ISMno31
- Family IS3
- Group IS150
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_011894 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 |
DNA section
IS Length : 1510 bp
Ends
IR Length : 23/33
IRL : TGTCCGCCGTCAGCGCCCGTGAGACAGCTTGGGGCCTTTCACGACCGGAG
IRR : TGTCCGTCGTCAGATGATTTGAGACGGTTTGCGACGCTCAGGAGCGGAGG
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GGTATACCCC | AACGAGAGGA | 0 |
DNA sequence
TGTCCGCCGTCAGCGCCCGTGAGACAGCTTGGGGCCTTTCACGACCGGAGGATGGCTGGTTCATCGTAGCCCCCAGGAGCGAAGATGAAGCAGACATCCG
GGCCGGCGCGGAAGCCGGCAGAGGCGGTGATCAAGGACATCCGCCGCGCCACGCGCCGGCCGTTCTCGGCCGAGGAGAAGATCCGCATCGTGCTGGAGGG
CCTGCGCGGCGAGGACAGCATCGCCGAGCTGTGCCGGCGTGAAGGCATCGCCACCTCGATGTACTACGGCTGGTCCAAGGAGTTTCTGGAGGCTGGCAAG
CGGCGGCTGGCCGGCGACACGGCCCGCGCTGCCACCGCCGATGAGGTCAAGGACCTGCGGCGCGAGGCCAGCGCGCTCAAGGAGGTTGTGGCCGATCTCA
CGCTGGAGAACCGCCTGCTCAAAAAAAGCATGAGCGGGGCTGGGGAGGACGGGGCATGAGGTACCCGGCCCCTGAGAAGCTGGAGATCATCCGGCTGGTC
GAGCAGTCCCACCTGCCGGTGCGCCGCACCCTGGAGAAGCTCGGCATCCCGCGCGCCACCTTCTACCGCTGGTACGACGCCTACCAGCGCGGTGGACCCG
AGGCCCTGGCCGACCGACCCTCTCGGCCCAGCCGGGTCTGGAACCGTCTGCCCGAGGAGGTCCGCGAGCAGATCGTCGCCCTCGCGCTGGAGCAGCCGGA
ACTCAGCCCACGCGAGCTGGCGGTGCGCTTCACCGACGAGCGGCAGTACTTCGTCTCGGAGGCGACGGTCTACCGTCTGCTCAAGGCACAGGACCTGATC
ACCAGCCCGGCCTACATCGTCGTCACGGCGGCCGACGAGTTCCGCGACACGACCACGGCCCCCAACCAGCTCTGGCAGACGGACTTCACCTCCCTGAAGG
TGGTCGGCTGGGGCTGGTACTACCTGTCGACGGTGCTCGACGACTACTCACGCTTCATCGTGGCCTGGAAGCTCTGCGCCACGATGCAGGCCAGCGACGT
CACCGCCACGCTCGACCTGGCGCTGAGCGCCGCCGGGCTCGATCAGGTGCAGGTCGTGCATCGGCCGCGGCTGCTGACCGACAACGGATCGAGCTACGTC
GCCGGTGACTTGGCCCCATGGCTCGGCAGTCGCGGCATGCCCCACATCCGCGGGGCCCCGCGCCATCCTCAGACCCAGGGCAAGATCGAGCGCTGGCATC
AGACGCTCAAGAACCGCATTCTGCTCGAACACGCCTACCTGCCCAGCGAACTGGAGGCGCGGGTCGCTGCCTTCGTCGAGCACTACAATCACGTCCGGGC
TCACGAAAGCCTCGGCAACCTCACGCCGGCCGACGTCTACTTCGGCCGCGGCGAGGCGATCCTGCGCGAGCGGGCGCGCATCAAACGCCAGACCCTCACC
GATCGCCGCTTGCGCCACCACGCGCAGGCTGCCTAACTTCTCAACCCAGATGGACCAGAGCCTCCGCTCCTGAGCGTCGCAAACCGTCTCAAATCATCTG
ACGACGGACA
GGCCGGCGCGGAAGCCGGCAGAGGCGGTGATCAAGGACATCCGCCGCGCCACGCGCCGGCCGTTCTCGGCCGAGGAGAAGATCCGCATCGTGCTGGAGGG
CCTGCGCGGCGAGGACAGCATCGCCGAGCTGTGCCGGCGTGAAGGCATCGCCACCTCGATGTACTACGGCTGGTCCAAGGAGTTTCTGGAGGCTGGCAAG
CGGCGGCTGGCCGGCGACACGGCCCGCGCTGCCACCGCCGATGAGGTCAAGGACCTGCGGCGCGAGGCCAGCGCGCTCAAGGAGGTTGTGGCCGATCTCA
CGCTGGAGAACCGCCTGCTCAAAAAAAGCATGAGCGGGGCTGGGGAGGACGGGGCATGAGGTACCCGGCCCCTGAGAAGCTGGAGATCATCCGGCTGGTC
GAGCAGTCCCACCTGCCGGTGCGCCGCACCCTGGAGAAGCTCGGCATCCCGCGCGCCACCTTCTACCGCTGGTACGACGCCTACCAGCGCGGTGGACCCG
AGGCCCTGGCCGACCGACCCTCTCGGCCCAGCCGGGTCTGGAACCGTCTGCCCGAGGAGGTCCGCGAGCAGATCGTCGCCCTCGCGCTGGAGCAGCCGGA
ACTCAGCCCACGCGAGCTGGCGGTGCGCTTCACCGACGAGCGGCAGTACTTCGTCTCGGAGGCGACGGTCTACCGTCTGCTCAAGGCACAGGACCTGATC
ACCAGCCCGGCCTACATCGTCGTCACGGCGGCCGACGAGTTCCGCGACACGACCACGGCCCCCAACCAGCTCTGGCAGACGGACTTCACCTCCCTGAAGG
TGGTCGGCTGGGGCTGGTACTACCTGTCGACGGTGCTCGACGACTACTCACGCTTCATCGTGGCCTGGAAGCTCTGCGCCACGATGCAGGCCAGCGACGT
CACCGCCACGCTCGACCTGGCGCTGAGCGCCGCCGGGCTCGATCAGGTGCAGGTCGTGCATCGGCCGCGGCTGCTGACCGACAACGGATCGAGCTACGTC
GCCGGTGACTTGGCCCCATGGCTCGGCAGTCGCGGCATGCCCCACATCCGCGGGGCCCCGCGCCATCCTCAGACCCAGGGCAAGATCGAGCGCTGGCATC
AGACGCTCAAGAACCGCATTCTGCTCGAACACGCCTACCTGCCCAGCGAACTGGAGGCGCGGGTCGCTGCCTTCGTCGAGCACTACAATCACGTCCGGGC
TCACGAAAGCCTCGGCAACCTCACGCCGGCCGACGTCTACTTCGGCCGCGGCGAGGCGATCCTGCGCGAGCGGGCGCGCATCAAACGCCAGACCCTCACC
GATCGCCGCTTGCGCCACCACGCGCAGGCTGCCTAACTTCTCAACCCAGATGGACCAGAGCCTCCGCTCCTGAGCGTCGCAAACCGTCTCAAATCATCTG
ACGACGGACA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
375 bp | 124 aa | 85 | 459 | + | No |
Description : First part of the transposase
ORF sequence :
MKQTSGPARKPAEAVIKDIRRATRRPFSAEEKIRIVLEGLRGEDSIAELCRREGIATSMYYGWSKEFLEAGKRRLAGDTARAATADEVKDLRREASALKE
VVADLTLENRLLKKSMSGAGEDGA
VVADLTLENRLLKKSMSGAGEDGA
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1092 bp | 363 aa | 345 | 1436 | + | No |
Description : Second part of the transposase
ORF sequence :
GQGPAARGQRAQGGCGRSHAGEPPAQKKHERGWGGRGMRYPAPEKLEIIRLVEQSHLPVRRTLEKLGIPRATFYRWYDAYQRGGPEALADRPSRPSRVWN
RLPEEVREQIVALALEQPELSPRELAVRFTDERQYFVSEATVYRLLKAQDLITSPAYIVVTAADEFRDTTTAPNQLWQTDFTSLKVVGWGWYYLSTVLDD
YSRFIVAWKLCATMQASDVTATLDLALSAAGLDQVQVVHRPRLLTDNGSSYVAGDLAPWLGSRGMPHIRGAPRHPQTQGKIERWHQTLKNRILLEHAYLP
SELEARVAAFVEHYNHVRAHESLGNLTPADVYFGRGEAILRERARIKRQTLTDRRLRHHAQAA
RLPEEVREQIVALALEQPELSPRELAVRFTDERQYFVSEATVYRLLKAQDLITSPAYIVVTAADEFRDTTTAPNQLWQTDFTSLKVVGWGWYYLSTVLDD
YSRFIVAWKLCATMQASDVTATLDLALSAAGLDQVQVVHRPRLLTDNGSSYVAGDLAPWLGSRGMPHIRGAPRHPQTQGKIERWHQTLKNRILLEHAYLP
SELEARVAAFVEHYNHVRAHESLGNLTPADVYFGRGEAILRERARIKRQTLTDRRLRHHAQAA
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1352 bp | 450 aa | 85 | 1436 | + | Yes |
Chemistry : DDE
ORF sequence :
MKQTSGPARKPAEAVIKDIRRATRRPFSAEEKIRIVLEGLRGEDSIAELCRREGIATSMYYGWSKEFLEAGKRRLAGDTARAATADEVKDLRREASALKE
VVADLTLENRLLKKKHERGWGGRGMRYPAPEKLEIIRLVEQSHLPVRRTLEKLGIPRATFYRWYDAYQRGGPEALADRPSRPSRVWNRLPEEVREQIVAL
ALEQPELSPRELAVRFTDERQYFVSEATVYRLLKAQDLITSPAYIVVTAADEFRDTTTAPNQLWQTDFTSLKVVGWGWYYLSTVLDDYSRFIVAWKLCAT
MQASDVTATLDLALSAAGLDQVQVVHRPRLLTDNGSSYVAGDLAPWLGSRGMPHIRGAPRHPQTQGKIERWHQTLKNRILLEHAYLPSELEARVAAFVEH
YNHVRAHESLGNLTPADVYFGRGEAILRERARIKRQTLTDRRLRHHAQAA
VVADLTLENRLLKKKHERGWGGRGMRYPAPEKLEIIRLVEQSHLPVRRTLEKLGIPRATFYRWYDAYQRGGPEALADRPSRPSRVWNRLPEEVREQIVAL
ALEQPELSPRELAVRFTDERQYFVSEATVYRLLKAQDLITSPAYIVVTAADEFRDTTTAPNQLWQTDFTSLKVVGWGWYYLSTVLDDYSRFIVAWKLCAT
MQASDVTATLDLALSAAGLDQVQVVHRPRLLTDNGSSYVAGDLAPWLGSRGMPHIRGAPRHPQTQGKIERWHQTLKNRILLEHAYLPSELEARVAAFVEH
YNHVRAHESLGNLTPADVYFGRGEAILRERARIKRQTLTDRRLRHHAQAA
Blast result :
Comments
ISMno31 is 94% aa similar to ISMch3.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by a possible -1 frameshift.
References
1] Ming-Chun Lee and David Robinson (2009) Direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.