ISMra5
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010518 | ND | Methylobacterium radiotolerans | Methylobacterium radiotolerans JCM 2831 plasmid pMRAD01 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD05 Methylobacterium radiotolerans JCM 2831 plasmid pMRAD04 |
DNA section
IS Length : 832 bp
Ends
IR Length : 15/17
IRL : GCGTCCGGACCCATAACGGTGGCGCGGACGGCTTGGGTGTGATTCACGGC
IRR : CCGTCCGGACTCATAACCAGTAGCTGACGGTGGCGGCGAGGCAGACGGCG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GCGGCTCTTC | TA | AGAGGTAGCC | 2 |
GCTCGGTCGT | TA | GTTGCGGACG | 2 |
AAGCCTAAGC | TA | AAGCACCCCT | 2 |
DNA sequence
GCGTCCGGACCCATAACGGTGGCGCGGACGGCTTGGGTGTGATTCACGGCTCTTGCGGGAGGGGCCGATGGACCATTTCTGGCTGACGGACGAGCAGTTT
GCGAGGATCGCACCGCTTCTGCCAAACGACACACGGGGCAAGGAGCGCGTGGACGACCGGCGCGTGATCAGCGGCATCGTCCACGTGCTGAAATCTGGAG
GGCGCTGGACGGACGCGCCACGCGATCTCTACGGGCCGAAGAAGACCCTCTACAATCGCTTCGTGCGCTGGGCGGCCAAGGGGGTCTGGATCGGGCTGTT
CGAGACCCTCGCTCAAGCCGGTGGGCCGCCCTCTCAAGTGCTGATCGACTCCACCGCCGTGAAGGCCCACCGCTGCGCCGCAGGAGGCAAGGGGGGGAGC
AGAGCCAAGCCATCGGTCGCTCGCGGGGCGGGCGCACGACCAAGATCCACGCCCTCACCGACCGCTTCTGCCGTCCGCTCGCGTTCCTGCTGACCGGCGG
GCAGGCTGCCGACTGCAAGGCTGGCGCGCTGCTGCTCGAACGCTTGCCCTCCTGCCGCATCGTGCTGGCGGACAAGGGCTACGACAGCGACGCCATTCGC
CGGCAGATTGAGGCGGCCGGGGCCGCACCCAACATCCCGCCCAAGATCAACCGCCGCTGGAAGCCGTGCTTCTCCCCGGTGCTCTATCGCGGGCGCAACG
CCATCGAGCGCATGTTCGGCCGCCTCAAGGACTTCCGCCGCATCGCCACCCGCTACGACCGTCTGGCGACCAACTATCTCGCCGCCGTCTGCCTCGCCGC
CACCGTCAGCTACTGGTTATGAGTCCGGACGG
GCGAGGATCGCACCGCTTCTGCCAAACGACACACGGGGCAAGGAGCGCGTGGACGACCGGCGCGTGATCAGCGGCATCGTCCACGTGCTGAAATCTGGAG
GGCGCTGGACGGACGCGCCACGCGATCTCTACGGGCCGAAGAAGACCCTCTACAATCGCTTCGTGCGCTGGGCGGCCAAGGGGGTCTGGATCGGGCTGTT
CGAGACCCTCGCTCAAGCCGGTGGGCCGCCCTCTCAAGTGCTGATCGACTCCACCGCCGTGAAGGCCCACCGCTGCGCCGCAGGAGGCAAGGGGGGGAGC
AGAGCCAAGCCATCGGTCGCTCGCGGGGCGGGCGCACGACCAAGATCCACGCCCTCACCGACCGCTTCTGCCGTCCGCTCGCGTTCCTGCTGACCGGCGG
GCAGGCTGCCGACTGCAAGGCTGGCGCGCTGCTGCTCGAACGCTTGCCCTCCTGCCGCATCGTGCTGGCGGACAAGGGCTACGACAGCGACGCCATTCGC
CGGCAGATTGAGGCGGCCGGGGCCGCACCCAACATCCCGCCCAAGATCAACCGCCGCTGGAAGCCGTGCTTCTCCCCGGTGCTCTATCGCGGGCGCAACG
CCATCGAGCGCATGTTCGGCCGCCTCAAGGACTTCCGCCGCATCGCCACCCGCTACGACCGTCTGGCGACCAACTATCTCGCCGCCGTCTGCCTCGCCGC
CACCGTCAGCTACTGGTTATGAGTCCGGACGG
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
426 bp | 141 aa | 68 | 493 | + | No |
Description : First part of the transposase
ORF sequence :
MDHFWLTDEQFARIAPLLPNDTRGKERVDDRRVISGIVHVLKSGGRWTDAPRDLYGPKKTLYNRFVRWAAKGVWIGLFETLAQAGGPPSQVLIDSTAVKA
HRCAAGGKGGSRAKPSVARGAGARPRSTPSPTASAVRSRSC
HRCAAGGKGGSRAKPSVARGAGARPRSTPSPTASAVRSRSC
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
582 bp | 193 aa | 241 | 822 | + | No |
Description : Second part of the transposase
ORF sequence :
EDPLQSLRALGGQGGLDRAVRDPRSSRWAALSSADRLHRREGPPLRRRRQGGEQSQAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQAADCKAGALLLE
RLPSCRIVLADKGYDSDAIRRQIEAAGAAPNIPPKINRRWKPCFSPVLYRGRNAIERMFGRLKDFRRIATRYDRLATNYLAAVCLAATVSYWL
RLPSCRIVLADKGYDSDAIRRQIEAAGAAPNIPPKINRRWKPCFSPVLYRGRNAIERMFGRLKDFRRIATRYDRLATNYLAAVCLAATVSYWL
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
755 bp | 251 aa | 68 | 822 | + | Yes |
Chemistry : DDE
ORF sequence :
MDHFWLTDEQFARIAPLLPNDTRGKERVDDRRVISGIVHVLKSGGRWTDAPRDLYGPKKTLYNRFVRWAAKGVWIGLFETLAQAGGPPSQVLIDSTAVKA
HRCAAGGKGGEQSQAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQAADCKAGALLLERLPSCRIVLADKGYDSDAIRRQIEAAGAAPNIPPKINRRWKP
CFSPVLYRGRNAIERMFGRLKDFRRIATRYDRLATNYLAAVCLAATVSYWL
HRCAAGGKGGEQSQAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQAADCKAGALLLERLPSCRIVLADKGYDSDAIRRQIEAAGAAPNIPPKINRRWKP
CFSPVLYRGRNAIERMFGRLKDFRRIATRYDRLATNYLAAVCLAATVSYWL
Blast result :
Comments
ISMra5 is 94% aa similar to ISMex15.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C.J. and Richardson,P. (2008) Direct submission Genbank.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C.J. and Richardson,P. (2008) Direct submission Genbank.