ISMdi4
- Family IS481
- Group
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| ND | Methylobacterium dichloromethanicum | Methylobacterium dichloromethanicum DM4 Methylobacterium extorquens PA1 |
DNA section
IS Length : 1053 bp
Ends
IR Length : 17/26
IRL : TGTTTGGTCCCGCTGTGTGGTGCTACAACTGATGCCGCATGGAGGGACGC
IRR : TGTACGGTCCCAGGAGGTGGTGTGACGGATCGAGCCTGAAGCGTTCGGGC
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGATCCTAGA | TCTAGTAACC | 0 | |
| CCGTACTAGA | TCTAGAGCGG | 0 | |
| AGATCCTAGA | TCTAGTCTAG | 0 | |
| TCGCTCTAGA | TCTAGCTAGT | 0 |
DNA sequence
TGTTTGGTCCCGCTGTGTGGTGCTACAACTGATGCCGCATGGAGGGACGCGCTATGGGACAGGTTCTCCACGGCAGCGCCACGACGACTGCGACGGTCCG
TCGGGCAATCCAAGCTCGTCAAGAGAGCGTGAGGGCCGCTGCCAAGCGCTACGGCATCAGCCCCACGACGGTGCAGAAGTGGCGCTCACGCCAGACCAGC
ACGGATGCACGGATGGGGCCGAAGGAGCCCCGCTCCAGCGTGCTCAGCCTGGAGGACGAGGCGGTCATCGTCGCCTTCCGGCGGCACACCCTGCTGCCGC
TCGACGACTGCCTCTACGCGCTGCAGCCGAGCCTGCCGCATCTGACCCGCTCGTCACTCCACCGCTGCCTGCAGCGTCACGGCATCAGCCGCCTGCCGGA
TGTGGACGGCGACAAGCCGAAGCGGTCGCGCTTCAAGGCCTACCCGATCGGCTACTTTCACATCGACATCGCACAGGTCAGCACGGAACAGGGCAAGCTG
CACCTGTTCGTGGCCATCGATCGCACGAGCAAGTTCGCATTCGTTCAACTGCACGGACGAGCAACGCAACGGATCGCGGCAGCCTTTCTACACGATCTGG
TCGCCGCCGTGCCGTACACGATCCACACGGTGCTGACGGATAATGGCATCCAGTTCACCGACAACCATCCGGTGGACGAGGAGGCCGAGGCTAAGGCGGC
AGCTTATTGGGCCGAACGCGACGAGCCACGCATCTACCGCTGGCACTCGTTCGAGTGGGCCTGCGAGCAGACCAAGATCGAGCATCGCCTGACCAAGCCG
CGCTGCCCGTGGACGAATGGCCAAGTCGAGCGGATGAACCGCACCATCAAGGACGCCACGGTCAAGCGCTATCATTACGCCAGCCACGACGAGCTGCGCC
ACCACCTGCAGCTGTTCGTGGATGCCTACAACTACGGCCGCCGGCTCAAGACCCTGCGCGGCCTCACGCCCTACGAGTTCATCTGCCAGACCTGGACCAA
CGAGCCCGAACGCTTCAGGCTCGATCCGTCACACCACCTCCTGGGACCGTACA
TCGGGCAATCCAAGCTCGTCAAGAGAGCGTGAGGGCCGCTGCCAAGCGCTACGGCATCAGCCCCACGACGGTGCAGAAGTGGCGCTCACGCCAGACCAGC
ACGGATGCACGGATGGGGCCGAAGGAGCCCCGCTCCAGCGTGCTCAGCCTGGAGGACGAGGCGGTCATCGTCGCCTTCCGGCGGCACACCCTGCTGCCGC
TCGACGACTGCCTCTACGCGCTGCAGCCGAGCCTGCCGCATCTGACCCGCTCGTCACTCCACCGCTGCCTGCAGCGTCACGGCATCAGCCGCCTGCCGGA
TGTGGACGGCGACAAGCCGAAGCGGTCGCGCTTCAAGGCCTACCCGATCGGCTACTTTCACATCGACATCGCACAGGTCAGCACGGAACAGGGCAAGCTG
CACCTGTTCGTGGCCATCGATCGCACGAGCAAGTTCGCATTCGTTCAACTGCACGGACGAGCAACGCAACGGATCGCGGCAGCCTTTCTACACGATCTGG
TCGCCGCCGTGCCGTACACGATCCACACGGTGCTGACGGATAATGGCATCCAGTTCACCGACAACCATCCGGTGGACGAGGAGGCCGAGGCTAAGGCGGC
AGCTTATTGGGCCGAACGCGACGAGCCACGCATCTACCGCTGGCACTCGTTCGAGTGGGCCTGCGAGCAGACCAAGATCGAGCATCGCCTGACCAAGCCG
CGCTGCCCGTGGACGAATGGCCAAGTCGAGCGGATGAACCGCACCATCAAGGACGCCACGGTCAAGCGCTATCATTACGCCAGCCACGACGAGCTGCGCC
ACCACCTGCAGCTGTTCGTGGATGCCTACAACTACGGCCGCCGGCTCAAGACCCTGCGCGGCCTCACGCCCTACGAGTTCATCTGCCAGACCTGGACCAA
CGAGCCCGAACGCTTCAGGCTCGATCCGTCACACCACCTCCTGGGACCGTACA
Protein section
ORF number : 1
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 1020 bp | 339 aa | 39 | 1058 | + | No |
Chemistry : DDE
ORF sequence :
MEGRAMGQVLHGSATTTATVRRAIQARQESVRAAAKRYGISPTTVQKWRSRQTSTDARMGPKEPRSSVLSLEDEAVIVAFRRHTLLPLDDCLYALQPSLP
HLTRSSLHRCLQRHGISRLPDVDGDKPKRSRFKAYPIGYFHIDIAQVSTEQGKLHLFVAIDRTSKFAFVQLHGRATQRIAAAFLHDLVAAVPYTIHTVLT
DNGIQFTDNHPVDEEAEAKAAAYWAERDEPRIYRWHSFEWACEQTKIEHRLTKPRCPWTNGQVERMNRTIKDATVKRYHYASHDELRHHLQLFVDAYNYG
RRLKTLRGLTPYEFICQTWTNEPERFRLDPSHHLLGPYI
HLTRSSLHRCLQRHGISRLPDVDGDKPKRSRFKAYPIGYFHIDIAQVSTEQGKLHLFVAIDRTSKFAFVQLHGRATQRIAAAFLHDLVAAVPYTIHTVLT
DNGIQFTDNHPVDEEAEAKAAAYWAERDEPRIYRWHSFEWACEQTKIEHRLTKPRCPWTNGQVERMNRTIKDATVKRYHYASHDELRHHLQLFVDAYNYG
RRLKTLRGLTPYEFICQTWTNEPERFRLDPSHHLLGPYI
Blast result :
Comments
ISMdi4 is 78% aa similar to ISGdi9.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR.
The transposase in the database is complete.
There is no stop codon at the end of the element, the transposase use the first Stop codon after the end of the IRR.
The transposase in the database is complete.
References
1] Stéphane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS ONE Submitted. (2009)