ISfinder

ISMasp5

Family IS66
Group ISBst12

MGE type ISRelated element(s) :
Isoform Synonym(s)

Accession number	Transposition	Origin	Host
NC_008576	ND	Magnetococcus sp.	Magnetococcus sp. MC-1

DNA section

IS Length : 1967 bp

Ends

IR Length : 19

IRL : GTAACCGTTCAGAGACCCCTCGTTCAGTGAGGATGTTCACCGTCTGAGAA
IRR : GTAACCGTTCAGAGACCCCGATATCAAGCGGCTACTGGAGCCGGTAACGG

Insertion site

Left flank	Direct repeat	Right flank	DR Length
GTTTTTTTGC	CACCTACC	CCCCTCTAGA	8
GGCCGCGTAA	GATGAATC	AGACGTCCAC	8

DNA sequence

GTAACCGTTCAGAGACCCCTCGTTCAGTGAGGATGTTCACCGTCTGAGAAGGAATTAACTGCTGGGGCTGGCACCAATTTTGTTTGATCTTGGCAAAGAA
TATCCTTGACACGATTTTCGTCGAAAACGTGGAATTTCAAAAGGAGAGTGCGATCCGATCTATTTTGCCTATCAAAGCCTTGAGGGGGAATTGTGGCGGG
TTTTCAGCTGTGGGCCCACCCCGGAGACGAGCGTATTTTTACACTGGCTCCAATGGGGCGCAGAAGCTCAATAATCTCTTCCTGGAAACTCGTGTTATCA
TGTTCATGTTGATTCATTCACCGGCGATGATATGGCGCACGAACTATGAAGCTTTCGGATCACGACCTGCTCCAGATTGATGAGGAATATCTCTCCTCGC
TATCGTCTGCAGAATTGCTGGCCGTCAGCCGGAAAATGTTGGAGGATCTTAAGGAATCTCGTGAGCGTTTGAACCGGACACCTGATAACAGCTCCCAGCC
GCCCAGCAGTCGTCCGGCGTACCTTGGAATTCCAGTTGAGGAGGATGAAGCGCATCTGGATGAGGATGAAGATGACGCTTCGCCGGATAAAAAGAAGGGG
GCCGATGATGAGGGTGGAGATGATTCCCCAGGGCCTTCCGGTAATTCTACTCCCCCTGCTCCAGGAAAGGGCAAGCCGGATTCTTCAAGCGAGAAGCCGA
AAGGTAGGGCTGGGAAGCCGATCGGCGCCAAAGGCTTCGGCCGTACCCAAACAATTCCGATTCGGAGTACCGTGGTTCATCGAGCGGAAACGTGTGCTGC
CTGCGATGCTGCCCTTCCCTTGGATGCTCGATTTATAGCTCGAATCGGCTATGTGACCATCGATCTGATAAGGGGTGGCCCGGATCAGCCTGGATTGAGG
ATGGAGGGAACCAAGCACCTTTTTGGGGAAATTGCCTGTAGATGCGGCCATATTTCATGTACCGGTCCCGGCACAGGGGATCGACTTGAGATTGCAGGAC
GCAAAACCGCAACCAGTTTGAGTGAATGGCGTATTGTCGGCCCCATGTTGGCAGCTTTCATTGTTGCGCTCGCAAAACGGATGCGGTTGTCTCGCTCCAA
GGTCCAGGAGTTTTTAATCGATTGGTTCGGCCTGGAGTTGAGTGTCGGGACCATCGACAATTGCATACGTGAAGTCGGGCTGGCTGCCAACCCTGTTCAG
GATGAACTGATTGCTGAACTTCGTGCTTCTGCTCTGGCTCATGTAGACGAAACGCCTTGGAAGCAAAAAGGTCAAGCTTTGTGGCTTTGGGTTTTCGCGA
CGGCCAATACAGTCTTGTTTTGTGTGGGACCTCGGACACGGCAAATGGTCATGGATATCCTGACCGAGGAATTCGCGGGCTGGCTGATGAGCGACGGACT
CATGAACTACCGGACTTTTTCAAAGCGGCTGCGTTGCTGGGCACATCTGATCCGGAAGGCCAAAGGGTTGTCGGTAAGCCTGGATAAAGAGACCAAGCGA
TTCGGCTCCAGGGTATTGGAAGTCCTGGAATACATGGTCGAAGAGGTCAAAGACGGATCGGATCCGCCTGCTGCCGCGTCGATTTTGGAAGATTTCCAGG
GATACTGCAAAATGTACAGAGATTATCCCCCGTCCGAAAAGGTTCGCAGCCTTGCCGTTGAGCTTCTCAATGACTGGGATGTCATCTGGCTACCCTTGAG
ATCTCCGGGGTTGCCACTGACAAACAATGATGCTGAACGTGCGCTGCGCCACTGGGTCATCGCGCGTTTGATCAGCCATGGAACTCGTACGGCAGAAGGG
AGCCAAGTATTGGGTGTGCTGGCTAGCGTCATCGAAACTTGCCGATTGCGGAACGTGTCCCCCTGGGACTATCTTGCTAAGGTAATTGCTGAACGGAGAA
ATGGAAACCCCGTACCACCGTTACCGGCTCCAGTAGCCGCTTGATATCGGGGTCTCTGAACGGTTAC

Protein section

ORF number : 1

ORF 1

Length		Begin	End	Strand	Fusion ORF
1599 bp	532 aa	346	1944	+	No

ORF function : Transposase
Chemistry : DDE

ORF sequence :

MKLSDHDLLQIDEEYLSSLSSAELLAVSRKMLEDLKESRERLNRTPDNSSQPPSSRPAYLGIPVEEDEAHLDEDEDDASPDKKKGADDEGGDDSPGPSGN
STPPAPGKGKPDSSSEKPKGRAGKPIGAKGFGRTQTIPIRSTVVHRAETCAACDAALPLDARFIARIGYVTIDLIRGGPDQPGLRMEGTKHLFGEIACRC
GHISCTGPGTGDRLEIAGRKTATSLSEWRIVGPMLAAFIVALAKRMRLSRSKVQEFLIDWFGLELSVGTIDNCIREVGLAANPVQDELIAELRASALAHV
DETPWKQKGQALWLWVFATANTVLFCVGPRTRQMVMDILTEEFAGWLMSDGLMNYRTFSKRLRCWAHLIRKAKGLSVSLDKETKRFGSRVLEVLEYMVEE
VKDGSDPPAAASILEDFQGYCKMYRDYPPSEKVRSLAVELLNDWDVIWLPLRSPGLPLTNNDAERALRHWVIARLISHGTRTAEGSQVLGVLASVIETCR
LRNVSPWDYLAKVIAERRNGNPVPPLPAPVAA

Blast result :

Comments

ISMasp5 is 95% aa similar to ISMasp4.

References

1] Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Goodwin,L.A., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N. and Richardson,P. (2006) Direct Submission GenBank.

2] ISfinder annotation (2008).