ISfinder

ISMae8

Family ISAs1
Group

MGE type ISRelated element(s) :
Isoform Synonym(s) ISMae35

Accession number	Transposition	Origin	Host
NC_010296	ND	Microcystis aeruginosa	Microcystis aeruginosa NIES-843

DNA section

IS Length : 1418 bp

Ends

IR Length : 13/15

IRL : TAGGGCTGTTTCATTCTCCCATCAGAATATCAAAAGTAAAAGCGATCAAT
IRR : TAGGGTTGATTCATTTAAATTAAGTACAGCGGATTTTGAGTCAATAAATT

Insertion site

Left flank	Direct repeat	Right flank	DR Length
AGGTTCGATCCC	CCCTGCCCCC	CCTTGATAAGGTAG	10

DNA sequence

TAGGGCTGTTTCATTCTCCCATCAGAATATCAAAAGTAAAAGCGATCAATATCAGGTAAAATGATAAGTGACCGCCAACCTTTTAAATATATGTTGAGCT
TAATAGAAAAACTGAAACAAGTCAAGGACTTTCGGAAAGATAAAGGAAAAAGACACCCTTTATGGATAGTATTAGTAGTAATAATACTGGGAACAATGCT
AGGATACTCAGGTTATAGAGAACTAGGAGAGTTTGCTAAAAATAATCGGCACAGGCTCAGTAAAGAATTTAACATAATTCCAGAAAGAGTCCCATCCTAT
TCAACAATTAGAAGGGTAATGATGGGAGTTGACTGGCAGAGTTTGTTAAAAATGTTTAATGAATGGGCATTAGAAGAATATGGACAAAGAGATGATATAA
ATTGGCTAGGTATGGATGGAAAAAGTCTCAAAAACACCCTAAAGAATCCTAATAATGAACAACAAAATTTTATCATGTTTGTCTCATTGTTTAGTCAAGA
AAGTGGATTAGTATTACACTTAAAAAGAATCGAAAACAAAAAAGGGTCTGAAATCGACGAAGGGCAAGCTATAATTGAGGATTGCTCTCTCCAAAATAAA
GTTTTTACTGGCGATGCTTTACACTGTCAGAAAAAAACAATCAGCTTAATAGCCAAGAGTAAAAATGACTATGTTATCACCGTTAAAGGAAATCAGAAAA
ATCTTTATAAGCGAATACAAGACCTGAGTAATTCCTCAAAGCCAGAAAGTTGCTTTCTTGAACAAGATAATAGTCATGGACGAAAAATATCAAGAAAAAT
AGAAGTTTTTAAAGTGAGAAAAAATGAAAGACAAGGGTTTGAAAATCTGCGAAGAGTTATTAAAGTAGAAAGAAAGGGTAGTCGCGGGGATAAAACCTAT
GAAGAAACAGCTTACTATATCAGTAGCCTAACCGAATCCGCTCAAGTATTTGCTAAAATTATTCGAGGACATTGGAAAATAGAAAATCAGTTACATTGGG
TAAAAGATGTAATTTTTGAGGAAGATAAAAGCGAGATAAGTGATTTTCAAGCGGCCAGCAATTGGTCAATTCTCACAACTATAGGATTGAATCTTTTCAG
AGGTTTGGGTTTTCTCTCAATAACAGAGGGACAGAGGTGGTTAGCTGAACGTTGGGAAAAACTGATAGTTTTATCGACGTAAGAAGCAGAAAAATTAGCT
AAATTAACAGGATGTACTCTCAGACAATTTCAAGAGAGATAGGCTTTTAGTGGCTTGGAAACAGGCTTTATCAATTTATTCATAATCAATATTGGCAGAG
GATTAAAGATTAGTTATTGTCACTAACTCTTTTGATTAGAAAAAGATAAACTTGAGAATTTTCATTCAAATTTATTGACTCAAAATCCGCTGTACTTAAT
TTAAATGAATCAACCCTA

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1092 bp	363 aa	91	1182	+	No

ORF function : Transposase
Chemistry : Unknow

ORF sequence :

MLSLIEKLKQVKDFRKDKGKRHPLWIVLVVIILGTMLGYSGYRELGEFAKNNRHRLSKEFNIIPERVPSYSTIRRVMMGVDWQSLLKMFNEWALEEYGQR
DDINWLGMDGKSLKNTLKNPNNEQQNFIMFVSLFSQESGLVLHLKRIENKKGSEIDEGQAIIEDCSLQNKVFTGDALHCQKKTISLIAKSKNDYVITVKG
NQKNLYKRIQDLSNSSKPESCFLEQDNSHGRKISRKIEVFKVRKNERQGFENLRRVIKVERKGSRGDKTYEETAYYISSLTESAQVFAKIIRGHWKIENQ
LHWVKDVIFEEDKSEISDFQAASNWSILTTIGLNLFRGLGFLSITEGQRWLAERWEKLIVLST

Blast result :

Comments

ISMae8 is 73% aa similar to ISPlr1.
The ends are not CAGGG as in other ISAs1 family member but TAGGG, and it is the same in all copies in this genome.

References

1] ISfinder annotation (2009)
2] Kaneko,T., Nakajima,N., Okamoto,S., Suzuki,I., Tanabe,Y., Tamaoki,M., Nakamura,Y., Kasai,F., Watanabe,A., Kawashima,K., Kishida,Y., Ono,A., Shimizu,Y., Takahashi,C., Minami,C., Fujishiro,T., Kohara,M., Katoh,M., Nakazaki,N., Nakayama,S., Yamada,M., Tabata,S. and Watanabe,M.M. (2007) DNA Res. 14 (6), 247-256