ISMasp6
- Family IS5
- Group IS427
Isoform Synonym(s)
| Accession number | Transposition | Origin | Host |
|---|---|---|---|
| NC_008576 | ND | Magnetococcus sp. | Magnetococcus sp. MC-1 |
DNA section
IS Length : 834 bp
Ends
IR Length : 19
IRL : GATACTGTCGTCACAAGATAAGCTGATGTAGAATAGGAGTCAAAGCAAGG
IRR : GATACTGTCGTCACAAGATTGCGCCCCAGATGGCAATACACCTGATTTGT
Insertion site
| Left flank | Direct repeat | Right flank | DR Length |
|---|---|---|---|
| TGGAGGATCT | TA | AGGAATCTCG | 2 |
DNA sequence
GATACTGTCGTCACAAGATAAGCTGATGTAGAATAGGAGTCAAAGCAAGGAGGCTTTGACATGACTGCATCTTACCGTAGGCACGATATTTCAGACCAAC
TATGGTCACGCCTTGAGCCCCATTTGCCTGGAAGGGCTGGAAGCTGGGGAGGTGTAGCCAAGGACAACCGCCTTTTTATCAATGCGGTGTTTTGGATCTT
GCGTACAGGTGCGCCATGGCGCGATCTTCCACCAGATTATGGCGATTGGAACAACACTCAACGCCGTTTCTGCCGGTGGCGCGACAAAGGGATCTGGGAG
AAGCTGCTTGACCAGATGATTGAAGACCCTGATTACGAGTGGTTGATGATCGATGCATCTCACTGCAAGGTGCATCCGCATGCAGCTGGGGCCAAAGGCG
GCAATCAAGAGATGAGTCGCACAAAAGGGGGCTCAACAGTAAGATACATTTGGCCGTGGATGCGCATGGTATGCCGGTCAGAGCGATTATCACGAAGGGT
ACCCGAGCTGATTGCTCACAAGCTCAAGCCTTGATATCGGGTTTTGAAGCAGAGTGCTTGCTGGCTGACAGGGGTTATGACAGCGACGCTATAGTTGAGC
AAGCTTGCGAACAGGGAATGAAGCCAGTGATCCCGCCGCGCAAAAACAGGAAAACTCCACGAGTGTATGATGTGGATCTCTATAAAATCCGTCACTTGGT
AGAGAATGCATTTTTGCATTTGAAAAGATGGCGAGGCATCGCCACCAGATACGCCAAGAACGTAGATTCATTCCTGGCGGCGGTACAAATCAGGTGTATT
GCCATCTGGGGCGCAATCTTGTGACGACAGTATC
TATGGTCACGCCTTGAGCCCCATTTGCCTGGAAGGGCTGGAAGCTGGGGAGGTGTAGCCAAGGACAACCGCCTTTTTATCAATGCGGTGTTTTGGATCTT
GCGTACAGGTGCGCCATGGCGCGATCTTCCACCAGATTATGGCGATTGGAACAACACTCAACGCCGTTTCTGCCGGTGGCGCGACAAAGGGATCTGGGAG
AAGCTGCTTGACCAGATGATTGAAGACCCTGATTACGAGTGGTTGATGATCGATGCATCTCACTGCAAGGTGCATCCGCATGCAGCTGGGGCCAAAGGCG
GCAATCAAGAGATGAGTCGCACAAAAGGGGGCTCAACAGTAAGATACATTTGGCCGTGGATGCGCATGGTATGCCGGTCAGAGCGATTATCACGAAGGGT
ACCCGAGCTGATTGCTCACAAGCTCAAGCCTTGATATCGGGTTTTGAAGCAGAGTGCTTGCTGGCTGACAGGGGTTATGACAGCGACGCTATAGTTGAGC
AAGCTTGCGAACAGGGAATGAAGCCAGTGATCCCGCCGCGCAAAAACAGGAAAACTCCACGAGTGTATGATGTGGATCTCTATAAAATCCGTCACTTGGT
AGAGAATGCATTTTTGCATTTGAAAAGATGGCGAGGCATCGCCACCAGATACGCCAAGAACGTAGATTCATTCCTGGCGGCGGTACAAATCAGGTGTATT
GCCATCTGGGGCGCAATCTTGTGACGACAGTATC
Recoding section
- Recoding by frameshift
- Frame
- Type
- Experimentally demonstrated
Stimulators :
- Shine-Dalgarno sequence :
- Secondary structure :
Recoding motif :
Protein section
ORF number : 3
ORF 1
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 474 bp | 157 aa | 61 | 534 | + | No |
Description : First part of the transposase
ORF sequence :
MTASYRRHDISDQLWSRLEPHLPGRAGSWGGVAKDNRLFINAVFWILRTGAPWRDLPPDYGDWNNTQRRFCRWRDKGIWEKLLDQMIEDPDYEWLMIDAS
HCKVHPHAAGAKGGNQEMSRTKGGSTVRYIWPWMRMVCRSERLSRRVPELIAHKLKP
HCKVHPHAAGAKGGNQEMSRTKGGSTVRYIWPWMRMVCRSERLSRRVPELIAHKLKP
Blast result :ORF 2
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 492 bp | 163 aa | 333 | 824 | + | No |
Description : Second part of the transposase
ORF sequence :
LRVVDDRCISLQGASACSWGQRRQSRDESHKRGLNSKIHLAVDAHGMPVRAIITKGTRADCSQAQALISGFEAECLLADRGYDSDAIVEQACEQGMKPVI
PPRKNRKTPRVYDVDLYKIRHLVENAFLHLKRWRGIATRYAKNVDSFLAAVQIRCIAIWGAIL
PPRKNRKTPRVYDVDLYKIRHLVENAFLHLKRWRGIATRYAKNVDSFLAAVQIRCIAIWGAIL
Blast result :ORF 3
| Length | Begin | End | Strand | Fusion ORF | |
|---|---|---|---|---|---|
| 764 bp | 254 aa | 61 | 824 | + | Yes |
Chemistry : DDE
ORF sequence :
MTASYRRHDISDQLWSRLEPHLPGRAGSWGGVAKDNRLFINAVFWILRTGAPWRDLPPDYGDWNNTQRRFCRWRDKGIWEKLLDQMIEDPDYEWLMIDAS
HCKVHPHAAGAKGGNQEMSRTKRGLNSKIHLAVDAHGMPVRAIITKGTRADCSQAQALISGFEAECLLADRGYDSDAIVEQACEQGMKPVIPPRKNRKTP
RVYDVDLYKIRHLVENAFLHLKRWRGIATRYAKNVDSFLAAVQIRCIAIWGAIL
HCKVHPHAAGAKGGNQEMSRTKRGLNSKIHLAVDAHGMPVRAIITKGTRADCSQAQALISGFEAECLLADRGYDSDAIVEQACEQGMKPVIPPRKNRKTP
RVYDVDLYKIRHLVENAFLHLKRWRGIATRYAKNVDSFLAAVQIRCIAIWGAIL
Blast result :
Comments
ISMasp6 is 59% aa similar to ISCc1. The third ORF is a putative ORFAB transposase reconstructed in silico by possible -1 frameshift.
ISMasp6 is located inside ISMasp5.
ISMasp6 is located inside ISMasp5.
References
1]Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S., Kiss,H., Goodwin,L.A., Brettin,T., Bruce,D., Han,C., Tapia,R., Gilna,P., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Mikhailova,N. and Richardson,P. (2006) Direct Submission GenBank.