ISMch8

  • Family IS5
  • Group IS5
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_011758 ND Methylobacterium chloromethanicum
Methylobacterium chloromethanicum CM4 plasmid pMCHL01
DNA section
IS Length : 1494 bp

Ends


IR Length : 17

IRL : CAGGCTGTTGAAGAAGTCCGTGGCATGGCAGAGCTGGGCATGATTCACTC
IRR : CAGGCTGTTGAAGAAGTGGGCCAGCGGCGAGCGATGAAGGGGATTTCGTC

Insertion site


Left flankDirect repeatRight flankDR Length
GCGCCTTGAGTTAGACGAGC0

DNA sequence

CAGGCTGTTGAAGAAGTCCGTGGCATGGCAGAGCTGGGCATGATTCACTCCTTCCCGGGAGCGCTTGGGGAAGGCGGATGCGAGGCTCGGATCAGCGGTC
GGGGACGTTGTTCTCCTATGTGGATCTGGAGGCGCGGGTCCGGCGCGACCACCCGCTGCGGACGATCCGGCAGCTCACGGATGTGGCGCTTGCAGCGCTG
AACCAGGACTTCTCTACGCTCTATGCGGCCCGAATGGGTCGTCCATCGATCCCGCCTGAGATGCTGCCGCGAGCGATACTCCTGCAGGCGTTCTACTCGA
TCCGCTCGGAACGGCAGTTGATGGAGCGGCTGGAATTCGACCTGTTGTTCCGCTGGTTCGTAGGCCTGGGTGTCGACGATCCGGCCTGGGATCATTCGAG
CTTCACCAAGAACCGGGACCGGCTGCTGGCCGGCGAGATTGCCGCCAAGTTCCTGGCCGCCGTGCTGGCCCAGCCCCGGGTGAAGCGGCTCCTGTCCAGC
GACCACTTCAGCGTCGACGGCACGCTGATCGAGGCCTGGGCGTCGATGAAGAGCTTCAGGCCCAAGGATCAAAGCGAGGACCAAGCACCTCCGCCCGCTG
GGAGGCGCAACGCCGAGGTCGACTTCAAGGGTCAGACACGCTCAAACGAGACGCACGAGAGCACCACCGATCCTGAAGCCAGGCTCTACCGCAAGGGGCG
AGGTAGGGAGGCCAAGCTCTGCTTCATCGGCCACGCCCTGATGGAGAACCGCTCGGCCCTGATCGTGGACACCCGCCTGACGTCTGCAGACGGCCACGCC
GAGCGCATCGCCGCCTTGGCGATGATCGAACCGAGGGCGGACCGGCCCAGGGCGATCTCCGTGGGCGCCGACAAGGCCTATGACGCCGAGGACTTCGTCA
ACGAGCTGGCATCGATGAACGTGCGAGCGCACGTGGCCCAGAACACCAACGGCCGCCGCTCCTCTATCGACAGACGAACCACCCGCCACGCCGGCTATGG
CACGAGCCAGAGGATCCGCAAGCGCATCGAGGAGGGCTTTGGCTGGATCAAGACCGTCGGCGGCCAGCGGAAAGCCCGCTTCAGAGGTCGCGACAAGGCC
GGGTGGGCCTTCGCCTTCACGGCGGCGGCCTACAACCTGGTTCGCCTGCCCAAGCTGCTTGGAGCTCTGGCGTGAGCGTCCGGGGCAAGTGGCGGATCGT
CGAGCTGCCCGGCTACGCGGACGACTACGCCGACATGGTCGAGCCGGCCTACATCCTCTTCGAGGCCGCCCGCGGCGAGTTCGCCTTCGGATGCGTCACC
GGGGCCTTCGCCAGCGGCGGCGATCACGCCGCCATTGCGTTCGACTGGACCGGCAACGATGAGATGGATGAGGCCCGCGGCTCGGGTTGGGCCGAGCTTC
AGGCCGACGGCTCGCTTCACGGCGGGATCGGCTTCCATGCCGGCGACGAAATCCCCTTCATCGCTCGCCGCTGGCCCACTTCTTCAACAGCCTG
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1098 bp365 aa781175+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MRGSDQRSGTLFSYVDLEARVRRDHPLRTIRQLTDVALAALNQDFSTLYAARMGRPSIPPEMLPRAILLQAFYSIRSERQLMERLEFDLLFRWFVGLGVD
DPAWDHSSFTKNRDRLLAGEIAAKFLAAVLAQPRVKRLLSSDHFSVDGTLIEAWASMKSFRPKDQSEDQAPPPAGRRNAEVDFKGQTRSNETHESTTDPE
ARLYRKGRGREAKLCFIGHALMENRSALIVDTRLTSADGHAERIAALAMIEPRADRPRAISVGADKAYDAEDFVNELASMNVRAHVAQNTNGRRSSIDRR
TTRHAGYGTSQRIRKRIEEGFGWIKTVGGQRKARFRGRDKAGWAFAFTAAAYNLVRLPKLLGALA

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
327 bp108 aa11721498+No
ORF function : Passenger Gene
Annotation : Hypothetical proteinDescription :

ORF sequence :

MSVRGKWRIVELPGYADDYADMVEPAYILFEAARGEFAFGCVTGAFASGGDHAAIAFDWTGNDEMDEARGSGWAELQADGSLHGGIGFHAGDEIPFIARR
WPTSSTAC

 

Blast result :
Comments
ISMch8 is 72% aa similar to ISGsu2.The first ORF is the transposase and the second is a passenger gene annotated as hypothetical protein.
References
1] Ming-Chun Lee and David Robinson, direct submission.