ISOih2

  • Family IS200/IS605
  • Group IS200
MGE type ISRelated element(s) :
Isoform Synonym(s)
Accession numberTranspositionOriginHost
NC_004193 ND Oceanobacillus iheyensis
Oceanobacillus iheyensis HTE831
DNA section
IS Length : 739 bp

Ends


Left end : TATGGTCTTAGAAAACCCCTGGCTATGCCAGGGGTTCCAGAAAGCTTCTAGCGTGGGGTAAAAAAAAGCCTCTCTTCATGGTAAGGTAAGGTTGGTTTCC II struct. : Yes
Right end : TGCAGAAGGGGAAACCATTCAGTGGGCCTTTTAGGCCGAGGCCGGCAACAGAGGCTTCCAGCCGCAGAGCAAACCACCAGTTCACACTGGTGGTTTTGAT II struct. : Yes

Insertion site


Left flankLE cleavage siteRight flankRE cleavage site
TTCGCTGCTTTTTTGTTTTGGTCTTAGATTAAATATtgat
ACTTTGCCCACTATTTTTCTTTGAAAGAAAGCTCGAtgat

DNA sequence

TATGGTCTTAGAAAACCCCTGGCTATGCCAGGGGTTCCAGAAAGCTTCTAGCGTGGGGTAAAAAAAAGCCTCTCTTCATGGTAAGGTAAGGTTGGTTTCC
CGACCACCAAATCTCACCATAGAAGGAGGCGTGTCCAATGAAGGACAAGAATAGTTTAGCACACACGCAATGGAGATGTAAGTATCATTTAGTTTTTGCC
CCAAAATATAGAAGACAAATTATCTATGGAAAATATAAAAAGAGTATAGGAGAAATTATTAGAGAACTCTGCGAAAGAAAAGATGTTGAAATACACGAAG
CAAATGCATGCAGAGACCACGTCCATATGTTGGTGAGTATACCACCAAAACTGAGTGTATCTCAATTTATGGGTTACTTAAAAGGAAAAAGTAGTTTAAT
GATCTTTGACCGTCACGCTAACTTAAAGTATAGATATGGCAATCGAAAGTTCTGGTGTCGAGGCTACTTTGTAGATACGGTGGGAAGGAATAAAAATCAA
ATCCAAGAATATATACGAAATCAGCTTAAAGAGGATTATATGAGTGATCAGTTAACTTTATTTGAAGAATTTGATCCGTTCACCGGAAAGAAAAATAAAA
AGAAATAAGAGATTCTTTTAGAATCAGTGAGTGAGTGTGGTGCAGAAGGGGAAACCATTCAGTGGGCCTTTTAGGCCGAGGCCGGCAACAGAGGCTTCCA
GCCGCAGAGCAAACCACCAGTTCACACTGGTGGTTTTGAT
Protein section
ORF number : 1

 

ORF 1
LengthBeginEndStrandFusion ORF
471 bp156 aa138608+No
ORF function : Transposase
Chemistry : Y1

ORF sequence :

MKDKNSLAHTQWRCKYHLVFAPKYRRQIIYGKYKKSIGEIIRELCERKDVEIHEANACRDHVHMLVSIPPKLSVSQFMGYLKGKSSLMIFDRHANLKYRY
GNRKFWCRGYFVDTVGRNKNQIQEYIRNQLKEDYMSDQLTLFEEFDPFTGKKNKKK

 

Blast result :
Comments
ISOih2 is 95% aa similar to IS657.
References
1] ISfinder annotation (2008)
2] Takami,H., Takaki,Y. and Uchiyama,I.(2002) Nucleic Acids Res. 30 (18), 3927-3935