ISMno11
- Family IS630
- Group
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
NC_011894 | ND | Methylobacterium nodulans | Methylobacterium nodulans ORS 2060 Methylobacterium nodulans ORS 2060 plasmid pMNOD02 Methylobacterium nodulans ORS 2060 plasmid pMNOD01 |
DNA section
IS Length : 1058 bp
Ends
IR Length : 18/25
IRL : GAGCGGGGTCCGAGCGGGTTGAAACCGTGGTGATGGCAACGCGTTGGCCG
IRR : GAGCCGCTCCCGATCAGGTTGAAGCGTAAGCATCATCCTCGTATCCAGCA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GGCCAGGCTC | TA | GCACAGACCG | 2 |
GCTCGGTCTC | TA | GGTGCTCCTC | 2 |
GGGAGCGCTC | TA | GACCCGGCAA | 2 |
GGAGTTGGGC | TA | GGCCAGAGGT | 2 |
TCAGCGCCGC | TA | GCCGCGGCAG | 2 |
CCGCGATCAC | TA | GGCCGGCCAC | 2 |
AGCGGCGTCC | TA | GCTCGCGCCG | 2 |
GGATGGAAGC | TA | GCCCACGCTC | 2 |
CCTCCGAGGC | TA | AGGGCAGCAG | 2 |
TTCCGCGCTC | TA | GCGCAGCAGA | 2 |
CACAGATCAC | TA | GAGAGGATGT | 2 |
TTTTGACCAC | TA | GAGCATTTTC | 2 |
DNA sequence
GAGCGGGGTCCGAGCGGGTTGAAACCGTGGTGATGGCAACGCGTTGGCCGGTCCCCGGGGGGGCGGCTCAACGGAGGTGGCCCATGCCCTCAGCTTTGTC
TGTCGACCTGCGCCAGCGCGTCGTCTCGGCCGTTGCAGAAGGCGCCTCCTGCCATCAGGCCGCCGCGCGCTTCGGGGTCAGCGTGGCCAGCGTCAGCCGC
TGGTCCAGGCAGCAGGAGGGCCAGGGAGATGTCACGCCCAAGCCCATGGGCGGCGACCAGCGCTCGCAGCGCATCGAGGCTCATGCCGAGCTCATCCTGC
AGACCTACCAAGCCCACCCGCAGAGCTTCCTGCATGAGCTCTGCGAGACGCTCCAGGAGCAAGGCGTTCCGGTCAGCCGCAGTAGCCTGTCGCGCTTCTT
CGCCCGGCACCGCATCACGCGGAAAAAAGGGCGACGTACGCAGCTGAGCAGGAGCGGGAGGATGTAAAGGCGGCTCGTGAGGCGTGGTTTGCCGGCCAGC
TTGAGCTGGATCCGGAGCGACTGGTGTTCCTGGATGAGACCGCCGCCACCACCAGCATGGTCCGCCGCTACGGCTGGGCCCCGCGCGGCGAGCGTTGCCG
CCTCGCGGCACCCGCAGGGCACTGGAAAACCACCACTGTGATTGCCGGGCTGCGCACGAGCGGGCCTGACGCGATCGCTCTGCTGGACGGCCCTGTGACG
GGCGAACGCTTCCGCGCCTACGTGACCGACACCCTGGTCCCCACCCTGAGACCCGGCGACACCGTGATCCTGGACAATCTGGGCGCTCACAAGGTGGCCG
GCGTGCGCGAGGCGATCGCGGCAACCGGGGCCCGGCTGCTTTATCTTCCTCCGTACTCACCTGAGTTCAACCCGATTGAGCAGGCCTTCGCCAAGCTGAA
AGCGCTGTTGCGCAGGGCGGCGGCCCGCAGTGTCAGTGAACTCTGGGCGGCGATCCACCAAGCCTTCAAATGCTTCTCGCCGGCTGAGTGCCGCAACTAC
TTCACAGCTGCTGGATACGAGGATGATGCTTACGCTTCAACCTGATCGGGAGCGGCTC
TGTCGACCTGCGCCAGCGCGTCGTCTCGGCCGTTGCAGAAGGCGCCTCCTGCCATCAGGCCGCCGCGCGCTTCGGGGTCAGCGTGGCCAGCGTCAGCCGC
TGGTCCAGGCAGCAGGAGGGCCAGGGAGATGTCACGCCCAAGCCCATGGGCGGCGACCAGCGCTCGCAGCGCATCGAGGCTCATGCCGAGCTCATCCTGC
AGACCTACCAAGCCCACCCGCAGAGCTTCCTGCATGAGCTCTGCGAGACGCTCCAGGAGCAAGGCGTTCCGGTCAGCCGCAGTAGCCTGTCGCGCTTCTT
CGCCCGGCACCGCATCACGCGGAAAAAAGGGCGACGTACGCAGCTGAGCAGGAGCGGGAGGATGTAAAGGCGGCTCGTGAGGCGTGGTTTGCCGGCCAGC
TTGAGCTGGATCCGGAGCGACTGGTGTTCCTGGATGAGACCGCCGCCACCACCAGCATGGTCCGCCGCTACGGCTGGGCCCCGCGCGGCGAGCGTTGCCG
CCTCGCGGCACCCGCAGGGCACTGGAAAACCACCACTGTGATTGCCGGGCTGCGCACGAGCGGGCCTGACGCGATCGCTCTGCTGGACGGCCCTGTGACG
GGCGAACGCTTCCGCGCCTACGTGACCGACACCCTGGTCCCCACCCTGAGACCCGGCGACACCGTGATCCTGGACAATCTGGGCGCTCACAAGGTGGCCG
GCGTGCGCGAGGCGATCGCGGCAACCGGGGCCCGGCTGCTTTATCTTCCTCCGTACTCACCTGAGTTCAACCCGATTGAGCAGGCCTTCGCCAAGCTGAA
AGCGCTGTTGCGCAGGGCGGCGGCCCGCAGTGTCAGTGAACTCTGGGCGGCGATCCACCAAGCCTTCAAATGCTTCTCGCCGGCTGAGTGCCGCAACTAC
TTCACAGCTGCTGGATACGAGGATGATGCTTACGCTTCAACCTGATCGGGAGCGGCTC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
384 bp | 127 aa | 84 | 467 | + | No |
Description : First part of the transposase
ORF sequence :
MPSALSVDLRQRVVSAVAEGASCHQAAARFGVSVASVSRWSRQQEGQGDVTPKPMGGDQRSQRIEAHAELILQTYQAHPQSFLHELCETLQEQGVPVSRS
SLSRFFARHRITRKKGRRTQLSRSGRM
SLSRFFARHRITRKKGRRTQLSRSGRM
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
660 bp | 219 aa | 386 | 1045 | + | No |
Description : Second part of the transposase
ORF sequence :
PVALLRPAPHHAEKRATYAAEQEREDVKAAREAWFAGQLELDPERLVFLDETAATTSMVRRYGWAPRGERCRLAAPAGHWKTTTVIAGLRTSGPDAIALL
DGPVTGERFRAYVTDTLVPTLRPGDTVILDNLGAHKVAGVREAIAATGARLLYLPPYSPEFNPIEQAFAKLKALLRRAAARSVSELWAAIHQAFKCFSPA
ECRNYFTAAGYEDDAYAST
DGPVTGERFRAYVTDTLVPTLRPGDTVILDNLGAHKVAGVREAIAATGARLLYLPPYSPEFNPIEQAFAKLKALLRRAAARSVSELWAAIHQAFKCFSPA
ECRNYFTAAGYEDDAYAST
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
962 bp | 320 aa | 84 | 1045 | + | Yes |
Chemistry : DDE
ORF sequence :
MPSALSVDLRQRVVSAVAEGASCHQAAARFGVSVASVSRWSRQQEGQGDVTPKPMGGDQRSQRIEAHAELILQTYQAHPQSFLHELCETLQEQGVPVSRS
SLSRFFARHRITRKKRATYAAEQEREDVKAAREAWFAGQLELDPERLVFLDETAATTSMVRRYGWAPRGERCRLAAPAGHWKTTTVIAGLRTSGPDAIAL
LDGPVTGERFRAYVTDTLVPTLRPGDTVILDNLGAHKVAGVREAIAATGARLLYLPPYSPEFNPIEQAFAKLKALLRRAAARSVSELWAAIHQAFKCFSP
AECRNYFTAAGYEDDAYAST
SLSRFFARHRITRKKRATYAAEQEREDVKAAREAWFAGQLELDPERLVFLDETAATTSMVRRYGWAPRGERCRLAAPAGHWKTTTVIAGLRTSGPDAIAL
LDGPVTGERFRAYVTDTLVPTLRPGDTVILDNLGAHKVAGVREAIAATGARLLYLPPYSPEFNPIEQAFAKLKALLRRAAARSVSELWAAIHQAFKCFSP
AECRNYFTAAGYEDDAYAST
Blast result :
Comments
ISMno11 is 80% aa similar to ISMex30. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Ming-Chun Lee and David Robinson, direct submission.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.
2] Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E., Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Sims,D., Brettin,T., Detter,J.C., Han,C., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Ivanova,N., Marx,C.J. and Richardson,P. (2009) Direct submission GenBank.