ISMex4

  • Family IS1380
  • Group
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
Y Methylobacterium extorquens
Methylobacterium extorquens AM1
DNA section
IS Length : 1615 bp

Ends


IR Length : 16/19

IRL : CCCGTCTTATTCACGAGGGCAGTGGATCTGTGGTGGCGAGCGTAGCGTAA
IRR : CCCGCTTTATTCACCAGGGTTTGGCTGGCGACGTGTTTTGAGCTGAAGGC

Insertion site


Left flankDirect repeatRight flankDR Length
CCCGTGGCGTCTAGAGTCGCTCTC4
GAGGCCGGAGCTAGCTTCGACGCA4
ATACGACATCCTAGTCCCACGCGG4
GCTTTCCTAGCTAAGGGCCG0
GGACGGGCTCCTAGAACCGCATTC4
GGACCAAAGCGTAGTGTTCGCGGA4
CTCTTGTACCTTAGTCTACGCGCG4
GCTCATCTAGCTAAGAGGCG0

DNA sequence

CCCGTCTTATTCACGAGGGCAGTGGATCTGTGGTGGCGAGCGTAGCGTAAAGCATTGATGTGGCTTAGGGATTGGGTGCTGACGCCAAGTCCTCAGACCA
GATCGAGCCCGCCACGCCCGCCATGTCCGATCCTACGTGTCTGCCGTTCGCGTTTCCATCGGTTCGAGGCAAGAAGCTCACAGCTGCTTTCGACGGCGGA
CGCCTGACCTCGGACGGGGGCGTCCTGTTGCTCGCTCAGGCTGCCCGGCGGATGCAGATCGGTGAGAAGCTCGCCGCGGTGATCCCCGACCGGCGTGACC
CGAGCCGGATCGTGCATCCTCTGCCCGAGATCCTGCTGGCGCGCATCCTGGCCATCGCCTGCGGGTACGAGGATGCCGACGACCTCGATCACCTGCGCGT
CGATCCCGCCTTCAAGCTCGCCTGTGGTCGCCTACCCGACAGCGGACGCGACTTGATGAGCCAGCCCACCGTCTCGCGGCTGGAGAACACGCCGGGCCTG
CGTGACCTGATCCGGCTCGGACGGGTGCTGGTCGACCTCTACTGCGCCAGCTACGCCGCGCCACCGGCGGCTGTCACGCTGGATATCGACGACACCTGCG
ACGTGGTCCATGGCCAGCAGCAACTGTCGCTGTTCAACGCCCACCACGACGAACGCTGCTTTCTCCCCATCCACGTCTACGACACCGCCACCTCGCGCCC
GGTGGCGATGATCCTGCGGCCGGGCAAGACGCCTTCGGGTCGCGAAGTCCGGGGCCATCTGCGTCGTCTGGTCCGGGCGATCCGCCGACACTGGCCGACG
ACCGCGATCACGATCCGTGGCGACGGCCATTACGGGCGGCCCGAAGCGATGGACTGGTGCGAGGACAACGACGTCGCTTACGTCTTCGGCCTGACCGGCA
CCAAGTCGCTCACCGCCAAGGTCGAGCCGACCGCCGATCACATCCGGGTCGAGCGGGCCCTCAGCAACACAGATGCCGTGCGCGGCTTTGCTGAGACCAC
GCACGCAGCCAAATCCTGGCGGCAGCACCGCCGCGTCGCCGCCCGCATCGAGGCGACCCGCCTTGGCCTCGACATCCGCTACGTGGTCACGAACATCGCC
ACCGGTACGCCCGAATGGCTCTACGCCGAGCTGTACTGCGCCCGCGGACAGGCCGAGAACCTGATCAAGCTCCATAAGGGCCAGCTCGCTTCCGACCGCA
CCTCCTGCCGGCATCCGGCCGCCAACCAGATGCGGCTCGTGCTGCACACCGCCGCCTATTGGCTGATGCTGACCGTGCGCGACGCCATCCCGGCTCCCCA
TCGCCTGGCCAAGGCCGAGTTCGCGACGATCCGCCTGCGCCTGCTCAAGCTTGGGGCTCGCATCCACGAGACCACTGCCCGTGTTCGTCTCGCCTTCGCC
GCCGCCTGTCCCGAGGCCGACTTGCTGCGCCATATCGCGGGCGCACTCGCCCCAGCGCCAGCCTGAACCACGGGGCGTGATGCCCCATCGACACCGCTCC
CGTCCCTCCAGCGCCGACAGCACAACCAGATCCATACGCGTCAGGACAGACGCACGATCCCGCTCGCCTTCAGCTCAAAACACGTCGCCAGCCAAACCCT
GGTGAATAAAGCGGG
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1395 bp464 aa1501544+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

LGADAKSSDQIEPATPAMSDPTCLPFAFPSVRGKKLTAAFDGGRLTSDGGVLLLAQAARRMQIGEKLAAVIPDRRDPSRIVHPLPEILLARILAIACGYE
DADDLDHLRVDPAFKLACGRLPDSGRDLMSQPTVSRLENTPGLRDLIRLGRVLVDLYCASYAAPPAAVTLDIDDTCDVVHGQQQLSLFNAHHDERCFLPI
HVYDTATSRPVAMILRPGKTPSGREVRGHLRRLVRAIRRHWPTTAITIRGDGHYGRPEAMDWCEDNDVAYVFGLTGTKSLTAKVEPTADHIRVERALSNT
DAVRGFAETTHAAKSWRQHRRVAARIEATRLGLDIRYVVTNIATGTPEWLYAELYCARGQAENLIKLHKGQLASDRTSCRHPAANQMRLVLHTAAYWLML
TVRDAIPAPHRLAKAEFATIRLRLLKLGARIHETTARVRLAFAAACPEADLLRHIAGALAPAPA

 

Blast result :
Comments
In independently evolved populations founded with AM1, multiple ISMex4 transposition events were observed. ISMex4 inserted into upstream region of icuAB, a novel cobalt transporter system, and increased the expression level.
ISMex4 is 76% aa similar to ISSp1.
References
1] Stéphane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS ONE Submitted.
2] Ming-Chun Lee (2009) Direct submission.