ISMex12

  • Family IS110
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
Methylobacterium chloromethanicum CM4 plasmid pMCHL01
DNA section
IS Length : 1418 bp

Ends


Left end : GGCGGTCATGGCCGATCGGTAGGTCTCTAGAGTGACGGTGTTCGAAGCCTCATTCCGGAGACGGATCGACCATGATCAGACCACAGCTTACGCCGCCGGC
Right end : AACCCCTCTCAGCAGAGCCGTGAGGGCGTGAGAACCACGACCCTGTAGATAATGCTCGGGTCTCCTGCCTGGATCGACATCTCGTGTTGAGGACGGTGAG

Insertion site


Left flankDirect repeatRight flankDR Length
CGCGCCGCCCTGAGGTGGCGCC2

DNA sequence

GGCGGTCATGGCCGATCGGTAGGTCTCTAGAGTGACGGTGTTCGAAGCCTCATTCCGGAGACGGATCGACCATGATCAGACCACAGCTTACGCCGCCGGC
CGGCGTTCTCGTAGCCATCGACGTCGGCAAGGCCCGCAACGAGGTCCTCATCGAGGTGCCCGGCTCCGGCCGACGACGCCGGTTGAGCGTCCTCAACACC
CGCGCCGAGCACGACCGTCTGATCGCGCTGCTGTCGAACCTCGGTCAGCCGGTGACCTGCGGCTTCGAGGCCACCGGCAACTATCACCGCCCGATCGCCT
GGCGCCTGCTCCAGGCCGGCTTTACCGTGCGCCTGATCTCCTCCGTCGGGCTCGCCCGCACCCGCGAGGCCCTGCACAACGGCTGGGACAAGAACGACCC
GAAGGACGCCCAGGTCATGCTGCACATGATCCGCATCGGCGCCTCGCAGGTGTACCACGACCCGCTCGCCGCCGGTTTCAACGACGTGCAGGAACTGTCC
AAGACGCACGACGCCGTCTCGCGGGCCAAGACCGAGGTGCTGCATCGGATCTTGACCCACTACCTGCCGCTGTACTTCCCGGAGGTCGACCGCTTCCGCC
ACAACAGCCGCTCGGACTGGTTCTTCGCCTTCCTCGATCGCTTCCCCACGCCCGCCAGCATCACCGCGCTGAGCAAGGAGGCCTTCATCGCTGCCGCCTG
GGATGTCGTCGGGCGCAAGGTCGCCAAGGCGCAACTGCTCGGCGACATCTACGAGACGGCCCGCAGCTCTATCGGCCTGCCGGTGCCGCCCGATGCTCCG
GCGGTGAGCATGTTCCGGCTCGTCATCGGCGAGGTGCGCGGGCTGATCCAGCAGCGCGACGCCATCGAGGCCACGGCCGACGCCCTCCTGAGGGACAGCT
CCGACTACAAGCTGCTGCGCCGCATCCCTGGCATCGGCCCGATCAACGCCCTGACCATCTTGGCTGAGGCCGGCGACCTGCGCCGCTTCGGCCATCACCG
GCAGTTCCTGAAGTTCTGCGGGCTCGATCTGGCTACCCAGCAATCCGGCACCTTCCGCGGCCAGACCAAGCTGTCGAAGTACGGCAACGCCCGGCTCCGG
CGTACGCTCTGGCTGGCTGGGCAGGTGGCCATCCGGCAGCGGGAGAACGGCTTCCGCGACAAGTTCGAGCGCTACATCGCCCGCGACCGGCACGATGCCG
ACCTGCGGCGCAAGGCGCTGACCGCGATCACCGCCAAGATGGCGCGCGTGGTGCACGCCGTCATCAAGACCGGTGCCGACTACCGGCCCTTCGTCGAGGG
GCCGGTGCCAGGTGGGAGAACCCCTCTCAGCAGAGCCGTGAGGGCGTGAGAACCACGACCCTGTAGATAATGCTCGGGTCTCCTGCCTGGATCGACATCT
CGTGTTGAGGACGGTGAG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1278 bp425 aa721349+No
ORF function : Transposase
Chemistry : DEDD

ORF sequence :

MIRPQLTPPAGVLVAIDVGKARNEVLIEVPGSGRRRRLSVLNTRAEHDRLIALLSNLGQPVTCGFEATGNYHRPIAWRLLQAGFTVRLISSVGLARTREA
LHNGWDKNDPKDAQVMLHMIRIGASQVYHDPLAAGFNDVQELSKTHDAVSRAKTEVLHRILTHYLPLYFPEVDRFRHNSRSDWFFAFLDRFPTPASITAL
SKEAFIAAAWDVVGRKVAKAQLLGDIYETARSSIGLPVPPDAPAVSMFRLVIGEVRGLIQQRDAIEATADALLRDSSDYKLLRRIPGIGPINALTILAEA
GDLRRFGHHRQFLKFCGLDLATQQSGTFRGQTKLSKYGNARLRRTLWLAGQVAIRQRENGFRDKFERYIARDRHDADLRRKALTAITAKMARVVHAVIKT
GADYRPFVEGPVPGGRTPLSRAVRA

 

Blast result :
Comments
ISMex12 is 64% aa similar to ISYps2.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.