ISMra2
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_010505 | ND | Methylobacterium radiotolerans | Methylobacterium radiotolerans JCM 2831 plasmid pMRAD01 Methylobacterium radiotolerans JCM 2831 |
DNA section
IS Length : 905 bp
Ends
IR Length : 14
IRL : GGCGTCTCTGCAAAGGGTTCACAGGCCGCGGGATCGGCCCCATGTGGGCC
IRR : GGCGTCTCTGCAAACTCATGTGAGCCAGAGCATGGTCATGCCGAGGGTGA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GCTCGGTCCG | CTAG | TAGACCTGAG | 4 |
ATGTTGGCGC | CTAG | CCGGATCTTC | 4 |
ACGATCTCGA | CTAG | TCTGTCGAGT | 4 |
DNA sequence
GGCGTCTCTGCAAAGGGTTCACAGGCCGCGGGATCGGCCCCATGTGGGCCTGATGCGACGCTTTGAACTGACTGACGCGCAGTGGGAGCAGATCGCCCCG
CTGCTGCCTCCGCAGAAGCCGCGCACGGGCCGGCCCGCCGAGGATCACCGCCAAGTGCTCAACGGCATGCTCTGGATCCTGCGTACCGGTGCTCCCTGGG
AGGACCTGCCGGCCCGCTACGGGGCGGTCGGAACCGTGTCGAGCCGGTTCTACCGGTGGCGCAAGGCGGGCGTGTTCGACCGGGTGCTGCAGCGCCTGCA
GGCGCAGGCGGACGCCCGCGGCGCCCTGGACTGGGATCTGCACTTCGTGGACGCCACGGTGGTGCGCGCCCACCAGCACGCCGCCGGGGCACGCCGGTCC
GGCGCCATCGGGGGGTGAGGCGACCGTCGAGGGCGTGGGCGAAGCGCTCGGGCGCAGCCAGGGCGGGTTCTCCACCAAGCTGCATCTGCGCGCCGAGGGC
GGCGGCAAGCCGATCGCGGCGGTGCTGACGGCCGGCGAAAGGCACGAGCAGTTCGCACTGGACGCCTTGATGGACAAGGGCGCGGTGCCACGTCCAGGGC
GAGGCCGCCCGCGCCTGCGGCCCCGTAGGACGGCAGGGGACCGGGGTTACTCCAGTCCGCCGGCGCGTTGTCGCCTCAGGCAGCGTCGGATCGAGCCGGT
CATCCCGACCCGCAAGGACCAGCCGCGTCAGCCCGACTTTGACAAGGCCGCGTACCGCGAGCGCAACAAGGTCGAGCGGCTGATCAACCGCCTCAAGCAG
TATCGCCGCATCGCCACCCGCTACGAGAAGCGGGCTGCCAACTACCTCGCCATGGTCACCCTCGGCATGACCATGCTCTGGCTCACATGAGTTTGCAGAG
ACGCC
CTGCTGCCTCCGCAGAAGCCGCGCACGGGCCGGCCCGCCGAGGATCACCGCCAAGTGCTCAACGGCATGCTCTGGATCCTGCGTACCGGTGCTCCCTGGG
AGGACCTGCCGGCCCGCTACGGGGCGGTCGGAACCGTGTCGAGCCGGTTCTACCGGTGGCGCAAGGCGGGCGTGTTCGACCGGGTGCTGCAGCGCCTGCA
GGCGCAGGCGGACGCCCGCGGCGCCCTGGACTGGGATCTGCACTTCGTGGACGCCACGGTGGTGCGCGCCCACCAGCACGCCGCCGGGGCACGCCGGTCC
GGCGCCATCGGGGGGTGAGGCGACCGTCGAGGGCGTGGGCGAAGCGCTCGGGCGCAGCCAGGGCGGGTTCTCCACCAAGCTGCATCTGCGCGCCGAGGGC
GGCGGCAAGCCGATCGCGGCGGTGCTGACGGCCGGCGAAAGGCACGAGCAGTTCGCACTGGACGCCTTGATGGACAAGGGCGCGGTGCCACGTCCAGGGC
GAGGCCGCCCGCGCCTGCGGCCCCGTAGGACGGCAGGGGACCGGGGTTACTCCAGTCCGCCGGCGCGTTGTCGCCTCAGGCAGCGTCGGATCGAGCCGGT
CATCCCGACCCGCAAGGACCAGCCGCGTCAGCCCGACTTTGACAAGGCCGCGTACCGCGAGCGCAACAAGGTCGAGCGGCTGATCAACCGCCTCAAGCAG
TATCGCCGCATCGCCACCCGCTACGAGAAGCGGGCTGCCAACTACCTCGCCATGGTCACCCTCGGCATGACCATGCTCTGGCTCACATGAGTTTGCAGAG
ACGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
366 bp | 121 aa | 53 | 418 | + | No |
Description : First part of the transposase
ORF sequence :
MRRFELTDAQWEQIAPLLPPQKPRTGRPAEDHRQVLNGMLWILRTGAPWEDLPARYGAVGTVSSRFYRWRKAGVFDRVLQRLQAQADARGALDWDLHFVD
ATVVRAHQHAAGARRSGAIGG
ATVVRAHQHAAGARRSGAIGG
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
588 bp | 195 aa | 303 | 890 | + | No |
Description : Second part of the transposase
ORF sequence :
RRRTPAAPWTGICTSWTPRWCAPTSTPPGHAGPAPSGGEATVEGVGEALGRSQGGFSTKLHLRAEGGGKPIAAVLTAGERHEQFALDALMDKGAVPRPGR
GRPRLRPRRTAGDRGYSSPPARCRLRQRRIEPVIPTRKDQPRQPDFDKAAYRERNKVERLINRLKQYRRIATRYEKRAANYLAMVTLGMTMLWLT
GRPRLRPRRTAGDRGYSSPPARCRLRQRRIEPVIPTRKDQPRQPDFDKAAYRERNKVERLINRLKQYRRIATRYEKRAANYLAMVTLGMTMLWLT
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
838 bp | 278 aa | 53 | 890 | + | Yes |
Chemistry : DDE
ORF sequence :
MRRFELTDAQWEQIAPLLPPQKPRTGRPAEDHRQVLNGMLWILRTGAPWEDLPARYGAVGTVSSRFYRWRKAGVFDRVLQRLQAQADARGALDWDLHFVD
ATVVRAHQHAAGARRSGAIGGEATVEGVGEALGRSQGGFSTKLHLRAEGGGKPIAAVLTAGERHEQFALDALMDKGAVPRPGRGRPRLRPRRTAGDRGYS
SPPARCRLRQRRIEPVIPTRKDQPRQPDFDKAAYRERNKVERLINRLKQYRRIATRYEKRAANYLAMVTLGMTMLWLT
ATVVRAHQHAAGARRSGAIGGEATVEGVGEALGRSQGGFSTKLHLRAEGGGKPIAAVLTAGERHEQFALDALMDKGAVPRPGRGRPRLRPRRTAGDRGYS
SPPARCRLRQRRIEPVIPTRKDQPRQPDFDKAAYRERNKVERLINRLKQYRRIATRYEKRAANYLAMVTLGMTMLWLT
Blast result :
Comments
ISMra2 is 61% aa similar to IS1650.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible +1 frameshift.
References
1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C.J. and Richardson,P. (2008) Direct submission Genbank.
2] Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kiss,H., Brettin,T., Detter,J.C., Han,C., Kuske,C.R., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N., Marx,C.J. and Richardson,P. (2008) Direct submission Genbank.