ISMex33
- Family IS3
- Group IS51
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1318 bp
Ends
IR Length : 22/26
IRL : TGAACCGCCCCGGGATTTTCGGAGGCTCCAACTCTTGAGAGACTGGAGCC
IRR : TGAACCGCGCCGGGTTTCCCGGAGGCCGTTTGGTTTGGGTCAGGCGGCCA
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
GAGTCCGCTT | TCCCGTCGAC | 0 |
DNA sequence
TGAACCGCCCCGGGATTTTCGGAGGCTCCAACTCTTGAGAGACTGGAGCCATGACGAAGCATACCCCACCGTTTTCCCCCGAAGTCCGCGAGCGGGCAGT
CCGGCTGGCCCGGGAGCATGAGAGCGAGCACGGTTCGCAATGGGCCGCGATCCAGTCGATCGCGGCCAAAATCGGTTGCTCTGGCGAGACGCTGCGCAAG
TGGGTCCGCCAGGCCGAGCGCGACCGGGGCGTGCGGGCCGGACCGACGACGGATGAGCGCGAGCGGATCAAGGCGCTGGAGCGGGAGAACCGCGAGCTCC
GGCAGGCCAACGAGATCCTCAGGAAGGCGAGCGCGTATTTTGCCCAGGCGGAGCTCGACCGCCGGTTCCGGTCATGATCGCCTTCATCGACGATCATCGT
GCGCTCTACGGGGTCGAGCCGATCTGCAAGGTTCTGCCGATCGCCCCGTCGACGTACCACGCCCATGCCGCGAGACGAGCCGATCCCGAGAGGATGCCGG
CTCGAGCCAAGCGGGACGCGGCACTCATGGTCGAGATCCGGCGCGTGTACGAGGCGAACTTCCGGGTCTACGGCGTGCGCAAAGTCTGGCGGCAACTCGC
CCGCGACGGGATCGTTGTGGCGCGCTGCACGGTGGCCCGACTGATGCGCAGCATGGGCTTGGCCGGCGTCGTCCGGGGGCGCCGGGTCCGCACCACCGTT
CCCAATCCGGCTGCGGCCTGCCCCCTCGACCGGGTCAACCGGCAGTTCAAGGCCGAGTGCCCGAACCGGCTGTGGGTCGCAGATTTTACCTACGTGGCGA
CATGGGCCGGCTTCGTCTATGCGGCCTTCGTGATCGACGTTTTCGCTCGGCGGATTGTGGGCTGGCGTGTCTCACGCTCGGCTCGGGCGGACTTCGTGCT
CGATGCCCTGGAGCAGGCTCTGCACGAGCGCCGCCCCTTCGCCGGCAGCGGCCTCGTCTGCCACTCGGATCGCGGGTCGCAATACGTGAGCATCCGCTAC
ACCGAGCGCCTCGCCGAGGCGGGCGTCGAGCCCTCCGTCGGCAGCGTCGGCGATTCGTACGATAACGCTCTGGCCGAGACGGTGATCGGCCTGTTCAAGG
CGGAGGTGATCCATCGACGCGGGCCGTGGCGCTCGTTCGAGGCAGTCGAGTACGCCACGCTCGAATGGGTGGATTGGTTCAATCACCGTCGGCTGCTCGA
ACCGATCGGTCACATCCCTCCCGCCGAGGCGGAGACGCGCTATTATGCTCAGGCTGAGGTCGAAGCCTTGGCCGCCTGACCCAAACCAAACGGCCTCCGG
GAAACCCGGCGCGGTTCA
CCGGCTGGCCCGGGAGCATGAGAGCGAGCACGGTTCGCAATGGGCCGCGATCCAGTCGATCGCGGCCAAAATCGGTTGCTCTGGCGAGACGCTGCGCAAG
TGGGTCCGCCAGGCCGAGCGCGACCGGGGCGTGCGGGCCGGACCGACGACGGATGAGCGCGAGCGGATCAAGGCGCTGGAGCGGGAGAACCGCGAGCTCC
GGCAGGCCAACGAGATCCTCAGGAAGGCGAGCGCGTATTTTGCCCAGGCGGAGCTCGACCGCCGGTTCCGGTCATGATCGCCTTCATCGACGATCATCGT
GCGCTCTACGGGGTCGAGCCGATCTGCAAGGTTCTGCCGATCGCCCCGTCGACGTACCACGCCCATGCCGCGAGACGAGCCGATCCCGAGAGGATGCCGG
CTCGAGCCAAGCGGGACGCGGCACTCATGGTCGAGATCCGGCGCGTGTACGAGGCGAACTTCCGGGTCTACGGCGTGCGCAAAGTCTGGCGGCAACTCGC
CCGCGACGGGATCGTTGTGGCGCGCTGCACGGTGGCCCGACTGATGCGCAGCATGGGCTTGGCCGGCGTCGTCCGGGGGCGCCGGGTCCGCACCACCGTT
CCCAATCCGGCTGCGGCCTGCCCCCTCGACCGGGTCAACCGGCAGTTCAAGGCCGAGTGCCCGAACCGGCTGTGGGTCGCAGATTTTACCTACGTGGCGA
CATGGGCCGGCTTCGTCTATGCGGCCTTCGTGATCGACGTTTTCGCTCGGCGGATTGTGGGCTGGCGTGTCTCACGCTCGGCTCGGGCGGACTTCGTGCT
CGATGCCCTGGAGCAGGCTCTGCACGAGCGCCGCCCCTTCGCCGGCAGCGGCCTCGTCTGCCACTCGGATCGCGGGTCGCAATACGTGAGCATCCGCTAC
ACCGAGCGCCTCGCCGAGGCGGGCGTCGAGCCCTCCGTCGGCAGCGTCGGCGATTCGTACGATAACGCTCTGGCCGAGACGGTGATCGGCCTGTTCAAGG
CGGAGGTGATCCATCGACGCGGGCCGTGGCGCTCGTTCGAGGCAGTCGAGTACGCCACGCTCGAATGGGTGGATTGGTTCAATCACCGTCGGCTGCTCGA
ACCGATCGGTCACATCCCTCCCGCCGAGGCGGAGACGCGCTATTATGCTCAGGCTGAGGTCGAAGCCTTGGCCGCCTGACCCAAACCAAACGGCCTCCGG
GAAACCCGGCGCGGTTCA
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
327 bp | 108 aa | 51 | 377 | + | No |
Description : First part of the transposase
ORF sequence :
MTKHTPPFSPEVRERAVRLAREHESEHGSQWAAIQSIAAKIGCSGETLRKWVRQAERDRGVRAGPTTDERERIKALERENRELRQANEILRKASAYFAQA
ELDRRFRS
ELDRRFRS
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1023 bp | 340 aa | 257 | 1279 | + | No |
Description : Second part of the transposase
ORF sequence :
MIAFIDDHRALYGVEPICKVLPIAPSTYHAHAARRADPERMPARAKRDAALMVEIRRVYEANFRVYGVRKVWRQLARDGIVVARCTVARLMRSMGLAGVV
RGRRVRTTVPNPAAACPLDRVNRQFKAECPNRLWVADFTYVATWAGFVYAAFVIDVFARRIVGWRVSRSARADFVLDALEQALHERRPFAGSGLVCHSDR
GSQYVSIRYTERLAEAGVEPSVGSVGDSYDNALAETVIGLFKAEVIHRRGPWRSFEAVEYATLEWVDWFNHRRLLEPIGHIPPAEAETRYYAQAEVEALA
A
RGRRVRTTVPNPAAACPLDRVNRQFKAECPNRLWVADFTYVATWAGFVYAAFVIDVFARRIVGWRVSRSARADFVLDALEQALHERRPFAGSGLVCHSDR
GSQYVSIRYTERLAEAGVEPSVGSVGDSYDNALAETVIGLFKAEVIHRRGPWRSFEAVEYATLEWVDWFNHRRLLEPIGHIPPAEAETRYYAQAEVEALA
A
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
1229 bp | 409 aa | 51 | 1279 | + | Yes |
Chemistry : DDE
ORF sequence :
MTKHTPPFSPEVRERAVRLAREHESEHGSQWAAIQSIAAKIGCSGETLRKWVRQAERDRGVRAGPTTDERERIKALERENRELRQANEILRKASAYFCPG
GARPPVPVMIAFIDDHRALYGVEPICKVLPIAPSTYHAHAARRADPERMPARAKRDAALMVEIRRVYEANFRVYGVRKVWRQLARDGIVVARCTVARLMR
SMGLAGVVRGRRVRTTVPNPAAACPLDRVNRQFKAECPNRLWVADFTYVATWAGFVYAAFVIDVFARRIVGWRVSRSARADFVLDALEQALHERRPFAGS
GLVCHSDRGSQYVSIRYTERLAEAGVEPSVGSVGDSYDNALAETVIGLFKAEVIHRRGPWRSFEAVEYATLEWVDWFNHRRLLEPIGHIPPAEAETRYYA
QAEVEALAA
GARPPVPVMIAFIDDHRALYGVEPICKVLPIAPSTYHAHAARRADPERMPARAKRDAALMVEIRRVYEANFRVYGVRKVWRQLARDGIVVARCTVARLMR
SMGLAGVVRGRRVRTTVPNPAAACPLDRVNRQFKAECPNRLWVADFTYVATWAGFVYAAFVIDVFARRIVGWRVSRSARADFVLDALEQALHERRPFAGS
GLVCHSDRGSQYVSIRYTERLAEAGVEPSVGSVGDSYDNALAETVIGLFKAEVIHRRGPWRSFEAVEYATLEWVDWFNHRRLLEPIGHIPPAEAETRYYA
QAEVEALAA
Blast result :
Comments
ISMex33 has 1 intact copy in AM1 and 1 partial copy in DM4.
ISMex33 is 85% aa similar to ISRtr2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISMex33 is 85% aa similar to ISRtr2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Stéphane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS ONE Submitted. (2009)