ISfinder

ISMae26

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010296	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1030 bp

Ends

IR Length : 25/32

IRL : GCAATTCTTGGAGCTATGAGGTACAATGGAAAGCAAATAGACAAATAATA
IRR : GCAATTCTCTGACTTATGAGGTACAGTAGCAACAGTGAGTAAACCAATTG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TCTCTTGACT	TATA	AATCCATCTG	4
GTGGCTAAGA	TATA	TATTGATTGC	4
CTTGGATATT	TATA	TCATAGTGAG	4
ATGAACTGAA	TATA	ATCTAGATAG	4
GAAATTGATT	TATA	GTTTCCAACT	4
TAAAGCCATA	TATA	ATACTAATGG	4
CATTCCTGAA	TATA	AGTTTATTAA	4
AGATGGAGAA	TATA	TTTAGAAGTT	4
ATAATCTGAA	TATA	AACTAATCAA	4
TTTTTCTACT	TATA	GATGAGGAAA	4
CTATAACAAT	TATA	TCAGCTTTGA	4

DNA sequence

GCAATTCTTGGAGCTATGAGGTACAATGGAAAGCAAATAGACAAATAATAGAACTTACCGGCTTCCTATGAGACCCTATTCCGTAGATTTTCGCCAAAAA
ATTATCGATGTCTGGAAGAAAGAAAAAATTTCCATTCGAGGACTAGCCCAGAGATTTGACGTGGCTAAAAGCTTTATTCAGAAGTTATTGAAGCAACATA
AAGAAACAGGAGATATCCGTCCTCGTCCGCAAGGGGGAAGTCCGCCAACCAAGTTAAATAGTGAACAACTGATAATTTTAATAGAAATCATCGAAGCTAA
CAATGATGCAACTCTCGAAGAATTATCGGACTTACTGTATGAAAAGACACAGGTGAAAGTCAGTAGAGCCACCCTGGGGCGGCTTACGCAAAAACTCAAT
TACAGTTTCAAAAAAAAACACTACACGCGGCGGAAAAAGAAAGTGACAGGGTACAGCAAAAAAGAGTGGAATACTGGTCAGAAGTTCGGGAAATTGAGGC
ATCAAAACTAATTTTCATCGATGAATCGGGGGTAAATTTAGCCCTTTTGAGACTCTATGCTAGAGCCTTAATTGGCCGAAGAGCTCGGGGAAGAAAACCA
CAAAAAAGAGGGAGAAATATTTCAATAATTAGTGCTATAAGCTTAGAAAAAGTTGTCGCATCAGTCAACATATACGGTGCAGTGGATGCGGTAACATTTG
AAGGATTTATTCTAAAGGAAGTGCTGCCAAAAATTAAAGAAGGAGACTGTCTGATCATGGATAACGCCAAGATTCATCTCGGAGAAATGGTCAGAGAAAT
AATCGAACAAGAAAAAGCTAGACTCATCTATTTACCTCCTTATTCTCCCGAATTCTCTCCTATTGAAAACTTTTGGTCAAAAGTGAAAGCGACGTTAAGA
AAACTGAAGGCGAGAACTTACAAAGACTTAATAGAAGGGATTGAATTGGCTATGTTAGAAGTTACTCAAAAAGATATTCGCAATTGGTTTACTCACTGTT
GCTACTGTACCTCATAAGTCAGAGAATTGC

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
444 bp	147 aa	68	511	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MRPYSVDFRQKIIDVWKKEKISIRGLAQRFDVAKSFIQKLLKQHKETGDIRPRPQGGSPPTKLNSEQLIILIEIIEANNDATLEELSDLLYEKTQVKVSR
ATLGRLTQKLNYSFKKKHYTRRKKKVTGYSKKEWNTGQKFGKLRHQN

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
651 bp	216 aa	367	1017	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

SHPGAAYAKTQLQFQKKTLHAAEKESDRVQQKRVEYWSEVREIEASKLIFIDESGVNLALLRLYARALIGRRARGRKPQKRGRNISIISAISLEKVVASV
NIYGAVDAVTFEGFILKEVLPKIKEGDCLIMDNAKIHLGEMVREIIEQEKARLIYLPPYSPEFSPIENFWSKVKATLRKLKARTYKDLIEGIELAMLEVT
QKDIRNWFTHCCYCTS

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
950 bp	316 aa	68	1017	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRPYSVDFRQKIIDVWKKEKISIRGLAQRFDVAKSFIQKLLKQHKETGDIRPRPQGGSPPTKLNSEQLIILIEIIEANNDATLEELSDLLYEKTQVKVSR
ATLGRLTQKLNYSFKKKTLHAAEKESDRVQQKRVEYWSEVREIEASKLIFIDESGVNLALLRLYARALIGRRARGRKPQKRGRNISIISAISLEKVVASV
NIYGAVDAVTFEGFILKEVLPKIKEGDCLIMDNAKIHLGEMVREIIEQEKARLIYLPPYSPEFSPIENFWSKVKATLRKLKARTYKDLIEGIELAMLEVT
QKDIRNWFTHCCYCTS

Blast result :

Comments

ISMae26 is 55% aa similar to ISSoc15.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.

References

1] Modified and submitted by ISfinder (2010)

2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.