ISMae39

  • Family IS30
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_010296 ND Microcystis aeruginosa
Microcystis aeruginosa NIES-843
DNA section
IS Length : 1092 bp

Ends


IR Length : 20/26

IRL : CGCCAATTCAAAAATGAAACGCATCAACCTCAAGAGTTGACAAAGAAAGG
IRR : GGCCAATTCAAGTCTGAATTGCATCACTGTCTGATCTACCTTTATAGAAT

Insertion site


Left flankDirect repeatRight flankDR Length
TACTTTTTTTCTCAAGTCTAGGC3
TTTGATCAAGAAACTAAACCAAA3
ATTGCTTTTTATCTCTAGGTTGT3
GAACCGTAGAAATTAAGTGCAGA3
CCTATAATTATACTATCGCCGGT3
AACTATATTATTGTCCTTGAGTA3
CCCCAAAGTTTTGAAAGGTT0
TTAGATGCTGGTTTACTGTT0

DNA sequence

CGCCAATTCAAAAATGAAACGCATCAACCTCAAGAGTTGACAAAGAAAGGCTCATCAGGTACTGTCTGATCATTAACAAAAACAAATCAGACCCAAAAAT
GAGCCACCAACATCTTAACATAGAACAAAGAAACTTGCTCTACCAACTTAGTCAGGAGGGAAATTTATCTCAACGGCAAATGGCCGTCTGGCTCGGATGT
CATCAAAGCACAATATCACGGGAATTAAAAAGGAATCAAAGTTCCCTTGGCTGCTATCTACCTGACACAGCACAAGCTCAAAGCGAGACAAGGCGAAAAA
ATGCCAAACAACCCTTTAAAAATGTCAGCGAGTCAGCCTTAGAGTTGGTCAAAGAAGGATTGAAAGATTATCATAGTCCCGAACAAATAGCAGGTCGTCT
GAAAAGAGCCAGTCAAGAATCTCTCAGTCACGAAACCATCTATCAAATGATCTATCAGAACTATCACGGATGTGGAGAGTACCAGAAATATTTACGCCGG
GGGCTTTGTCAAAGAAAGAGTCGAGGCGGAGCAAAAAGTAAGCGTGGAGGGATTCCAGGGCGTGTAGATATCAGCGAGCGACCAGTAATTGCTGACCAGA
AAACTGAAATCGGTCATTGGGAAAGTGACACAATGATTGGAGGCAATCATCTAGGAGTTCTCGTTACTCATGTAGACAAGGCCTCGAAATTTCTAGTAGC
AGGATTAGCCAAAAACAAAACCGCATCGGAAATCAATAGAGTCACAGAAAAACTTTTTCAAGAGATTCACCCTGACAAAAGAAAAACCTTTACTTGTGAC
AACGGCAAAGAATTTTGTGGACATCAAGAGTTGAGTCAGAAATTGGGAGCAGATTTTTATTTTGCTACTCCTTATCATTCTTGGGAGAGGGGATTAAACG
AGCATACTAATGGACTATTAAGACAATTTTTTCCCAAGGGAACGAATTTTAAAATTGTTAAGCCAGAGCAGGTGGAAATAGCCGTAAACTTGATTAACAA
TCGTCCTCGAAAATGTCTTGACTATCGTACCCCGAATGAGGTATTCTATAAAGGTAGATCAGACAGTGATGCAATTCAGACTTGAATTGGCC
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
987 bp328 aa991085+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSHQHLNIEQRNLLYQLSQEGNLSQRQMAVWLGCHQSTISRELKRNQSSLGCYLPDTAQAQSETRRKNAKQPFKNVSESALELVKEGLKDYHSPEQIAGR
LKRASQESLSHETIYQMIYQNYHGCGEYQKYLRRGLCQRKSRGGAKSKRGGIPGRVDISERPVIADQKTEIGHWESDTMIGGNHLGVLVTHVDKASKFLV
AGLAKNKTASEINRVTEKLFQEIHPDKRKTFTCDNGKEFCGHQELSQKLGADFYFATPYHSWERGLNEHTNGLLRQFFPKGTNFKIVKPEQVEIAVNLIN
NRPRKCLDYRTPNEVFYKGRSDSDAIQT

 

Blast result :
Comments
ISMae39 is 57% aa similar to ISPlu1.
References
1] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256