ISMdi8
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 |
DNA section
IS Length : 1002 bp
Ends
IR Length : 22/30
IRL : TGTTTGATCCCGCGGTGTGGTGATACGGTTGACCACCATGCTGCGAGGGA
IRR : TGTATGGTCCCGGGATGAGGTGTGACGGGTCGATCCTGAAGCGATTGGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| GTCTGCTAGG | CCTAGGAGCC | 0 | |
| CAAAACTAGG | CCTAGAGACT | 0 |
DNA sequence
TGTTTGATCCCGCGGTGTGGTGATACGGTTGACCACCATGCTGCGAGGGATGCGCTATGGGCCAGGTTCTCCATGGAAGCGCCACCACGACGGAGGCAGT
CCGTCGCGCGATCCAGCTACGTGAAGAGAGCGTAAGGTCGCTGGCCAAGCGCTACGGGGTCAGCCCGACCACGGTGCAGAAGTGGCGCAAACGGACGAGC
ACTGCGGACGCCCGGATGGGCCCCAAGGAGCCGCGCTCCACCGTGCTCACCGTTGAGGAGGAAGCGATCGTTGTCGCCTTCCGCCGGCACACCCTGCTGC
CGCTTGACGACTGCCTCTACGGCTTGCAGCCGACGATCCCGCACCTGACGCGATCCTCGCTGTATCGCTGCTTGGAACGGCATGGGATCAGCAGACTACC
CGAGATCGACGGCGACAAACCCAAGAAGCAGCGCTTTGCAACCTACGCCATCGGCTATGTGCATATCGATATCGCCGAGGTGAGCACGGCCGAAGGCAAG
CTCAGGCTGTTCGTGGCCATTGATCGAACCAGCAAGTTTGCCTTCGTGCGGCTCGTGGAGAGCGCCGGCAAAATGGAGGCAGCGCAGTTCCTGCGCGACT
TCATCGACGCGGTGCCTTACCGCATCCACACCGTGCTCACAGACAACGGCGTTCAGTTCACATCACGCCGACAGGACATCTACGACAGCCAGCACATCTT
CGACCGCGTCTGCGACGAGCATGACATCGAGCATCGGCTGACCAAGGTGAACCATCCTTGGACCAACGGACAAGTCGAGCGGATGAACCGCACGATCAAG
GACGCGACGGTCAAGCGCTACCACTACGATAGCCACGACCAGCTTCGAACGCATCTGCACCTGTTCGTCGATGCCTACAATCACGCTCGTAGGCTCAAGA
CCCTGCGGGGCCTGACACCCACCGAGTTCATCCTGAACGCCTGGACGAAAGAGCCCAATCGCTTCAGGATCGACCCGTCACACCTCATCCCGGGACCATA
CA
CCGTCGCGCGATCCAGCTACGTGAAGAGAGCGTAAGGTCGCTGGCCAAGCGCTACGGGGTCAGCCCGACCACGGTGCAGAAGTGGCGCAAACGGACGAGC
ACTGCGGACGCCCGGATGGGCCCCAAGGAGCCGCGCTCCACCGTGCTCACCGTTGAGGAGGAAGCGATCGTTGTCGCCTTCCGCCGGCACACCCTGCTGC
CGCTTGACGACTGCCTCTACGGCTTGCAGCCGACGATCCCGCACCTGACGCGATCCTCGCTGTATCGCTGCTTGGAACGGCATGGGATCAGCAGACTACC
CGAGATCGACGGCGACAAACCCAAGAAGCAGCGCTTTGCAACCTACGCCATCGGCTATGTGCATATCGATATCGCCGAGGTGAGCACGGCCGAAGGCAAG
CTCAGGCTGTTCGTGGCCATTGATCGAACCAGCAAGTTTGCCTTCGTGCGGCTCGTGGAGAGCGCCGGCAAAATGGAGGCAGCGCAGTTCCTGCGCGACT
TCATCGACGCGGTGCCTTACCGCATCCACACCGTGCTCACAGACAACGGCGTTCAGTTCACATCACGCCGACAGGACATCTACGACAGCCAGCACATCTT
CGACCGCGTCTGCGACGAGCATGACATCGAGCATCGGCTGACCAAGGTGAACCATCCTTGGACCAACGGACAAGTCGAGCGGATGAACCGCACGATCAAG
GACGCGACGGTCAAGCGCTACCACTACGATAGCCACGACCAGCTTCGAACGCATCTGCACCTGTTCGTCGATGCCTACAATCACGCTCGTAGGCTCAAGA
CCCTGCGGGGCCTGACACCCACCGAGTTCATCCTGAACGCCTGGACGAAAGAGCCCAATCGCTTCAGGATCGACCCGTCACACCTCATCCCGGGACCATA
CA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 951 bp | 316 aa | 57 | 1007 | + | No |
Chemistry : DDE
ORF sequence :
MGQVLHGSATTTEAVRRAIQLREESVRSLAKRYGVSPTTVQKWRKRTSTADARMGPKEPRSTVLTVEEEAIVVAFRRHTLLPLDDCLYGLQPTIPHLTRS
SLYRCLERHGISRLPEIDGDKPKKQRFATYAIGYVHIDIAEVSTAEGKLRLFVAIDRTSKFAFVRLVESAGKMEAAQFLRDFIDAVPYRIHTVLTDNGVQ
FTSRRQDIYDSQHIFDRVCDEHDIEHRLTKVNHPWTNGQVERMNRTIKDATVKRYHYDSHDQLRTHLHLFVDAYNHARRLKTLRGLTPTEFILNAWTKEP
NRFRIDPSHLIPGPYT
SLYRCLERHGISRLPEIDGDKPKKQRFATYAIGYVHIDIAEVSTAEGKLRLFVAIDRTSKFAFVRLVESAGKMEAAQFLRDFIDAVPYRIHTVLTDNGVQ
FTSRRQDIYDSQHIFDRVCDEHDIEHRLTKVNHPWTNGQVERMNRTIKDATVKRYHYDSHDQLRTHLHLFVDAYNHARRLKTLRGLTPTEFILNAWTKEP
NRFRIDPSHLIPGPYT
Blast result :
Comments
ISMdi11 is 81% aa similar to ISNe2.
There is no stop codon at the end of the element, the transposase use the first STOP codon after the end of the IRR. The transposase in the database is complete.
There is no stop codon at the end of the element, the transposase use the first STOP codon after the end of the IRR. The transposase in the database is complete.
References
1] Stéphane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS ONE Submitted. (2009)