ISfinder

ISMhu1

Family IS1182
Group

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_007796	ND	Methanospirillum hungatei	Methanospirillum hungatei JF-1

DNA section

IS Length : 1742 bp

Ends

IR Length : 15/17

IRL : GAGGGTGTTGCACAACTCAGATCCCCCCATAATTTTTTAACTAAGGTCAA
IRR : GAGGGTGTTGCATGACTAAAATTTGGAAAATTTTTGGTAAGTACTTTTTC

Insertion site

Left flank	Direct repeat	Right flank	DR Length
TAATACATGAC	TTAA	TCTCTCTGAAT	4

DNA sequence

GAGGGTGTTGCACAACTCAGATCCCCCCATAATTTTTTAACTAAGGTCAATCAAAAGTACTTTACCTTAGTACGTCCAACATATACTCAATGTATAATAC
GATAGGTCGGGATTTTGAACAACTTTACCTTCTCCCTGAGGATATTAGAGACTGGGTTCCGAGTGATGATTTCTGCCATATATTAATTGATTTAATCTCA
ATTCTCGACCTATCCCCATTATATAAGGAATACCGGGAAGACGGCCAAGGTGCGGCATTTTATCATCCAGAAATAATGACTGGTTTGATCATTTACTCAT
ATTTTAATGGAGTTCGTTCAAGCCGTCAAATAGAGAATAAATGCAGATACGATGTAGGATTTAGAATAGTAACCCGCAATTCTCTCCCAGACCATTCCAC
AATCTCGCGATTTATCAAGAAAAACAGTTCTTTTATTGGACAACTTTTCATTCCAGTATTGACTCTCCTTAATGAAGCAGGATTAATCAATAACGTCCTT
CTCGCCTTGGATGGAACAAAACTAAAGGCGAATGCTTCACTTGGATCGAACATGCCATATGAAAAGATTGAGGGAGAAATTCAAAAATATCTAAAGGAAG
TCCAAGAAAAAGATGATGTCGAGGATCGTCTTTATGGCCCGGATAAATCAGGTAACGAGGTTCCCGATGATTTTAAGACTCACTCAAAAAGGATAAAACG
ATTCATTCAAGCAAAAGAACGACTTGAGGCTGAACATAAGGAGAAATCCCAAAAAAAGGAAGAGAAGATTGCCCAAAGGGAGGAAGAGGAGAGAGAGTCT
GGAAAAAGGAAACGAGGTAGAAAACCTAAAAAGCCTGCAAAAAACCCTGATGAGCAAAGTCTTGCAAACACTACAGATCCTGATAGTTCAATAATGAAAA
GTGGGCCTGGTTGTATTCAAGGATATAATGGCCAGATAATTGTAAACCAGGATGGCTATATTCTGGTTCCGTTTTTAAGTAATTCACCCGTTGATTACAG
ATTACTTCAACCATGTTATGAAAGATTAGAGGAAATTGCTCAATTAACCGGCTTATCGCTTGAATATCTTAATTTACTTACTGATGCTGGTTATTGGAGC
TATGAAAACTATCTGTATATGAAACAGCAGGATATTGGATTTCTCTGTTCTACATGCCATGAACCAGATATTTTCAGTATTAGAGGCCTCGAGAGAAGTT
TACTTGAACTTGAACGAATTTCCGATCAACTTTTTGATGGCATTTGCAGTATTCCAACGTTAGCATCTATTGGTGATTGGTGTACGAGAAATCTCATTCA
AGATGATAATATTCCAACACCCGCTTCTATTGCTAAAGGGATTATGGAAGTGACAATGACTCCGTATGGTGCCAAAAAGACATATTCACAACGAAAAACG
ATTGTTGAACCCGCATTTGGATGGATTAAAGAAAATCGCAATTTAAGAAAATTGCAGAGAAGGGGAATATCACATTGTCAGGATGAATGGAGTTTGATTT
GTTTAACACAGAATCTTCGGAAGGTCTGTTCAAGAGGGGAGTTGAAGAAATTCAGAGAACTGATCCTGCAAAAGAAGCGGCATATTATCTGTAATAAGGG
AGTGGGTATTAGAATACCTTATTCAATATTGTCCGATGCATTATCATCTTTGGGCAGAATGATCGGAGACAATTTTCTAAAGATGTTGATAAGAAAAAGT
ACTTACCAAAAATTTTCCAAATTTTAGTCATGCAACACCCTC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1638 bp	545 aa	90	1727	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MYNTIGRDFEQLYLLPEDIRDWVPSDDFCHILIDLISILDLSPLYKEYREDGQGAAFYHPEIMTGLIIYSYFNGVRSSRQIENKCRYDVGFRIVTRNSLP
DHSTISRFIKKNSSFIGQLFIPVLTLLNEAGLINNVLLALDGTKLKANASLGSNMPYEKIEGEIQKYLKEVQEKDDVEDRLYGPDKSGNEVPDDFKTHSK
RIKRFIQAKERLEAEHKEKSQKKEEKIAQREEEERESGKRKRGRKPKKPAKNPDEQSLANTTDPDSSIMKSGPGCIQGYNGQIIVNQDGYILVPFLSNSP
VDYRLLQPCYERLEEIAQLTGLSLEYLNLLTDAGYWSYENYLYMKQQDIGFLCSTCHEPDIFSIRGLERSLLELERISDQLFDGICSIPTLASIGDWCTR
NLIQDDNIPTPASIAKGIMEVTMTPYGAKKTYSQRKTIVEPAFGWIKENRNLRKLQRRGISHCQDEWSLICLTQNLRKVCSRGELKKFRELILQKKRHII
CNKGVGIRIPYSILSDALSSLGRMIGDNFLKMLIRKSTYQKFSKF

Blast result :

Comments

ISMhu1 is 43% aa simialr to ISMac20.

References

1] ISfinder annotation (2006)
2] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina,T., Hammon,N., Israni,S., Pitluck,S., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Kiss,H., Schmutz,J., Larimer,F., Land,M., Kyrpides,N., Ivanova,N., McInerney,M.J., Brockman,F., Culley,D., Ferry,J.G., Gunsalus,R.P., Morrison,M., Plugge,C., Scholten,J., Stams,A.J.M., Boone,D.R. and Richardson,P. (2006) Direct submission GenBank