ISfinder

ISMae23

Family IS630
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_010296	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1230 bp

Ends

IR Length : 13/17

IRL : AGCTAAAACGCACTTAAACCACCTAAAATAGAAATAGTAATTTTAGAAAA
IRR : AGCTTTTACGCATTTAAGTTACATTGACAGCATTACCCTTATTTTTAAGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AATTGATGGT	TA	TCGGGGACAA	2
AAATTCCAGA	TA	CCATCATGTC	2
AAATTCCAGA	TA	CCATCATGTC	2
CCAACATACA	TA	AAAAGATATG	2

DNA sequence

AGCTAAAACGCACTTAAACCACCTAAAATAGAAATAGTAATTTTAGAAAAATCCTACTTATGCCAGCAAAAGATTTCCTAGACTTAGAAGAAAAGAAAAA
CTTACAAAAAGCTCTTAAAGAAGAAGAAAGAGCAGAGGTTAGGGAAAGAATTTTAATGTTTCTCTTGCTAAACGACGGAAAAACTCAACGAGAAATAGCT
GACTTTATTGGCTGTTCACTCAAAACGGTCGCCCCTTGGTGTGTTCACGGTGATCCTAACAATTTAGAAAGTCTAGAAGATGGGAGAAAAAATGGAAATC
ATAAAAAAGCCACAGAGGAATATATTAATTTACTATTAAAAATAGTTGATGAAGACCCGAAGGAATTTGGATATGAATTCGGGAGATGGACAGCGGCAAG
ATTAGCAGAGCATCTAGAAAAGGAAACAAGAATTAAACTGAGTGGCTCGCAAGTGAGAAGAATATTGAGAAGAAAAAAGTATGTGTATATCTGGGCGAAA
TATAGTCTAGAAGATAAGCAAGACAAGAAATTAAGAAAAGCATTTAAAGAAAAATTAGATGAATATTTAAGGTTGGCTAAAGAAAAGCCAGAGTCAATCC
AGGTATGGTTTTGGGACGGGGCTTTCAAGGTGGCGACGCAGGTTCGCCACACAGCCCCTCATGAGTGTGGATTTAGTTTGAGAGTAATAAGAAGGAGAGC
TTGGACAAAAAAAGGAAAGCGAAAAAAAGTGAATGGACAAAGAAAAAGAGGAAGGGTGAATGTGATGGGAGCCTTAAGATATAATGACAAAAAACGAGTC
TGTTTTATGATCAAAAAAGGAAATTCAGAAACTTTCCATGAACAATTAAAGAAACTTCATGAAGAGATTCGTCAAGAATGGATAAACTTGGGAAATCTGC
CCGAAGATTTTCGAGAAAAAGGACCGAAAATTATCATCATATTAGACAATGCTAGTTATCACAAAAAGAAAGATGTTATTGAGCAGGTGGAAAAAGAGTT
GCCCAATATTAGGCTAGAGTTTTTACCAGCTTATAGTCCAGATTACAACCTAATAGAATTAGTGTGGCATTCCGCTAAAGAGTACATAGCTAATCGAGAA
TTTGAAAATAAAGAAGAACTGGAAAAAGTAGTCAATCAGCTTTTAAATGAAGGGGGATTGATTATTAAATGGAGTAGAAAACTTAAAAATAAGGGTAATG
CTGTCAATGTAACTTAAATGCGTAAAAGCT

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1158 bp	385 aa	60	1217	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MPAKDFLDLEEKKNLQKALKEEERAEVRERILMFLLLNDGKTQREIADFIGCSLKTVAPWCVHGDPNNLESLEDGRKNGNHKKATEEYINLLLKIVDEDP
KEFGYEFGRWTAARLAEHLEKETRIKLSGSQVRRILRRKKYVYIWAKYSLEDKQDKKLRKAFKEKLDEYLRLAKEKPESIQVWFWDGAFKVATQVRHTAP
HECGFSLRVIRRRAWTKKGKRKKVNGQRKRGRVNVMGALRYNDKKRVCFMIKKGNSETFHEQLKKLHEEIRQEWINLGNLPEDFREKGPKIIIILDNASY
HKKKDVIEQVEKELPNIRLEFLPAYSPDYNLIELVWHSAKEYIANREFENKEELEKVVNQLLNEGGLIIKWSRKLKNKGNAVNVT

Blast result :

Comments

ISMae23 is 78% aa similar to ISNpu2.

References

1] Modified and submitted by ISfinder (2010)

2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256.