ISMex15
- Family IS5
- Group IS427
Isoform Synonym(s)
Accession number | Transposition | Origin | Host |
---|---|---|---|
ND | Methylobacterium extorquens | Methylobacterium extorquens AM1 Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 832 bp
Ends
IR Length : 15/16
IRL : GGCTCCGGACCCATAAGTTTGGCTTGGCGGGTGTGCCTGTGATTTCTTCC
IRR : GGCTCCGGACTCATAACCAGTAGCTGACGGTAGCGGCGATGCAGACGGCC
Insertion site
Left flank | Direct repeat | Right flank | DR Length |
---|---|---|---|
CAACGGATTT | TA | GGACTATGGA | 2 |
TTTCGACCGC | TA | GGATCGTAGG | 2 |
GCAGAGCGGGATAC | TA | ATACCCTAGCCTCT | 2 |
GACGCTCCGC | TA | GTGCGTCGAG | 2 |
TGCTTTGCCC | TA | AGATACCTGC | 2 |
CCAACACCAC | TA | AGGCCTGCTA | 2 |
AACACCGAGC | TA | GGCCGACCGA | 2 |
ACTGTCATCG | TA | GTGCAGCGCC | 2 |
GAGCGGTGCC | TA | GGGCTCGCCG | 2 |
TCCTGACCGT | TA | GAGCGAGATC | 2 |
AACACCGGAT | TA | GTTCTGCCCA | 2 |
DNA sequence
GGCTCCGGACCCATAAGTTTGGCTTGGCGGGTGTGCCTGTGATTTCTTCCGGGTGAGGGAGGAGCCAATGGGCGAGTTCTGGTTGACCGACGAGCAGTTT
GCAAAGATCGCACCGCACCTTCCGACAGACACCCGGGGTAAGGAGCGCGTCGACGACCGACGTGTGATCAGCGGGATCATCCACGTGTTGAAGTCGGGCG
GGCGTTGGACGGACGCCCCGCGCGAGATCTACGGCCCGAAGAAGACCCTCTACAATCGCTTCGTGCGCTGGGCGGCCAAGGGGATCTGGAGGGATCTGTT
CGAGACCCTGGCCCGGGCCGGTGGGCCACCCTCCCAGGTGCTGATCGACTCCTCGGCCGTGAAGGCGCATCGCTGCGCCTCCGGTGGAAAGGGGGGGAGC
AAAGCCATGCCATCGGCCGCTCGCGGGGTGGACGCACCACCAAGATCCACGCGCTCACCGACCGGTTCTGCCGCCCGCTCGCCTTCCTGCTGACGGGCGG
TCAGGTGGCGGACTGCAAGGCGGGGGCACAGCTCTTGGAGGGCGCGCCAGCGTGCCGGATCATGTTGGCCGACAAGGGCTACGACAGCGACGCCATCCGC
CGTCAGATCGAGGCGACGGGGGCGGCGCCCAACATCCCGCCCAAGTCCAACCGGCGATGGAAGCCGTGCTTCTCCCCCGTCCTGTACAAGGGCCGCAACG
CCATCGAGCGGATGTTCGGGCGCCTCAAGGACTTCCGCCGCATCGCCACCCGATACGACCGGCTGGCAGCCAACTACCTGGCGGCCGTCTGCATCGCCGC
TACCGTCAGCTACTGGTTATGAGTCCGGAGCC
GCAAAGATCGCACCGCACCTTCCGACAGACACCCGGGGTAAGGAGCGCGTCGACGACCGACGTGTGATCAGCGGGATCATCCACGTGTTGAAGTCGGGCG
GGCGTTGGACGGACGCCCCGCGCGAGATCTACGGCCCGAAGAAGACCCTCTACAATCGCTTCGTGCGCTGGGCGGCCAAGGGGATCTGGAGGGATCTGTT
CGAGACCCTGGCCCGGGCCGGTGGGCCACCCTCCCAGGTGCTGATCGACTCCTCGGCCGTGAAGGCGCATCGCTGCGCCTCCGGTGGAAAGGGGGGGAGC
AAAGCCATGCCATCGGCCGCTCGCGGGGTGGACGCACCACCAAGATCCACGCGCTCACCGACCGGTTCTGCCGCCCGCTCGCCTTCCTGCTGACGGGCGG
TCAGGTGGCGGACTGCAAGGCGGGGGCACAGCTCTTGGAGGGCGCGCCAGCGTGCCGGATCATGTTGGCCGACAAGGGCTACGACAGCGACGCCATCCGC
CGTCAGATCGAGGCGACGGGGGCGGCGCCCAACATCCCGCCCAAGTCCAACCGGCGATGGAAGCCGTGCTTCTCCCCCGTCCTGTACAAGGGCCGCAACG
CCATCGAGCGGATGTTCGGGCGCCTCAAGGACTTCCGCCGCATCGCCACCCGATACGACCGGCTGGCAGCCAACTACCTGGCGGCCGTCTGCATCGCCGC
TACCGTCAGCTACTGGTTATGAGTCCGGAGCC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
426 bp | 141 aa | 68 | 493 | + | No |
Description : First part of the transposase
ORF sequence :
MGEFWLTDEQFAKIAPHLPTDTRGKERVDDRRVISGIIHVLKSGGRWTDAPREIYGPKKTLYNRFVRWAAKGIWRDLFETLARAGGPPSQVLIDSSAVKA
HRCASGGKGGSKAMPSAARGVDAPPRSTRSPTGSAARSPSC
HRCASGGKGGSKAMPSAARGVDAPPRSTRSPTGSAARSPSC
Blast result :ORF 2
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
555 bp | 184 aa | 268 | 822 | + | No |
Description : Second part of the transposase
ORF sequence :
LGGQGDLEGSVRDPGPGRWATLPGADRLLGREGASLRLRWKGGEQSHAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQVADCKAGAQLLEGAPACRIML
ADKGYDSDAIRRQIEATGAAPNIPPKSNRRWKPCFSPVLYKGRNAIERMFGRLKDFRRIATRYDRLAANYLAAVCIAATVSYWL
ADKGYDSDAIRRQIEATGAAPNIPPKSNRRWKPCFSPVLYKGRNAIERMFGRLKDFRRIATRYDRLAANYLAAVCIAATVSYWL
Blast result :ORF 3
Length | Begin | End | Strand | Fusion ORF | |
---|---|---|---|---|---|
755 bp | 250 aa | 68 | 822 | + | Yes |
Chemistry : DDE
ORF sequence :
MGEFWLTDEQFAKIAPHLPTDTRGKERVDDRRVISGIIHVLKSGGRWTDAPREIYGPKKTLYNRFVRWAAKGIWRDLFETLARAGGPPSQVLIDSSAVKA
HRCASGGKGGQSHAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQVADCKAGAQLLEGAPACRIMLADKGYDSDAIRRQIEATGAAPNIPPKSNRRWKPC
FSPVLYKGRNAIERMFGRLKDFRRIATRYDRLAANYLAAVCIAATVSYWL
HRCASGGKGGQSHAIGRSRGGRTTKIHALTDRFCRPLAFLLTGGQVADCKAGAQLLEGAPACRIMLADKGYDSDAIRRQIEATGAAPNIPPKSNRRWKPC
FSPVLYKGRNAIERMFGRLKDFRRIATRYDRLAANYLAAVCIAATVSYWL
Blast result :
Comments
ISMex15 is 84% aa similar to ISSen2.
The third ORF is a putative ORFAB transposase reconstructed in silico by -1 programmed frameshift.
The third ORF is a putative ORFAB transposase reconstructed in silico by -1 programmed frameshift.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009)PLoS ONE Submitted.