ISMex6

  • Family IS1202
  • Group ISAba32
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
ND Methylobacterium extorquens
Methylobacterium extorquens AM1
DNA section
IS Length : 1535 bp

Ends


IR Length : 9/16

IRL : TGTAGGCGCAAAATTGAGATGACACCCCCTGGCAAAGTTGAAACGGCACC
IRR : TGTCAACGCTAATCAGAAATGGCACCGCTTCCGCAAAGTAGACATGACAC

Insertion site


Left flankDirect repeatRight flankDR Length
TATGCGAACGACGACATGCTGGTTAG6
GCAGCCCATGAAAGCCGTTCGCATA5

DNA sequence

TGTAGGCGCAAAATTGAGATGACACCCCCTGGCAAAGTTGAAACGGCACCCCCTCTCCCTCTGATGGATGTGGAGGGTGCGCGCGATGGGGTTGAAGTCC
ATGAGCGGCAAGGAATTTTCGCGCCTCGATGTTCTGCTCGACGTCGAGGCTGGGCGCCTTGCTATCGATGATGCCTGCGATCTGATGGGGCTGCGGCGGC
GGCAGGTTTTTCGCCTGCTCGCGGCATTTCGGAAGCAAGGCGCAGCCGGTCTGATCTCGAAGCGGCGCGGTCGGCCGAGCAACAATCGCCTGCCCGATGA
AGTGCGTCAGTTGGCCATGAGCATTGTCCGAGAGCGATATTCGGACTTTGGCCCTACCCTCGCCTGTGAGAAGCTCGCCAACCTACATGATTGTCCAGTC
TCTCGTGAAACGTTGCGCAAGTGGATGATGGAAGACGGTCTGTGGTTGGATCGTAGGCGCCGCCTCCCTTCCGTACATCAGCCCCGGCTTCGTCGCGACC
GTGTTGGCGAATTGATTCAGATCGATGGCTCGAAGCATTACTGGTTTGAGAGTCGTGGGCCGGAATGCACGTTGCTTGCGTTCATCGACGACGCAACGAG
TCGGATCATGCACGCTGCCTTTGTGCATTCGGAGTCTGCATTCGACTACCTCCGAGAAACACGGGCTTACCTGTTGAAGCATGGGCGCCCTACTGCATTC
TATTCCGACAAGCACGCGGTCTTTCGTGTCAGCAAGCGGGATGCCGCTGGCGGGACAGGCATGACCCAATTTGGACGCGCCTTGGACGAACTCAACATCG
ACATCATGTGCGCGAACACGCCCTCAGCGAAGGGTCGTATTGAGAGAGCATTCGGCACCTTGCAGGATCGTTTGATCAAGGAGATGCGACTTGCAGAAAT
TTGTTCGATGGAAGATGGCAACAAGTTTCTGGTCGGATTTATAGCCGATCACAACGCTCGTTTCGGCAAGCCGCCTTTCGATGAGCGAGACGCCCATCGA
GCGCTTCAGGGCGATATCATTCTGGACGATGTGTTTGCTTGGAAGGAGGAGAGAACAGTCACGAACAGCCTTACGCTCCAGTACGACAAGGTCACATTCA
TCTTGGAGCCGACTGCTGTCACGCGCCCCCTTGCTCGTAAGCGTGTGCAGGTGATCGATTATCCGGACGGACGCTTCGCGATCCGACATCAAGGCTTGGA
TTTACCGTTTCGCACCTTCGATCGGATACGCCATGTCGATCAGGCGGCGATCGTGGAGAACAAGCGCCTGGGTGCGGTCCTGGCGTTCATTGCCGAGCGG
CAGCAGGAGCGGGATCAAAACCGTTCACAGAAGGCGCCTCGTCGGCAAGGGCAGGTGGACCGGCACATGTTCAAGAGCGGGTGAGGGCACCCGGCCGTGT
CGATCTGGATCCGGTGACACCGCAGCTTCGCCACCCCGACCCGCTTCGCTGCGGTGTCCCCTCCCCTCTGTGCCTTCTAAGAGGGGTGTCATGTCTACTT
TGCGGAAGCGGTGCCATTTCTGATTAGCGTTGACA
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
1299 bp432 aa861384+No
ORF function : Transposase
Chemistry : DDE

ORF sequence :

MGLKSMSGKEFSRLDVLLDVEAGRLAIDDACDLMGLRRRQVFRLLAAFRKQGAAGLISKRRGRPSNNRLPDEVRQLAMSIVRERYSDFGPTLACEKLANL
HDCPVSRETLRKWMMEDGLWLDRRRRLPSVHQPRLRRDRVGELIQIDGSKHYWFESRGPECTLLAFIDDATSRIMHAAFVHSESAFDYLRETRAYLLKHG
RPTAFYSDKHAVFRVSKRDAAGGTGMTQFGRALDELNIDIMCANTPSAKGRIERAFGTLQDRLIKEMRLAEICSMEDGNKFLVGFIADHNARFGKPPFDE
RDAHRALQGDIILDDVFAWKEERTVTNSLTLQYDKVTFILEPTAVTRPLARKRVQVIDYPDGRFAIRHQGLDLPFRTFDRIRHVDQAAIVENKRLGAVLA
FIAERQQERDQNRSQKAPRRQGQVDRHMFKSG

 

Blast result :
Comments
ISMex6 is 56% aa similar to ISCARN112.
References
1] Stéphane Vuilleumier et.al. Methylobacterium genome sequences: a reference blueprint to investigate microbial metabolism of C1 compounds from natural and industrial sources. PLoS ONE Submitted.
2] Ming-Chun Lee (2009) Direct submission.