACGTCCTCACCGATGCGAAGGGCATCCCGCTCGCGCTGAAGCTGACGGGGGCCAACGTGCACGACAGC
CGGATGCTCGAGGCGGTGGTGGATGCTGTGCCGCCGATCCGCCAGTGCTGGGGACGACCGCGCAAACGCCCGTCCCGGTTGCACGCCGACAAGGCCTACG
ATCACCGCCGCTGCCGTCAGGCGCTCACCCGCCGTCGCATCCAGCACCGCATCGCCCGTCGCGGCATCGAGAGCAGCCAACGTCTGGGCCGGCACAGGTG
GGTCGTGGAGCGCACGCTGGCTTGGTTCGCCCAGTTCCGCCGCCTCGCCATCTGCTACGAGCGACGGACCGACATCCACCTCGCCTTCTCCACCCTGGCC
GCAGCCCTAATCGTCTGGCGCTTCATCGAACGCTGGTTTTGTTAGAGGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
432 bp | 143 aa | 40 | 471 | + | No |
Description : First part of the transposase
ORF sequence :
MARPLLPDGLWAEIAPLLPPEPAKPKGGRPRRSDRAALTGILFVLRSGTPWELLPREMGCGSGMTCWRRLRDWQKAGVWDRLQQTLLDRLGRRNGIDFSR
ASLDSASIAAKKGARRQARTRPTGASRARSVTSSPMRRASRSR
ASLDSASIAAKKGARRQARTRPTGASRARSVTSSPMRRASRSR
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
603 bp | 200 aa | 243 | 845 | + | No |
Description : Second part of the transposase
ORF sequence :
TLARLAEGRGLGPPATDAARSARTPERHRLQPRLPRQRLHRCKKGGPASGPNPTDRGKPGTKRHVLTDAKGIPLALKLTGANVHDSRMLEAVVDAVPPIR
QCWGRPRKRPSRLHADKAYDHRRCRQALTRRRIQHRIARRGIESSQRLGRHRWVVERTLAWFAQFRRLAICYERRTDIHLAFSTLAAALIVWRFIERWFC
QCWGRPRKRPSRLHADKAYDHRRCRQALTRRRIQHRIARRGIESSQRLGRHRWVVERTLAWFAQFRRLAICYERRTDIHLAFSTLAAALIVWRFIERWFC
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
806 bp | 268 aa | 40 | 845 | + | Yes |
Chemistry : DDE
ORF sequence :
MARPLLPDGLWAEIAPLLPPEPAKPKGGRPRRSDRAALTGILFVLRSGTPWELLPREMGCGSGMTCWRRLRDWQKAGVWDRLQQTLLDRLGRRNGIDFSR
ASLDSASIAAKKGGPASGPNPTDRGKPGTKRHVLTDAKGIPLALKLTGANVHDSRMLEAVVDAVPPIRQCWGRPRKRPSRLHADKAYDHRRCRQALTRRR
IQHRIARRGIESSQRLGRHRWVVERTLAWFAQFRRLAICYERRTDIHLAFSTLAAALIVWRFIERWFC
ASLDSASIAAKKGGPASGPNPTDRGKPGTKRHVLTDAKGIPLALKLTGANVHDSRMLEAVVDAVPPIRQCWGRPRKRPSRLHADKAYDHRRCRQALTRRR
IQHRIARRGIESSQRLGRHRWVVERTLAWFAQFRRLAICYERRTDIHLAFSTLAAALIVWRFIERWFC
Blast result :
Comments
ISMra6 is 83% aa similar to ISAzba7.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] ISfinder annotation (2010).
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C.J. and Richardson,P. (2008) Direct submission GenBank
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C.J. and Richardson,P. (2008) Direct submission GenBank