ISMex13

  • Family IS21
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
DNA section
IS Length : 2359 bp

Ends


IR Length : 38/50

IRL : TGTCAGCGGGCGTTGAATACTCCCTGATTGTGGGCATCGAAAATTCCCTG
IRR : TGTCAACGGGCACCGAAGTCTCCGCAATTGTGGGCTTTCAAAATTCCCTG

Insertion site


Left flankDirect repeatRight flankDR Length
GCAGAACTTCGCAGGCACGCTTGCG5
TCCGCCAATACCTGCTGTGCACCCG5
TAGTGGGTCAGGATCCGATGCAGCAC6
GCGGCGAGTGACCTTGCCCCCTGGC5

DNA sequence

TGTCAGCGGGCGTTGAATACTCCCTGATTGTGGGCATCGAAAATTCCCTGGTCGGTGCCCTGGCCCGTAGGGTCGCGACGCCCCGCGCCCTCTCGGGCTT
TTCCATCCTCAGCCACCATGCCTGCCGATACCGATCGGCTGGGAGCGACGAGGATGGTTCTGCTGGGAGAACTCGTCATGATCTTGGACCTGCACCGACA
GGGCCTGTCCGTCTCCGCCATCGCCCGCCGGACCGGCCGCGATCCGAAGACGATCCGCAAGTACATCGAGCGCGGCCTCGAGCCGCCGGCCTACGGCCCG
CGTCAGCCCGGCCGCCCGAGCAAGCTCGCGCCCTATCTCGATTATCTGCGCGAGCGGATCACCGCCTTCCCCGACCTGAGTGCCGTGCGCCTGACCCGCG
AGTTGCGCGAGCGCGGCTACACCGGTGCCTACACCGCGGTGAAGCGGTTCGCCGCCGCGATCCGGCCGCCCGAGGCCAAGCCCTACGAGGTCCGCTTCGA
GACCCCGGCCGGCCAGCAGGCGCAGGTCGACTTCGCCCGCTTCCTCGTCACCTTCACGGATGCGCCGGACACGACCTGCATCGTCTGGCTGTTCTCGCTG
GTGCTCGGCCATTCCCGGCACATCGAGGCGCGCTTCGTCCTGCATCAGGACCTGCAAACGCTGCTGCGCTGTCACATGCAGGCCTTCACCGCGATCGGCG
GCGTGCCGATCGAGATCCTCTACGATCGCATGAAGACGGCGGTCACCGGCGAGGATGCGGACGGCCACATCGTCTACAACCGATCCCTGCTGGCACTCGC
CCAGCACTACGGATTCCTGCCGCGCGCCTGCCGCCCGTACCGGGCCAAGACCAAGGGGAAGGTCGAGAGACCGTTCTCCTACATCCGCCAGGACTTCTTC
CTCGCACGTTCCTTCCGCGACCTCGACGACCTCAACCGCCAGCTTCGGAGCTGGCTCGATACCGTCGCCAACGTCCGCTTGCACGGCACCACGCAGCGGA
TCGTCTCGGAAGCCTTCGCCGCCGAGCGGCCCGAGTTGCAGCCCTTGCCGGCTCTGCCCTTCGACGCTCTGCTCACGCTGGAGCGGCGCGTCAGCCACGA
TGGCCTCGTCTCGATCGGTGGCAACTATTACAGCGTACCGGATCGGACCCGGCGCGTCGTCGAGGTGCATCAGTTGCCCGACACGATCCGCATCCTCGAT
GGTGGCCGGCTCGTCGCGAGCCATCCGATCCTGGAGGGACGACGGCAGTACCGCATCGACCCCGACCATCGGCAAGGCACGGCCGCTCGGGCCATGCGCC
GCGGCCATCCCGACGGTCTGCCGATCGGCCGCCATGGCGATCACGTCGCCCGGCGCTCGCTGGCTGTCTACCAGGCAGTCGGCGAACGGCTCGCCGGCGG
GATCGGAGGCCAGCGATGAGCCGCGCCGCCCCCTGTGTCGCCACGACCCTCGACAGCATCAAGCGCAGCTTGGTCGGCCTGCGCATGCCGCGCGCCCTGG
AGGTGCTCGACGCGACGGTCCGGCGCATCGAGCAGGGCGAGATCGACGGCTTGGCCGCCCTCGACGTGATCCTGACCGAGGAACTGACGCTGCGCGAGAA
CCGCCGCGTGAAGACCGCCCTGCTGGTCGCGCGCCTGACCACGATCAAGACGCTGTCCGGGTTCGACTTTGCCTTCCAGCCCTCGCTCGACCGCGAGCGC
GTCCTGGCGCTGGCGGAACTGACCTTCATCGACCGGGCCGAGGTCGTCCATCTGCTCGGACCACCCGGCACCGGCAAGAGCCATCTGGCGATCGCGCTCG
CCGTCGAGGCGGTCAAGGCCGGGCGCAGCGTCGTGTTCTCGACGCTGGCCGACCTCGTGACCTCGCTGGCCAAGGCCGAGCGCGACGGCTCCCTGCGCGA
GCGCATCCGCTATCTCTGCCGGGCCTCGCTGCTGGTCGTGGACGAGATCGGCTACCTCCCCGTCGTCCCCGGTGGCGGCAACCTGTTCTTCCAACTCGTC
AACGCGCGCTACGAGCGCGGGGCGATGATCCTGACTTCGAACCGCGGCTTCGCCGAGTGGGGCGAAGTGTTCGGTGATCCGGTCGTGGCGACAGCCCTGC
TCGACCGACTCCTCCACCATGCCGTGGTGATCCAGATCGAGGGGGCGAGCTACCGCCTGCGCCAGCACGCCGACCTCGTCCCCGAGCACGTCCGCTCCAA
GGCCCTGATCGCTCCGCCGCCCGCACCCAGGCGTCGCGGTCGTCCACCCGGAAAGGCTGCCTCCGATCACACGGCCGGCTGATCACCGATCCGCACCGAA
CCCGCCGGCCAGGGAATTTTGAAAGCCCACAATTGCGGAGACTTCGGTGCCCGTTGACA
Protein section
ORF number : 2

 

ORF 1
LengthBeginEndStrandFusion ORF
1392 bp463 aa281419+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LWASKIPWSVPWPVGSRRPAPSRAFPSSATMPADTDRLGATRMVLLGELVMILDLHRQGLSVSAIARRTGRDPKTIRKYIERGLEPPAYGPRQPGRPSKL
APYLDYLRERITAFPDLSAVRLTRELRERGYTGAYTAVKRFAAAIRPPEAKPYEVRFETPAGQQAQVDFARFLVTFTDAPDTTCIVWLFSLVLGHSRHIE
ARFVLHQDLQTLLRCHMQAFTAIGGVPIEILYDRMKTAVTGEDADGHIVYNRSLLALAQHYGFLPRACRPYRAKTKGKVERPFSYIRQDFFLARSFRDLD
DLNRQLRSWLDTVANVRLHGTTQRIVSEAFAAERPELQPLPALPFDALLTLERRVSHDGLVSIGGNYYSVPDRTRRVVEVHQLPDTIRILDGGRLVASHP
ILEGRRQYRIDPDHRQGTAARAMRRGHPDGLPIGRHGDHVARRSLAVYQAVGERLAGGIGGQR

 

Blast result :
ORF 2
LengthBeginEndStrandFusion ORF
867 bp288 aa14162282+No
ORF function : Accessory Gene
AG : IS21 helper

ORF sequence :

MSRAAPCVATTLDSIKRSLVGLRMPRALEVLDATVRRIEQGEIDGLAALDVILTEELTLRENRRVKTALLVARLTTIKTLSGFDFAFQPSLDRERVLALA
ELTFIDRAEVVHLLGPPGTGKSHLAIALAVEAVKAGRSVVFSTLADLVTSLAKAERDGSLRERIRYLCRASLLVVDEIGYLPVVPGGGNLFFQLVNARYE
RGAMILTSNRGFAEWGEVFGDPVVATALLDRLLHHAVVIQIEGASYRLRQHADLVPEHVRSKALIAPPPAPRRRGRPPGKAASDHTAG

 

Blast result :
Comments
ISMex13 is 72% (istA, the transposase) aa similar to ISBcen28 and 84% (istB, the helper of transposition) to ISRe13.
References
1] Stephane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. (2009) PLoS ONE Submitted.