ISMdi12
- Family IS110
- Group IS1111
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1371 bp
Ends
IR Length : 12
IRL : tgatggaCTGGATGCCCCCACCCGGCGGAGGCCGGTAATCTGCGTCTCGG
IRR : ----tgtCTGGATGCCCCCCGTCGCGCAAGAGGGTGTTGAGCGGTAACGA
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| AGCCGCCGGGTGT | TGACCAATCGACCCATT | 0 |
DNA sequence
TGATGGACTGGATGCCCCCACCCGGCGGAGGCCGGTAATCTGCGTCTCGGGGGCTCACCATCTGCCGGAGAGCGCACCATGGATCCGATCGCAATCGTCG
GCCTCGACTTGGCCAAGCGCGTCTTCCAAGTGCACGGCGCGCGCGCCGATGGCAGCATCGTCCTGCGCAAGAAGCTGTCGCGCCCGCAGGTTCTCACGTT
TTTCGCCCACCTGCCGGCCTGCGTCGTCGCCATGGAGGCCTGCGCCTCGGCGCACGAGTGGGGACGCGCGATCCGCGAACTGGGCCACGAGGCCCGCCTG
ATCCCGCCCGTCTACGTCAAGCCGCTCATCAAGCGCCAGAAGAACGACGCGGCCGACGCCGAGGCCATTGCCGAGGCGGCCCTGCGCCCGACAATGCGGT
TCGTGGCGGTCAAGAGCGAGAGCCAGCAGGCTCGTGCCATGATCTTCCGGACGCGTGATCTTCTGGTGCGCCAGCGAACCCAGCTGATCGATGCGTTGCG
CGGCCATTTGGCCGAGCACGGTGTCGTGGCGTCACAAGGAGTGACCAACGTGCAGCAGCTCGCTGCAGCGGTCGACGACGTCGCCACGTCGCTCGATCCG
CTCGTCGTCGATTTGAGCCGCCTCTACCACGACCAGATCCGGGATCTCGACGGTCGGATCTCGGAGTTGGAGAAGCGCGTCCTCCAAGAAGCCAAGCGGA
CCGGCGTGACGGCACGCCTGATGACGATGCCAGGCATCGGTCCGGTCACCGCGATGGCGATCGAGACGTTCGCGCCGCCCATGGAGACATTCAAGCGTGG
GCGTGACTTTGCAGCCTGGCTCGGCCTCGTGCCGCGGCAGTTCTCATCGGGCGGAAAGCCGGTCCTGGGGCGCGTCTCCAAAATGGGCCAGCGCGACATC
CGGCGGCTTCTGATCATCGGCGCCATAATGGTCGTTCGGCGGTCGACCCGCAAAGGCGCACCGGCGGGCTCGTGGCTGGCCAGGATGCTGGCGCGCAAAC
CGCGCCTGGTCGTTGCGATCGCGCTGGCCAACAAGATGGCCCGCGCGGCTTGGGCCATGCTGACGAAGAACGAAGACTTCAAGGTTCCGGTGCCGACGGC
TTGATCGCTCGATGAATGTTGTAGCTTGGCATCGGTGCGTGGGAAGGTTGAAGAGGGTTAAGGACGGAACGATCGGTCAGATCGGGATCAGAAAAGCAAG
ACTGGTTGACGAGCCTTGAGCTCGCTTGTGTTGATCTGCACCTGATCCGCGCATCTCCATGCTGGCCCGCGGCATGGACATGGCCGCGACGAGAGGCCTG
AGACATGACCGCACCCGATCCTACGTCGTTACCGCTCAACACCCTCTTGCGCGACGGGGGGCATCCAGACA
GCCTCGACTTGGCCAAGCGCGTCTTCCAAGTGCACGGCGCGCGCGCCGATGGCAGCATCGTCCTGCGCAAGAAGCTGTCGCGCCCGCAGGTTCTCACGTT
TTTCGCCCACCTGCCGGCCTGCGTCGTCGCCATGGAGGCCTGCGCCTCGGCGCACGAGTGGGGACGCGCGATCCGCGAACTGGGCCACGAGGCCCGCCTG
ATCCCGCCCGTCTACGTCAAGCCGCTCATCAAGCGCCAGAAGAACGACGCGGCCGACGCCGAGGCCATTGCCGAGGCGGCCCTGCGCCCGACAATGCGGT
TCGTGGCGGTCAAGAGCGAGAGCCAGCAGGCTCGTGCCATGATCTTCCGGACGCGTGATCTTCTGGTGCGCCAGCGAACCCAGCTGATCGATGCGTTGCG
CGGCCATTTGGCCGAGCACGGTGTCGTGGCGTCACAAGGAGTGACCAACGTGCAGCAGCTCGCTGCAGCGGTCGACGACGTCGCCACGTCGCTCGATCCG
CTCGTCGTCGATTTGAGCCGCCTCTACCACGACCAGATCCGGGATCTCGACGGTCGGATCTCGGAGTTGGAGAAGCGCGTCCTCCAAGAAGCCAAGCGGA
CCGGCGTGACGGCACGCCTGATGACGATGCCAGGCATCGGTCCGGTCACCGCGATGGCGATCGAGACGTTCGCGCCGCCCATGGAGACATTCAAGCGTGG
GCGTGACTTTGCAGCCTGGCTCGGCCTCGTGCCGCGGCAGTTCTCATCGGGCGGAAAGCCGGTCCTGGGGCGCGTCTCCAAAATGGGCCAGCGCGACATC
CGGCGGCTTCTGATCATCGGCGCCATAATGGTCGTTCGGCGGTCGACCCGCAAAGGCGCACCGGCGGGCTCGTGGCTGGCCAGGATGCTGGCGCGCAAAC
CGCGCCTGGTCGTTGCGATCGCGCTGGCCAACAAGATGGCCCGCGCGGCTTGGGCCATGCTGACGAAGAACGAAGACTTCAAGGTTCCGGTGCCGACGGC
TTGATCGCTCGATGAATGTTGTAGCTTGGCATCGGTGCGTGGGAAGGTTGAAGAGGGTTAAGGACGGAACGATCGGTCAGATCGGGATCAGAAAAGCAAG
ACTGGTTGACGAGCCTTGAGCTCGCTTGTGTTGATCTGCACCTGATCCGCGCATCTCCATGCTGGCCCGCGGCATGGACATGGCCGCGACGAGAGGCCTG
AGACATGACCGCACCCGATCCTACGTCGTTACCGCTCAACACCCTCTTGCGCGACGGGGGGCATCCAGACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1026 bp | 341 aa | 79 | 1104 | + | No |
Chemistry : DEDD
ORF sequence :
MDPIAIVGLDLAKRVFQVHGARADGSIVLRKKLSRPQVLTFFAHLPACVVAMEACASAHEWGRAIRELGHEARLIPPVYVKPLIKRQKNDAADAEAIAEA
ALRPTMRFVAVKSESQQARAMIFRTRDLLVRQRTQLIDALRGHLAEHGVVASQGVTNVQQLAAAVDDVATSLDPLVVDLSRLYHDQIRDLDGRISELEKR
VLQEAKRTGVTARLMTMPGIGPVTAMAIETFAPPMETFKRGRDFAAWLGLVPRQFSSGGKPVLGRVSKMGQRDIRRLLIIGAIMVVRRSTRKGAPAGSWL
ARMLARKPRLVVAIALANKMARAAWAMLTKNEDFKVPVPTA
ALRPTMRFVAVKSESQQARAMIFRTRDLLVRQRTQLIDALRGHLAEHGVVASQGVTNVQQLAAAVDDVATSLDPLVVDLSRLYHDQIRDLDGRISELEKR
VLQEAKRTGVTARLMTMPGIGPVTAMAIETFAPPMETFKRGRDFAAWLGLVPRQFSSGGKPVLGRVSKMGQRDIRRLLIIGAIMVVRRSTRKGAPAGSWL
ARMLARKPRLVVAIALANKMARAAWAMLTKNEDFKVPVPTA
Blast result :
Comments
ISMdi12 is 67% aa similar to ISRle1.IR are not at the termini, in the IS sequence as given 7 nt separate IRl from the left-hand end of the element and 3 nt separate IRr from the right-hand end.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.