ISMex39

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
DNA section
IS Length : 2456 bp

Ends


IR Length : 27/35

IRL : TGACCCTGGATCGGCGTCCAATTCTGACCCCCTAACCTCGTTCTCGCAGA
IRR : TGAGGCGGGGGATCGGCGTCGAACTTTGACCCCCTGGATGGGTATGGCGG

Insertion site


Left flankDirect repeatRight flankDR Length
CATA0

DNA sequence

TGACCCTGGATCGGCGTCCAATTCTGACCCCCTAACCTCGTTCTCGCAGACTGCCCTCTGGTGCGGCCGGAAGGGTGGTGCAGGGGGATGAAGGGCGTGG
ACACGATTGCGCGCATCCGGCGCGAGTTCTTCACCCGCGGCCGCGCGATCAAGGACATCGTCCGCGACCTGCACGTCTCCCGCAACACGGTCCGGAAGGT
CATCCGGTCGGGGGCCACCGCCTTCACCTACGAGCGCGACGTGCAGCCTCTGCCCAAGCTCGGGCCGTGGCGCGACGATCTCGATCGGATGCTGACCACC
AACGCCGGCAGGTCCGCCCGCGAGCGACTGACGCTGATCCGGATCTACGAGGCGCTGCGCGGGCTCGGCTACGAGGGCGGCTACGATGCCGTTCGGCGCT
ATGCCAAAACCTGGAAGCGCGAGCGCGCCTCGGTCACGGCGCAGGCCTTCGTGCCGCTCATCTTCGCCCCAGGCGAGGCCTACCAGTTCGACTGGAGCCA
CGAGATCGTGCTGATCGGCGGCACGACCGTCACGGTCAAGGTCGCTCACGTCCGGCTCTGCCACTCGCGCATGCTGTTCGTGCGGGCTTATCCGCGCGAG
AGCCAGGAGATGGTGTTCGACGCCCACGACCGGGCGTTCGCGTTCTTCCGCGGCACCTGCCAGCGCGGCATCTACGACAACATGAAGACCGCGGTGGAGA
CGATCTTCGTCGGTCGCGAGCGCGCCTACAACCGTCGCTTCCTGCAGATGTGCTCGCACTACCTCGTCGATCCGGTCGCCTGCACGCCGGCCTCGGGCTG
GGAGAAGGGGCAGGTCGAGAACCAGGTCGGGCTGGTGCGCGAGCGCTTCTTCACCCCGCGCGTCCGGGTGAAGAGCTACGACGAGTTGAACGCGCTCCTA
CTCGATGGAGCTATCGCCTACGCCAAGGCCCATCCGCATCCCGAGGAGCGCGAGCGCACCGTCTGGGAGCGGTTCGAGGCCGAGCGGGCGGCGCTGGTGC
CCTATGCTGGCCGCTTCGACGGCTTCCACGCCGTCACCGCTTCGGTGTCCGCGACCTGCCTCGTACGCTTCGACAACAACCGATACTCGGTCATGGCCTC
CGCCATTGGCCGCCCGGTCGAAGTGCGAGCCTACGCCGAGCGCATCGAGATCCGGCAGGACGGGCGCGTCGTCGCCGAGCACGCGCGTGCCTTCGGCCGG
GATCAGACCGTGTTCGATCCCTGGCACTACGTCCCTGTGCTCGCCCGCAAACCTGGCGCGCTCCGGAACGGCGCACCGTTCAAGGACTGGGTGCTGCCCG
CCGCCCTCGACCGGGTTCGACGCAAGCTCGCCGGCAGTGCCGGGGGTGACCGGCAGATGGTCGAGATCCTCACCGCTGTGCTCGGTGACGGGTTGCCGGC
GGTCGAGGCGGCCTGTGCCGAGGCCCTGCGCGAGGGCGTCCACTCGGCCGATGTCGTCCTCAACATCCTCGCCCGGCAGCGCGAGCCGACCACGCCCGTC
ACCATCCTGACGCCCGAGAGCCTGCGGCTGCGCCACGAGCCGGTCGCCGACTGTGCCCGCTACGACAGCCTAAGGAGAGCACCATGATGGAACGCCAGCA
GATCCTCGCCACCATGGGCGATCTGAAGCTCTTCGGCATGAAGGCCGCCTACGACGAGATCATCAAGGTCGCAGTGAAGCGCACCCACGAGCCGCAGCAG
ATCGTCGGCGACCTGCTCCAGGCAGAGATCTCCGAGAAGCAGGCACGCTCGATCCGCTACCAGATGACCATCGCCAAACTGCCCCTGGCCAAGGACATCG
CCGAGTTCGCCTTCGACGGCACTCCGATCAACGAGGTGCTGGTGCGTGATCTCGCCGGCGGCGAGTTCCTGGCGCATCAGCGCAACGTCGTGCTCGTCGG
CGGCACCGGGACCGGCAAGACCCACCTCGCCATCGCCCTTGCACGAGCCTGCATCCGGGATGGCATGCGGGGCCGGTTCTACAACGTCGTCGATCTCGTC
AATCGGCTCGAAGCTGAGGCACGCGCCGGCCGGCACGGCCGCATCGCCGACCATCTGGCGCGGCTCGACTTCGTGGTGCTCGACGAACTCGGCTACCTGC
CGTTCGCGCAGTCGGGCGGCCAACTCCTGTTCCACCTGATCAGCAAGCTCTACGAGACGACCTCGATCGTGGTCACCACCAACCTCGCCTTCGGGGAATG
GCCGAGCGTGTTCGCCGGCGATGCCAAGATGACCACCGCGCTCCTCGACCGGCTCACCCACCACTGCGAGATCGTCGAGACCGGCAACGAGAGCTGGCGC
TTCAAGAACCGCGCCTGAGGCCAGCACACACGTCGCGCCTGCTGGACCCCTGATCAGCCCGGCTGCGCCACCCCGACCCGCCTTCGCCGGGCTGATCAGG
GGCGTCCCGCCATACCCATCCAGGGGGTCAAAGTTCGACGCCGATCCCCCGCCTCA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1527 bp508 aa611587+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

VRPEGWCRGMKGVDTIARIRREFFTRGRAIKDIVRDLHVSRNTVRKVIRSGATAFTYERDVQPLPKLGPWRDDLDRMLTTNAGRSARERLTLIRIYEALR
GLGYEGGYDAVRRYAKTWKRERASVTAQAFVPLIFAPGEAYQFDWSHEIVLIGGTTVTVKVAHVRLCHSRMLFVRAYPRESQEMVFDAHDRAFAFFRGTC
QRGIYDNMKTAVETIFVGRERAYNRRFLQMCSHYLVDPVACTPASGWEKGQVENQVGLVRERFFTPRVRVKSYDELNALLLDGAIAYAKAHPHPEERERT
VWERFEAERAALVPYAGRFDGFHAVTASVSATCLVRFDNNRYSVMASAIGRPVEVRAYAERIEIRQDGRVVAEHARAFGRDQTVFDPWHYVPVLARKPGA
LRNGAPFKDWVLPAALDRVRRKLAGSAGGDRQMVEILTAVLGDGLPAVEAACAEALREGVHSADVVLNILARQREPTTPVTILTPESLRLRHEPVADCAR
YDSLRRAP

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
735 bp244 aa15842318+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MMERQQILATMGDLKLFGMKAAYDEIIKVAVKRTHEPQQIVGDLLQAEISEKQARSIRYQMTIAKLPLAKDIAEFAFDGTPINEVLVRDLAGGEFLAHQR
NVVLVGGTGTGKTHLAIALARACIRDGMRGRFYNVVDLVNRLEAEARAGRHGRIADHLARLDFVVLDELGYLPFAQSGGQLLFHLISKLYETTSIVVTTN
LAFGEWPSVFAGDAKMTTALLDRLTHHCEIVETGNESWRFKNRA

 

Blast result :
Comments
ISMex39 is 69% (istA, the transposase) and 79% (istB, the helper of transposition) aa similar to ISSsp4.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.