ISMex33

  • Family IS3
  • Group IS51
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
Methylobacterium dichloromethanicum DM4
DNA section
IS Length : 1318 bp

Ends


IR Length : 22/26

IRL : TGAACCGCCCCGGGATTTTCGGAGGCTCCAACTCTTGAGAGACTGGAGCC
IRR : TGAACCGCGCCGGGTTTCCCGGAGGCCGTTTGGTTTGGGTCAGGCGGCCA

Insertion site


Left flankDirect repeatRight flankDR Length
GAGTCCGCTTTCCCGTCGAC0

DNA sequence

TGAACCGCCCCGGGATTTTCGGAGGCTCCAACTCTTGAGAGACTGGAGCCATGACGAAGCATACCCCACCGTTTTCCCCCGAAGTCCGCGAGCGGGCAGT
CCGGCTGGCCCGGGAGCATGAGAGCGAGCACGGTTCGCAATGGGCCGCGATCCAGTCGATCGCGGCCAAAATCGGTTGCTCTGGCGAGACGCTGCGCAAG
TGGGTCCGCCAGGCCGAGCGCGACCGGGGCGTGCGGGCCGGACCGACGACGGATGAGCGCGAGCGGATCAAGGCGCTGGAGCGGGAGAACCGCGAGCTCC
GGCAGGCCAACGAGATCCTCAGGAAGGCGAGCGCGTATTTTGCCCAGGCGGAGCTCGACCGCCGGTTCCGGTCATGATCGCCTTCATCGACGATCATCGT
GCGCTCTACGGGGTCGAGCCGATCTGCAAGGTTCTGCCGATCGCCCCGTCGACGTACCACGCCCATGCCGCGAGACGAGCCGATCCCGAGAGGATGCCGG
CTCGAGCCAAGCGGGACGCGGCACTCATGGTCGAGATCCGGCGCGTGTACGAGGCGAACTTCCGGGTCTACGGCGTGCGCAAAGTCTGGCGGCAACTCGC
CCGCGACGGGATCGTTGTGGCGCGCTGCACGGTGGCCCGACTGATGCGCAGCATGGGCTTGGCCGGCGTCGTCCGGGGGCGCCGGGTCCGCACCACCGTT
CCCAATCCGGCTGCGGCCTGCCCCCTCGACCGGGTCAACCGGCAGTTCAAGGCCGAGTGCCCGAACCGGCTGTGGGTCGCAGATTTTACCTACGTGGCGA
CATGGGCCGGCTTCGTCTATGCGGCCTTCGTGATCGACGTTTTCGCTCGGCGGATTGTGGGCTGGCGTGTCTCACGCTCGGCTCGGGCGGACTTCGTGCT
CGATGCCCTGGAGCAGGCTCTGCACGAGCGCCGCCCCTTCGCCGGCAGCGGCCTCGTCTGCCACTCGGATCGCGGGTCGCAATACGTGAGCATCCGCTAC
ACCGAGCGCCTCGCCGAGGCGGGCGTCGAGCCCTCCGTCGGCAGCGTCGGCGATTCGTACGATAACGCTCTGGCCGAGACGGTGATCGGCCTGTTCAAGG
CGGAGGTGATCCATCGACGCGGGCCGTGGCGCTCGTTCGAGGCAGTCGAGTACGCCACGCTCGAATGGGTGGATTGGTTCAATCACCGTCGGCTGCTCGA
ACCGATCGGTCACATCCCTCCCGCCGAGGCGGAGACGCGCTATTATGCTCAGGCTGAGGTCGAAGCCTTGGCCGCCTGACCCAAACCAAACGGCCTCCGG
GAAACCCGGCGCGGTTCA
Recoding section
  • Recoding by frameshift
  • Frame
  • Type
  • Experimentally demonstrated

Stimulators :

  • Shine-Dalgarno sequence :
  • Secondary structure :

Recoding motif :

Protein section
ORF number : 3

 

ORF 1
LengthBeginEndStrandFusion ORF
327 bp108 aa51377+No
ORF function : Transposase
Description : First part of the transposase

ORF sequence :

MTKHTPPFSPEVRERAVRLAREHESEHGSQWAAIQSIAAKIGCSGETLRKWVRQAERDRGVRAGPTTDERERIKALERENRELRQANEILRKASAYFAQA
ELDRRFRS

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
1023 bp340 aa2571279+No
ORF function : Transposase
Description : Second part of the transposase

ORF sequence :

MIAFIDDHRALYGVEPICKVLPIAPSTYHAHAARRADPERMPARAKRDAALMVEIRRVYEANFRVYGVRKVWRQLARDGIVVARCTVARLMRSMGLAGVV
RGRRVRTTVPNPAAACPLDRVNRQFKAECPNRLWVADFTYVATWAGFVYAAFVIDVFARRIVGWRVSRSARADFVLDALEQALHERRPFAGSGLVCHSDR
GSQYVSIRYTERLAEAGVEPSVGSVGDSYDNALAETVIGLFKAEVIHRRGPWRSFEAVEYATLEWVDWFNHRRLLEPIGHIPPAEAETRYYAQAEVEALA
A

 

Blast result :
ORF 3
LengthBeginEndStrandFusion ORF
1229 bp409 aa511279+Yes
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MTKHTPPFSPEVRERAVRLAREHESEHGSQWAAIQSIAAKIGCSGETLRKWVRQAERDRGVRAGPTTDERERIKALERENRELRQANEILRKASAYFCPG
GARPPVPVMIAFIDDHRALYGVEPICKVLPIAPSTYHAHAARRADPERMPARAKRDAALMVEIRRVYEANFRVYGVRKVWRQLARDGIVVARCTVARLMR
SMGLAGVVRGRRVRTTVPNPAAACPLDRVNRQFKAECPNRLWVADFTYVATWAGFVYAAFVIDVFARRIVGWRVSRSARADFVLDALEQALHERRPFAGS
GLVCHSDRGSQYVSIRYTERLAEAGVEPSVGSVGDSYDNALAETVIGLFKAEVIHRRGPWRSFEAVEYATLEWVDWFNHRRLLEPIGHIPPAEAETRYYA
QAEVEALAA

 

Blast result :
Comments
ISMex33 has 1 intact copy in AM1 and 1 partial copy in DM4.
ISMex33 is 85% aa similar to ISRtr2.
The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
References
1] Stéphane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS ONE Submitted. (2009)