ISfinder

ISOih1

Family IS1182
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
BA000028	ND	Oceanobacillus iheyensis	Oceanobacillus iheyensis HTE831

DNA section

IS Length : 1563 bp

Ends

IR Length : 12/15

IRL : CAGGCTGTCGAGAAACCCTCGGCAGTCTATTTTTATGTGGGAAACAGCCC
IRR : CAGCTTGTAGAGAAAGTATGGTATTTTCTCTGCAAGCTTTTTTATATGGT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTTAGCACGTAA	AG/TAGCCTCCTTTTTGGGAGGCTCT	ATTTTTAGTG	24

DNA sequence

CAGGCTGTCGAGAAACCCTCGGCAGTCTATTTTTATGTGGGAAACAGCCCCAATTGACCGTAATTATTTGGTATAATAGAAGTACCAAACAAAGGCGGTG
ATGAGCTTGTTTCACACGAGAAAACACATGCAAAATGAAATGGAATTTGTTTGTTTAGAAGATTTAGTCCCCCAAGACCATTTACTACGGAAAATAGATA
AATACATTGATTTCTCTTTTATTATTGAAAAAGTAAAACCTTATTATTGTGAAGATAATGGTCGTCCCTCTCTAGACCCCCTTATTTTATTCAAAATGAT
GTTTGTTGGGTATTTTTTTGGCATTCGTTCGGAACGCCAACTTGAAAAGGAAATTCAAACAAATAATGCTTACCGCTGGTTTCTAGGTTTAAAACTGACA
GATCCAGTTCCTCACCATTCTACCATCAGTTGGAACCGTCGAACCCGTTTTAAAGATACGGAGATTTTCCAAGAAATTTTTGATGAAATTGTTCTCCAAG
CTATGAATCACCGTATGGTTGGAGGAAGAGTTCTTTTTACGGATTCCACTCATTTGAAAGCGAATGCCAATAAACATAAATTTAGTCGGGAAGAAGTCGA
AGTGGAAACACGAGACTACGTAGAAGAACTAAACCAAGCCATTCAAGAGGATCGTAAACAGAATGGAAAAAAAGGTCCTAAGAGATAGAGAGGAAGTGAA
ACAAGTCAAAACCATTCGGAAAAGTAAAACAGATCCGGAAAGTGGTTTTATGTCTCGGGACCACAAACAAGAAATGTTCTGTTACTTAGATCATCGAACC
ACAGATATGAAATATAATATTATTACAGACGCGTACGTCACTCCGGGAAATGTACATGATTCGGTCCCTTATCTGAAACGGTTAGACCGGCAAATCAAAC
GGTTTGATTTTATCGTGGAAGCAGTGGCTTTAGACTCCGGTTATTTAACGAATCCAATCTGTAAGGGATTAGCTGATCGGAATATATTCGGCGTCATCGC
TCACCGTCGCTATCATCCAACGAAAGGGTTATTTCCGAAGTGGAAATTCCGTTATGATGAAGACCGAGATAGTTATACTTGTCCGAATGGAGAAGAATTG
CCATATAAAACAACGACACGAGAAGGTTACAGAGAATTTAAATCAGACCCAAAAAAGTGTATATCCTGTCCGCTTTTGAAACAGTGTACCCGCTCGAATA
ATCATCAGAAAGTAGTCACCCGTCATGTTTGGGAAGAACATAAAGAACACGTGCGATTAAACCGGTTATCCGTTTCCGGAAAAAAGCTATACAAATTTAG
AAAAGAAAAAGTAGAGCGAAGTTTTGCAGACTCTAAAGAGCTGCATGGACTTCGCTACTGCAGGTTGAGGGGATTGCAGAATGCGGGAGAGCAAGTATTA
CTCACCGCAGCCTGCCAGAACATGAAGAAGATTGCCATCTACCTATCCAAGCAAGGATAGGTAGATGGGAGTTTATTTTCATGATATATCTTATTTTTCT
CTTGATTATTTTAACCATATAAAAAAGCTTGCAGAGAAAATACCATACTTTCTCTACAAGCTG

Recoding section

Recoding by frameshift
Frame
Type
Experimentally demonstrated

Stimulators :

Shine-Dalgarno sequence :
Secondary structure :

Recoding motif :

Protein section

ORF number : 3

ORF 1

Length		Begin	End	Strand	Fusion ORF
588 bp	195 aa	101	688	+	No

ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MSLFHTRKHMQNEMEFVCLEDLVPQDHLLRKIDKYIDFSFIIEKVKPYYCEDNGRPSLDPLILFKMMFVGYFFGIRSERQLEKEIQTNNAYRWFLGLKLT
DPVPHHSTISWNRRTRFKDTEIFQEIFDEIVLQAMNHRMVGGRVLFTDSTHLKANANKHKFSREEVEVETRDYVEELNQAIQEDRKQNGKKGPKR

Blast result :

ORF 2

Length		Begin	End	Strand	Fusion ORF
828 bp	275 aa	633	1460	+	No

ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

TKPFKRIVNRMEKKVLRDREEVKQVKTIRKSKTDPESGFMSRDHKQEMFCYLDHRTTDMKYNIITDAYVTPGNVHDSVPYLKRLDRQIKRFDFIVEAVAL
DSGYLTNPICKGLADRNIFGVIAHRRYHPTKGLFPKWKFRYDEDRDSYTCPNGEELPYKTTTREGYREFKSDPKKCISCPLLKQCTRSNNHQKVVTRHVW
EEHKEHVRLNRLSVSGKKLYKFRKEKVERSFADSKELHGLRYCRLRGLQNAGEQVLLTAACQNMKKIAIYLSKQG

Blast result :

ORF 3

Length		Begin	End	Strand	Fusion ORF
1360 bp	452 aa	101	1460	+	Yes

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MSLFHTRKHMQNEMEFVCLEDLVPQDHLLRKIDKYIDFSFIIEKVKPYYCEDNGRPSLDPLILFKMMFVGYFFGIRSERQLEKEIQTNNAYRWFLGLKLT
DPVPHHSTISWNRRTRFKDTEIFQEIFDEIVLQAMNHRMVGGRVLFTDSTHLKANANKHKFSREEVEVETRDYVEELNQAIQEDRKQNGKKVLRDREEVK
QVKTIRKSKTDPESGFMSRDHKQEMFCYLDHRTTDMKYNIITDAYVTPGNVHDSVPYLKRLDRQIKRFDFIVEAVALDSGYLTNPICKGLADRNIFGVIA
HRRYHPTKGLFPKWKFRYDEDRDSYTCPNGEELPYKTTTREGYREFKSDPKKCISCPLLKQCTRSNNHQKVVTRHVWEEHKEHVRLNRLSVSGKKLYKFR
KEKVERSFADSKELHGLRYCRLRGLQNAGEQVLLTAACQNMKKIAIYLSKQG

Blast result :

Comments

ISOih1 is 58% aa similar to ISSag8. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.

References

1] ISfinder annotation (2005)
2] Lu,J., Nogi,Y. and Takami,H.(2001) FEMS Microbiol. Lett. 205 (2), 291-297